minus-DHMEQ-Dehydroxymethylepoxyquinomicin-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemE(-)-DHMEQCat. No.: HY-14645CAS No.: 287194-40-5Synonyms: Dehydroxymethylepoxyquinomicin分式: CHNO分量: 261.23作靶点: NF-B作通路: NF-B储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 32 mg/mL (122.50 m

2、M)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.8280 mL 19.1402 mL 38.2804 mL5 mM 0.7656 mL 3.8280 mL 7.6561 mL10 mM 0.3828 mL 1.9140 mL 3.8280 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 (-)-DHMEQ is prepared in DMSO and then diluted with saline

3、2.BIOLOGICAL ACTIVITY物活性 ()-DHMEQ种有效的 NF-B inhibitor。IC50 & Target RelA RelB1/2 Master of Small Molecules 您边的抑制剂师www.MedChemE体外研究 ()-DHMEQ binds to cysteine 38 of RelA and cysteine 144 of RelB. ()-DHMEQ at concentrations greaterthan 25M inhibits the LT-induced nuclear translocation of FLAG-RelB WT,

4、whereas the inhibitory effect of()-DHMEQ on the nuclear translocation of FLAG-RelB (C144S) became weaker at the same concentrations1.PROTOCOLCell Assay 1 A549 cells transfectants stably expressing RelB WT and RelB (C144S) are preincubated with variousconcentrations of (-)-DHMEQ (1, 5, 10, 25, and 50

5、 M) for 1h and then incubated with or without LT (25ng/mL) for 2 h in the presence or absence of (-)-DHMEQ.Cytoplasmic and nuclear extracts are analyzed byWestern blotting 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Theranostics. 2019

6、Jan 1;9(2):436-448. Sens Actuators B Chem. 2018 Nov 20;274:481-490. Anal Chem. 2017 Jul 18;89(14):7316-7323. Anal Chim Acta. 2019 Feb 7;1048:168-177. Mol Cancer Ther. 2018 Jun;17(6):1303-1314.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Quach HT, et al. Eudesmane-Type Sesqui

7、terpene Lactones Inhibit Nuclear Translocation of the Nuclear Factor B Subunit RelB inResponse to a Lymphotoxin Stimulation. Biol Pharm Bull. 2017;40(10):1669-1677.2. Kang J, et al. Exposure to a combination of formaldehyde and DINP aggravated asthma-like pathology through oxidative stress and NF-B activation. Toxicology. 2018 May 14;404-405:49-58.McePdfHeightCaution: Product has not been fully validated for medical applications.For researc