“标本配穴”电针预处理对心肌缺血再灌注模型大鼠心肌细胞骨架蛋白的影响

“标本配穴”电针预处理对心肌缺血再灌注模型大鼠心肌细胞骨架蛋白的影响“标本配穴”电针预处理对心肌缺血再灌注模型大鼠心肌细胞骨架蛋白的影响”

摘要：心肌缺血再灌注损伤是一种常见且严重的心肌病理生理过程，而电针预处理作为一种先进的治疗方法在心血管领域具有广泛的应用。本研究旨在探究“标本配穴”电针预处理对心肌缺血再灌注模型大鼠心肌细胞骨架蛋白的影响。

方法：将40只雄性SD大鼠随机分为对照组、心肌缺血再灌注组和电针预处理组，每组10只。对照组大鼠不进行任何操作，心肌缺血再灌注组和电针预处理组大鼠采用左前降支结扎法制备心肌缺血再灌注模型。电针预处理组在心肌缺血再灌注手术前进行电针预处理，使用频率为10Hz，电流幅度为2mA，每组穴位针刺时间为30分钟，连续3天。

结果：经过心肌缺血再灌注模型建立后，电针预处理组大鼠的心肌组织出现了较少程度的坏死和炎症浸润，而心肌缺血再灌注组大鼠的心肌组织呈现明显的坏死和炎症反应。在心肌细胞骨架蛋白方面的研究中，电针预处理组大鼠的心肌细胞骨架蛋白表达较对照组和心肌缺血再灌注组有所增加，且电针预处理组中心肌细胞骨架蛋白的表达更为均一和完整。

讨论：心肌缺血再灌注损伤是由多种因素共同作用引起的，其中心肌细胞骨架蛋白的紊乱与损伤密切相关。本研究结果表明，“标本配穴”电针预处理可以通过调节心肌细胞骨架蛋白的表达，减轻心肌缺血再灌注损伤。

结论：本研究结果表明，“标本配穴”电针预处理可以通过调节心肌细胞骨架蛋白的表达，减轻心肌缺血再灌注损伤。这一发现为电针预处理在心血管疾病临床应用提供了新的理论基础，也为心肌缺血再灌注的治疗提供了新的思路。

关键词：标本配穴；电针预处理；心肌缺血再灌注；心肌细胞骨架蛋白；大鼠

Abstract:Myocardialischemia-reperfusioninjuryisacommonandseriouspathophysiologicalprocessoftheheart,andelectroacupuncturepre-treatmentisanadvancedtreatmentmethodthathasbeenwidelyusedinthecardiovascularfield.Theaimofthisstudywastoinvestigatetheinfluenceof"StainlessSteelAcupuncturePoints"electroacupuncturepre-treatmentonthemyocardialcytoskeletonproteininaratmodelofmyocardialischemia-reperfusion.

Methods:FortymaleSDratswererandomlydividedintocontrolgroup,myocardialischemia-reperfusiongroup,andelectroacupuncturepre-treatmentgroup,with10ratsineachgroup.Thecontrolgroupratsdidnotundergoanyoperation,whiletheratsinthemyocardialischemia-reperfusiongroupandelectroacupuncturepre-treatmentgroupweresubjectedtoleftanteriordescendingbranchligationtoprepareamyocardialischemia-reperfusionmodel.Theelectroacupuncturepre-treatmentgroupreceivedelectroacupuncturepre-treatmentbeforethemyocardialischemia-reperfusionsurgery,withafrequencyof10Hz,currentamplitudeof2mA,andadurationof30minutesperacupointfor3consecutivedays.

Results:Aftertheestablishmentofthemyocardialischemia-reperfusionmodel,themyocardialtissueofratsintheelectroacupuncturepre-treatmentgroupshowedlessnecrosisandinflammatoryinfiltration,whilethemyocardialtissueofratsinthemyocardialischemia-reperfusiongroupexhibitedobviousnecrosisandinflammatoryreaction.Intermsofthestudyonmyocardialcytoskeletonprotein,theexpressionofmyocardialcytoskeletonproteinintheelectroacupuncturepre-treatmentgroupwasincreasedcomparedtothecontrolandmyocardialischemia-reperfusiongroups,andtheexpressionofmyocardialcytoskeletonproteinwasmoreuniformandintactintheelectroacupuncturepre-treatmentgroup.

Discussion:Myocardialischemia-reperfusioninjuryiscausedbythecombinedactionofvariousfactors,andthedisorganizationanddamageofmyocardialcytoskeletonproteinarecloselyrelatedtoit.Theresultsofthisstudysuggestthat"StainlessSteelAcupuncturePoints"electroacupuncturepre-treatmentcanreducemyocardialischemia-reperfusioninjurybyregulatingtheexpressionofmyocardialcytoskeletonprotein.

Conclusion:Theresultsofthisstudysuggestthat"StainlessSteelAcupuncturePoints"electroacupuncturepre-treatmentcanreducemyocardialischemia-reperfusioninjurybyregulatingtheexpressionofmyocardialcytoskeletonprotein.Thisfindingprovidesanewtheoreticalbasisfortheclinicalapplicationofelectroacupuncturepre-treatmentincardiovasculardiseasesandalsoprovidesnewideasforthetreatmentofmyocardialischemia-reperfusion.

Keywords:StainlessSteelAcupuncturePoints;electroacupuncturepre-treatment;myocardialischemia-reperfusion;myocardialcytoskeleto