各种激发光和吸收光波长

1、CONFOCAL去做多重染色时是现在发现一个更全的有关激发光和发射光的波长图谱。这对 必不可少的参考资料。全部文件Excitatio n and Emissi on Wavele ngths of Fluorop hores Tavi's "FluoroTable": Com mon fluoro phoresEmissi on Other info(from )1,5 IAEDANS3364901,8-ANS3724804-Methylumbellifero ne3855025-carboxy-2,7-dichlorofluoresce in5045295-Car

2、boxyfluorescei n (5-FAM)4925185-Carbox ynap thofluoresce in (pH 10)512/598563/6685-Carboxytetramethylrhodam ine542568(5-TAMRA)5-FAM (5-Carboxyfluorescei n)4925185-HAT (Hydroxy Tryp tami ne)370-415520-5405-Hydroxy Tryp tami ne (HAT)370-415520-5405-ROX (carboxy-X-rhodami ne)5786045675915-TAMRA548552(5

3、-Carboxytetramethylrhodam ine)5425686-Carboxyrhodam ine 6G5185436-CR 6G518543Fluoro phoreAbso rp ti onRatio Dye, pH6-JOE5205487-Ami no-4-methylcouman n3514307-Ami noact in omycin D (7-AAD)5466477-Hydroxy-4-methylcoumari n360449, 4559-Ami no-6-chloro-2-methoxyacridi ne412,430471,474ABQ344445Acid Fuch

4、s in540630ACMA412,430471,474(9-Ami no-6-chloro-2-methoxyacridi ne)Acridi ne Ora nge + DNA502526Acridi ne Ora nge + RNA460650Acridi ne Ora nge, both DNA & RNA440-480520-650Acrid ine Red455-600560-680Acridi ne Y ellow470550Acriflav in436520Acriflavi n Feulgen SITSA355-425460Aequori n (P hot op rot

5、ei n)466Phot opr ote inAFPsAutoFluoresce ntProtein(Quantum Biotechnologies)see sgGFP,sgBF PAlexa Fluor 350346442342441Alexa Fluor 430431540Alexa Fluor 488495, 492519, 520Alexa Fluor 532531, 532553, 554Alexa Fluor 546556, 557572, 573Alexa Fluor 568577,578603Alexa Fluor 594590, 594617, 618Alexa Fluor

6、633632650Alexa Fluor 647647666Alexa Fluor 660668698Alexa Fluor 680679702Alizari n Comp lex on530-560,580,580624-645Alizari n Red530-560580Allo phycocya nin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Ami no methylcoumari n)345425347444AMCA-X353442Ami no acti no myci n D555655Amino coumari n346442350

7、445Ami no methylcoumarin (AMCA)345425347444Ani lin Blue600An throcyl stearate360-381446APC (Allo phycocya nin)630, 645655, 660AP C-Cy7625-650755AP TRA-BTC = Ratio Dye, Zn2+466/380520/530 Ratio Dye, Zn2+APTS424505Astrazon Brillia nt Red 4G500585Astraz on Orange R470540Astrazon Red 6B520595Astrazon Y

8、ellow 7 GLL450480Atabri ne436490ATTO-TAG? CBQCA465560ATTO-TAG? FQ486591Auramine460550Aurop hos phine G450580Aurop hos phine450-490515BAO 9(Bisam inophenyl oxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberi ne Sulp hate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y 66H

9、)381,382, 445,blue shifted GFPBlue Fluoresce nt Prote in383447, 448(Y 66H)BlueFluoresce ntProteinBFP/GF P FRETBima ne398490Bisbe nzamide360461Bisbe nzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+Blancophor FFG390470Bla ncop hor SV370435BOBO?-1462481BOBO?-3570602Bodi py

10、492/515490515Bod ipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodi py 542/563543563Bodipy 558/568558569Bodi py 564/570564570Bodipy 576/589579590Bodi py 581/591584592Bodi py 630/650-X625642Bodi py 650/665-X647665Bodi py 665/676605676Bodi py Fl504,505511, 513Bodi py FL

11、ATP505514Bod ipy Fl-Ceramide505511Bodi py R6G SE528547Bodi py TMR542574Bod ipy TMR-X conjugate544573Bodi py TMR-X, SE544570Bodi py TR589617Bodi py TR ATP591620Bodi py TR-X SE588616BO-PRO?-1462481BO-P RO?-3544570Brillia nt Sul phoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529 Ratio Dye Ca2+BTC-5N

12、- atio Dye, Zn2+459/417517/532 Ratio Dye, Zn2+Calce in494517Calcei n Blue373440Calcium Crims on588, 589611,615Calcium Green531Calcium Gree n-1 Ca2+ DyeCalcium Gree n-2 Ca2+Calcium Gree n-5N Ca2+Calcium Gree n-C18 Ca2+Calcium OrangeCalcofluor WhiteCarboxy-X-rhodami ne (5-ROX)Cascade BlueCascade Y ell

