生物反应器Newtechnologiesincellculture实用教案

1、Content Introduction Comparison Sensor technologies第1页/共34页第一页，共33页。Introduction - bioreactors第2页/共34页第二页，共33页。Introduction - bioreactors Capable of being operated aseptically for a number of days, weeks and even months Capable of generating repeatable process conditions for a number of days, weeks

2、and even months Adequate aeration and agitation should be provided to meet the metabolic requirements of the micro-organism or cell pH, Oxygen, Temperature and other control should be provided Sampling facilities should be provided Should be suitable for a range of processes Should be constructed to

3、 ensure smooth internal surfaces (cleanability)第3页/共34页第三页，共33页。-Flask第4页/共34页第四页，共33页。 Test Tubes, Shake flasks. Increased aeration. Reduced evaporative loss. Solid support , Re-useable / Autoclavable. May be used with the SensorDish Reader.-Flasks are Shake Flasks第5页/共34页第五页，共33页。 -Flask Configura

4、tionsFrom 100ul to 4.0ml第6页/共34页第六页，共33页。SensorDish Reader , SDR第7页/共34页第七页，共33页。SensorDish Reader , SDR1.Non-invasive, online.2.pH or Dissolved Oxygen.3.No Calibration Required.6-well24-well第8页/共34页第八页，共33页。ExpandableAdditional SDRs link into the Basic Set.Up to 10 SDRs on one Laptop.No additional

5、software required for expansion.第9页/共34页第九页，共33页。SDRs Integrate with -Flasks-Flasks Integrate with the SensorDishReader1.Eliminate Well-plate inconsistency.2.Reduce and equalize evaporative losses across well-plates.3.Obtain online pH or dissolved oxygen data for all wells.第10页/共34页第十页，共33页。DO profi

6、les (all 24 for each well-plate)Polystyreneu-Flask第11页/共34页第十一页，共33页。SensorFlaskReader , SFR第12页/共34页第十二页，共33页。The SensorFlask Reader, SFR Shaker-mounting blue-tooth sensor platform. Shake Flasks with DO and pH sensors.(also single DO or pH if desired) Multiple volumes (125/250/500/1000 ml). Up to 9

7、 flasks on the platform at one time.第13页/共34页第十三页，共33页。Microbial and Mammalian Applications Microbial Applications:Monitor pH and DO levels. Ensure aerobic growth.Follow carbon source utilization and exhaustion.Adjust orbiter speeds to maintain DO levels for better scalability. Mammalian Application

8、s: Accurately monitor pH /pCO2 levels. Adjust bicarbonate levels more accurately.Monitor DO levels, adjust orbiter speeds for better scalability.第14页/共34页第十四页，共33页。Software第15页/共34页第十五页，共33页。Individual Flask Calibration第16页/共34页第十六页，共33页。Complete Hardware Hardware Includes:Platform.Batteries.Bluetoo

9、th adapter.Software.Clamps. Bluetooth range of 15ft. Battery life 3 months on a single charge. (platform can operate on single battery)第17页/共34页第十七页，共33页。The RAMBIO1000 (Resonant Acoustic Mixing)第18页/共34页第十八页，共33页。Resonant Acoustic Mixing All energy goes into mixing the contents of the flask 6 fold

10、better OTRs than orbital shakers due to increased headspace exchange Up to 50% fill volume in standard flasks第19页/共34页第十九页，共33页。The Applikon RAMAccessories: Clamps and Oxy-pump Stoppers250ml1000ml第20页/共34页第二十页，共33页。Single use applications Small scale options, more R&DAppliFlex CellReady第21页/共34页

11、第二十一页，共33页。Sensor technologies Traditional sensor pH and DO Sterilized via autoclave第22页/共34页第二十二页，共33页。Sensor technologies - Fluorescent Fluorescent light is measure for pH and DO第23页/共34页第二十三页，共33页。Fluorescence pro/con Pre-sterilized Pre-calibrated (pH) Single use + reusable (DO) Measurement throu

12、gh wall of vessel pH range limited Not backwards compatible (controller) Can be influenced by medium (pH) Additional costPROCON第24页/共34页第二十四页，共33页。New technologies AppliFlex reusableAppliFlex single sensorsuse sensor第25页/共34页第二十五页，共33页。New technologies Reusable standard Single sensors use Sensor optionalEz-Control第26页/共34页第二十六页，共33页。Small scale bioreactors第27页/共34页第二十七页，共33页。Small scale bioreactor system28第28页/共34页第二十八页，共33页。Small scale bioreactor Communication via web browser Software to do calibration/setpoints/ control loops on mult