Bromobimane-Monobromobimane-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEBromobimaneCat. No.: HY-100041CAS No.: 71418-44-5Synonyms: Monobromobimane分式: CHBrNO分量: 271.11作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 86.6 mg/mL (319.43 mM)* m

2、eans soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 3.6885 mL 18.4427 mL 36.8854 mL5 mM 0.7377 mL 3.6885 mL 7.3771 mL10 mM 0.3689 mL 1.8443 mL 3.6885 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作

3、液，建议您现现配，当天使；澄清的储备液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.08 mg/mL (7.67 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.08 mg/mL (7.67 mM); Clear solution1/2 Master of Small Molecules 您边的抑制剂师ww

4、w.MedChemEBIOLOGICAL ACTIVITY物活性 Bromobimane本上 荧光物质，当与硫醇反应时，成荧光产物。体外研究 Bromobimanes in solution (aqueous or organic solvents of medium polarity) react with small thiols e.g., thetripeptide thiol glutathione (GSH), and with reactive protein thiol groups (e.g., hemoglobin). The reactions ofbromobimane

5、s with thiols are second order and dependent on pH, the active nucleophile being the thiolateanion. The reaction of bromobimane with a thiolate converts the nonfluorescent agent into water-solublefluorescent products. The neutral agents mBBr and bBBr are moderately soluble in mediumpolarity organics

6、olvents (acetonitrile, dichloromethane), and slightly soluble in water. The quaternary salt, qBBr, and theanionic bromobimane, SBBr, are soluble in water, but less soluble in organic solvents. Bromobimanes areyellow 1. The highly selective, rapid reactivity of bromobimanes toward thiols, the stabili

7、ty and fluorescenceyield of the thiol derivatives, the ease of separation of the derivatives by reversed-phase HPLC, and theavailability of both cell-penetrating and nonpenetrating forms, make the use bromobimanes an extremelypowerful approach to the analysis of low molecular weight biothiols 2.PROT

8、OCOLCell Assay 2 The stock mBBr solution is prepared by weight at 100-180 mM in acetonitrile. The stock solution of SBBr isprepared by weight at 50 mM in dimethyl sulfoxide (DMSO) or at 2 mM in the 20 mM Tris-methane sulfonate.The thiol is added to a final concentration of 1 mM to a solution of 2 mM

9、 mBBr or SBBr, 20 mM Tris-methanesulfonate, pH 8.0. The mixture is incubated in the dark for 15 min at room temperature and thenmethanesulfonic acid is added to 25 mM from a 5 M stock solution 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Kosower EM, et al. Bromobimane probes for thiols. Methods Enzymol. 1995;251:133-48.2. Newton GL, et al. Determination of biothiols by bromobimane labeling and high-performance liquid chromatography. Methods Enzymol.1995;251:148-66.McePdfHeightCaution: Product has not been fully validat