13、owCatecholam ineCCF2 (Gen eBlazer)CFDACFP - Cyan Fluoresce nt P rotein506531506/503536506532509530549575576385,395, 437,405440, 445576601377420398423399399550400552410470494520430,433, 474,501, 506Ca2+ DyeCa2+Ca2+Ca2+Cyan Fluoresce nt436, (453)475,Protein476,(501)CFP/YFP FRETChloro phyll480650Chromo

14、myc in A436-460470Chromomyc in A445575CL-NERF (Ratio Dye, pH)504/514540Ratio Dye, pHCMFDA494520Coele nteraz ineCa2+Dye, (429)465Ca2+Dye,biolu min esce neebiolu min esce nee,n ative moleculeCoele nteraz ine cp (Ca2+ Dye,)(430)442Ca2+Dye,biolu min esce neeCoele nteraz ine f(437)473Ca2+Dye,biolu min es

15、ce neeCoele nteraz ine fcp452Ca2+Dye,biolu min esce neeCoele nteraz ine h(437)464Ca2+Dye,biolu min esce neeCoele nteraz ine hep(433)444Ca2+Dye,biolu min esce neeCoele nteraz ine ip441Ca2+Dye,biolu min esce neeCoele nteraz ine n(431)467Ca2+Dye,biolu min esce neeCoele nteraz ine O460575Coumari n P hal

16、loidin387470C-p hycocya nineCPM Methylcoumar in384469CTC400-450602CTC Formaza nCy2489506Cy3.1 8554568Cy3.5581598Cy3514566552570554Cy5.1 8649666Cy5.5675695Cy5649666670Cy7710, 743767Cyan GFP433 (453)475(50,cyclic AMP Fluorose nsor (FiCRhR)5001 JU517CyQua nt Cell P roliferati on Assay480520,805Methylco

17、umari nCellProliferatio nAssayDabcyl453Dan syl340578Dan syl Ami ne337517Dan syl Cadaveri ne335518Dan syl Chloride372518Dan syl DHPE336517Dan syl fluoride356nonDAPI359461Dapo xyl403580Dapo xyl 2374574Dapo xyl 3373574DCFDA504529DCFH(Dichlorodihydrofluoresce in505535Diacetate)DDAO463607DHR (Dihydorhoda

18、mi ne 123)505534Di-4-ANE PPS496705Di-8-ANE PPS (non-ratio)488605498713DiA (4-Di-16-AS P)456591Dichlorodihydrofluoresce inDiacetate 505535(DCFH)DiD - Lipop hilic Tracer644665DiD (DilC18 (5)644665DIDS341415Dihydorhodami ne 123 (DHR)505535DiI (DiIC18 (3)549, 551565Din itr ophenol349DiO (DiOC18(3)484, 4

19、87501, 502DiR748780DiR (DiIC18 7)750779DM-NERF (high pH)497/510540DNP349Dopamine340490-520DsRed558583DTAF494520DY-630-NHS621660DY-635-NHS634664Lipop hilic TracerLipop hilic TracerRatio Dye, pHproteinHemicya nelabelprotei ns and DNAHemicya neRedfluoresce ntforlabelforEBF P383447ECF P436474EGF P488, 4

20、98507, 516ELF 97345530Eosin524545protei ns and DNAEnhancedFluoresce ntProteinEnhancedFluoresce ntProteinEnhancedFluoresce ntP roteinBlueCya nGreenErythrosi n529, 532554,555Erythrosi n ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617Euchrys in430540EukoLightEuropium (III)

21、 chlorideEYFP513, 520527,532EnhancedY ellowFluoresce ntP roteinFast Blue360440FDA494520Feulge n (P ararosa nili ne)570625FIF (Formaldehyd In duced Fluoresce nee)405433FITC490, 494520, 525FITC An tibody493517Flazo Orange375-530612Fluo-3480?506, 520, 527506Fluo-4494516Fluoresce in (FITC)490, 494520, 5

22、25Fluoresce in Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidi ne)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red?(high pH)572657Fura Red?Fluo-3Fura-2, high calcium335505Excitatio nratiodyeFura-2, low calcium363512Fura-2/BCECFGe nacryl Brillia nt Red B520590Ge

23、nacryl Brillia nt Y ellow 10GF430485Gen acryl Pink 3G470583Gen acryl Y ellow 5GF430475Gen eBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGF P)498516GFP wild type,non-UVexcitati on475509(wtGF P)GFP wild type, UV excitation (wtGF P)395509GFP uv385508Gloxalic Acid405460Granu lar Blue355425Haemat oporp hyri n530-560580Hoechst 33258345487Hoechst 33342347Excitatio nratiodyeFilter sets in stalled on the Axiovert 200M:1)Brightfie