微生物实验7 病毒形态学和培养及血清学

VirusDemonstrationFungi:morphology,cultureLaboratorydiagnosisofviraldiseasesbymorphological

methodMorphology（electronmicroscope）Adenovirus,HerpesvirusCPE（cytologicexamination）uninfectedcells,infectedcellsInclusionbodiesofRabiesvirusandMeaslesvirusOperationHemagglutinationassayHemagglutinationinhibitiontestVideo:Animalexperiment22023/3/28InclusionbodiesInclusionbodiesarestainablestructureswhichareeitherhistologicchangesinthecellscausedbyviralcomponentsorvirus-inducedchangesincellstructures.Theymayappearwithinthenucleusorcytoplasm,andtheycanfacilitatelaboratorydiagnosis32023/3/28InclusionbodiesofRabiesvirus（Negribody）Negribodyinaneuron

42023/3/28InclusionbodiesofRabiesvirus（Negribody）52023/3/28InclusionbodiesofMeaslesvirus62023/3/28CPECelldeathCellrounding,degeneration,aggregation,lossofattachmentstotheculturedishCharcteristichistologicchangesInclusionbodies,marginationofchromatinSyncytiaCellsurfacechanges72023/3/28UninfectedVerocellsCPEofHSVinfectionHSV-1-infectedVerocells82023/3/28HARBChemagglutinationVIRUSRBCInfluenzavirusMumpsvirusParainfluenzavirusNewcastlediseasevirusAdenovirusEnterovirusMeaslevirusReovirusHemagglutinationassay92023/3/28102023/3/28112023/3/28Hemagglutinationinhibitiontest(HITest)procedureforthemeasurementofserumantibodiesdirectedagainstahemagglutinatingvirus;thehighestdilutionofserumthatcompletelyinhibitshemagglutinationbyastandardizedviralpreparationisreportedasthehemagglutinationtiter.Detectionofantibodyorserotypingofvirus122023/3/28No.12345678910Saline（ml）Virus（ml）0.2+0.20.2+0.20.2+0.20.2+0.20.2+0.20.2+0.20.2+0.20.2+0.20.2+0.20.2dilution1∶101∶201∶401∶801∶1601∶3201∶6401∶12801∶2560controlsaline（ml）Each0.2ml0.5%RBCsuspension（ml）Each0.4mlProcedureofhemagglutinationassayLabelthewellsfromNo.1to10.Add0.2mlofsalinetoeachwells.Dilutethevirusaccordingthetablebelow.Add0.2mlofsalinetoeachwellsfromNo.10toNo.1.Add0.4mof0.5%RBCsuspensiontoeachwellsfromNo.10toNo.1.Shaketomix

andincubateatRTfor45min.0.2discardDeterminationofHAUtiter132023/3/284Hemagglutinatingunit(HAU)1HAU=thehighestdilutionof“++”1HAU=1:80→4HAU=1:20142023/3/28NO.12345678control9control10controlsaline（ml）serum（ml）0.2+0.20.2+0.20.2+0.20.2+0.20.2+0.20.2+0.20.2+0.20.2+0.20.20.4Dilutionofserum1∶101∶201∶401∶801∶1601∶3201∶6404HAUvirus（ml）Each0.2ml0.20.5%RBCsuspension（ml）Each0.4ml0.4LabelthewellsfromNo.1to10.Add0.2mlofsalinetoeachwellsfromNo.1toNo.9,add0.4mlofsalinetoNo.10.Dilutetheserum(antibody)accordingthetablebelowfromNo.1toNo.7,addtheserumtoNo8wellasthecontrol.Add0.2mlof4HAUvirustoeachwellsfromNo.7toNo.1,addthevirustoNo9wellasthecontrol.Shaketomix,thenAdd0.4mof0.