BALB_c小鼠肾缺血再灌注损伤模型的建立与评价_胡红林

1、中国组织工程研究与临床康复 第15卷 第5期 20110129出版Journal of Clinical Rehabilitative Tissue Engineering Research January 29, 2011 Vol.15, No.5BALB/c小鼠肾缺血再灌注损伤模型的建立与评价胡红林1，王共先2，邹 丛3，习小庆1，史子敏1Establishment and evaluation of renal ischemia/reperfusion injury models in BALB/c miceHu Hong-lin1, Wang Gong-xian2, Zou Cong3

2、, Xi Xiao-qing1, Shi Zi-min1AbstractDepartment of Urinary Surgery, Second AffiliatedHospital of Nanchang University, Nanchang 330006, Jiangxi Province, China; 2Department ofUrinary Surgery, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China; 3Department of End

3、ocrinology, Fourth AffiliatedHospital of Nanchang University, Nanchang 330003, Jiangxi Province, ChinaHu Hong-lin, Doctor, Attending physician, Department of Urinary Surgery, Second AffiliatedHospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaCorrespondence to: Hu Hong-lin, Doc

4、tor, Attending physician, Department of Urinary Surgery, Second AffiliatedHospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaReceived: 2010-07-12 Accepted: 2010-09-12BACKGROUND: For studies of some drugs, mouse serves as an ideal animal for model establishment. However, the fai

5、lure rate is high due to poor toleration, small size of the kidney and renal pedicle.OBJECTIVE: To construct the model of renal ischemia/reperfusion injury (IRI) in BALB/c mice and evaluate influences of different ischemic time on IRI.METHODS: Renal arteries of mice were bilaterally clamped with mic

6、ro-artery clamps to establish model of renal IRI in maleBALB/c mice. According to different ischemic time, the mice were divided into four groups: 0 (control), 30, 35, and 45 minutes. The animals were sacrificed at 24 hours post-operatively. Renal function and pathology of the kidneys were examined.

7、 The situation of illness and survival in 45-minute group were observed.RESULTS AND CONCLUSION: The successful rate of model was 95.9%. Levels of serum creatinine and blood urea nitrogen as well as histological scores were remarkably increased in 30-, 35-, and 45-minute groups compared with control

8、group 24 hours after operation (P < 0.05). However, the survival rate was significantly lower in 45-minute group (P < 0.05). Results show that stable model of renal IRI can be gained in BALB/c mice by applying micro-artery clamp to incarcerate bilateral renal arteries. The optimal renal ischem

9、ia time is 35-45 minutes in male mice.摘要背景：对于一些药物研究，小鼠是理想的造模工具，但由于小鼠耐受性相对较差，肾脏及肾蒂小且难于寻找，容易增加实验误差，导致造模失败。目的：探讨BALB/c小鼠肾缺血再灌注损伤模型的建立方法，评价肾脏缺血时间对肾缺血再灌注损伤的影响。方法：采用微型动脉夹夹闭小鼠双侧肾蒂的方法建立雄性BALB/c小鼠肾缺血再灌注损伤模型，根据肾缺血时间不同分为 0 min组(对照组)、30 min组、35 min组、45 min组，肾再灌注后24 h观察肾功能和肾脏病理组织学的变化，比较不同的肾脏缺血时间对上述指标的影响；观察45 min

10、组小鼠肾缺血再灌注损伤后的生存率。结果与结论：模型成功率95.9%，与对照组相比，肾缺血30 min组、35 min组和45 min组再灌注后24 h血清肌酐、尿素氮和肾脏病理组织学评分均升高，肾缺血45 min组生存率明显下降，差异均有显著性意义(P < 0.05)。结果提示，应用微型动脉夹夹闭小鼠双侧肾蒂的方法可制备稳定肾缺血再灌注损伤模型，雄性小鼠肾缺血3545 min是造模较为理想的肾缺血时间，所得模型效果满意。0 引言 肾缺血再灌注损伤(renal ischemia- reperfusion injury，IRI)是缺血性急性肾功能衰竭的主要发病机制，具有较高的发病率和死亡率1-2，伴随着一系列连贯的细胞事件发生，包括活性氧(ROS)释放、凋亡、坏死、炎症细胞的浸润和活性介质的释放，从而导致组织损伤。动物实验是开展肾IRI研究的常用方法，合格的动物模型建立是实验研究成功的基础和关键。作者应用微型动脉夹夹闭双侧肾蒂方法制备BALB/c小鼠IRI模型，取得较为满意的效果，并观察了不同的肾脏缺血时间对肾IRI的影响。31 材料和方法设计：单一样本观察。时间及地点：实验