大鼠ELISA试剂盒说明书

大鼠(FA)ELISA试剂盒说明书大鼠(FA)ELISA试剂盒说明书供应商：上海乔羽生物有限公司上海乔羽有限公司，有elisa试剂盒，抗体，培养基大鼠(FA)ELISA试剂盒说明书Liquidtypespecimen:Includingserum,plasma,urine,pleuraleffusionandascites,cerebrospinalfluid,cellculturesupernatant,etc..Serum:Roomtemperaturebloodnaturalcoagulation10-20minutesafterthecentrifugeabout20minutes(2000-3000turn/min).Carefullycollectthesupernatant.Intheprocessofpreservation,ifthereisprecipitation,shouldbere-centrifugal.Plasma):AccordingtotherequirementsofthespecimensofchoiceofEDTA,sodiumcitrateorheparinasananticoagulant,10-20minutesaftermixing,centrifugedfor20minutesorso(2000-3000r.p.m.).Carefullycollectthesupernatant.Intheprocessofpreservation,ifthereisprecipitation,shouldbere-centrifugal.Urine:Collectingwithsteriletubes.Centrifugal20minutesorso(2000-3000turn/min).Carefullycollectthesupernatant.Intheprocessofpreservation,ifthereisprecipitation,shouldbere-centrifugal.Pleuraleffusion,cerebrospinalfluidwithreferencetothispractice.Cellculturesupernatant:Tocollecttheingredientsforthedetectionofsecretorycomponents.Centrifugal20minutesorso(2000-3000turn/min).Carefullycollectthesupernatant.Detectionofcellcomponents,withPBS(PH7.2-7.4)dilutedcellsuspension,thecellconcentrationreached1million/mlorso.Throughrepeatedfreezingandthawing,inordertomakethecelldamageandreleaseofintracellularcomponents.Centrifugal20minutesorso(2000-3000turn/min).Carefullycollectthesupernatant.Intheprocessofpreservation,ifthereisprecipitation,shouldbere-centrifugal.Tissuespecimen:Aftercuttingthespecimen,weightheweight.AddacertainamountofPH7.4,PBS.Rapidfreezingwithliquidnitrogen.Thespecimensweremeltedandstillmaintainedat2-8.AddacertainamountofPBS(PH7.4),withahandorahomogenateofthespecimenhomogenatefull.Centrifugal20minutesorso(2000-3000turn/min).Carefullycollectthesupernatant.Bedetectedafterarepackaging,remainingfrozenspare.大鼠(FA)ELIS弑剂盒说明书ELISAtechnologyprinciple:Basedonimmunologicalreaction,thespecificreactionofantigenandantibodywascombinedwiththehighefficiencyofenzymetosubstrate.solidphaseofantigenorantibodyandenzymemarkerofantigenorantibody.bindingtotheantigenorantibodyonthesurfaceofthesolidsupportstomaintainitsimmunologicalactivity.theenzymelabeledantigenorantibodynotonlyretainsitsimmunologicalactivity,butalsoretainstheactivityoftheenzyme.theantigenorantibodyonthesurfaceofthespecimenandthesolidcarrier.Enzymelabeledantigenorantibodyisthenaddedtothesolidsupport.atthistime,theamountofenzymeinthesolidphaseandtheamountofthesubstanceinthesamplewereinacertainproportion.afteraddingthesubstrateoftheenzymereaction,thesubstrateiscatalyzedbytheenzymetobeacoloredproduct,andtheamountoftheproductisdirectlyrelatedtotheamountofthesubstanceinthesample,sothequalitativeorquantitativeanalysiscanbecarriedoutaccordingtothecolorofthecolor.Determinationofthemethodhasahighsensitivity(pg-ng/mllevel),andgoodreproducibility.MaterialsprovideduillitheklfMateriahprovidedwiththekitnations96detemiinationsStorageUsermanual11Closureplat&m&mbrane22Sealedbags11Microelisastripplate112-8^CShndardi27Mng/L0.5mlx1bottle0.5ml*1bottleStandarddiluent1一5e|m1hottiet顷n网bottio害"HRB-Conjugatereagent3ml^1bottle6rnl*ibotlleA件Samplediuent3伸1bottfebottle2-睥ChfoirtogenSolutionA3niWbottle6ml时bdH&2-SrCChromogenSolutionB3m1^1bottlebotlleStopSolution3m1bottlebotlle2-8"Cwashsolution(20mlX20lold»xibottle(20mlX30fold)xibottle2-sr大鼠(FA)ELISA试剂盒说明书Mattersneedingattention：kitfromthecoldstorageenvironmentshouldberemovedatroomtemperaturefor15-30minutesaftertheuseoftheenzymelabeledpackageisnotusedupaftertheplate,theplateshouldbestoredinasealedbag.washingbufferwillcrystallization,heatedthewatersolubilizationdilution,washingdoesnotaffecttheresults.eachstepsampleshouldbeused,andoftentocheckitsaccuracy,inordertoavoidthetesterror.Asamplewithin5mins,ifthenumberofsampleismuch,recommendtousevolleylike.pleasemakethestandardcurveatthesametime,itisbettertodothehole.Suchassamplestobemeasuredmattercontentistoohigh(thesampleodhighstandardproductthefirstholeholeOD),pleasefirstsampledilutionmultiples(ntimes)weremeasuredandcalculatedpleasethenmultipliedbythetotaldilutionratio(n**5).closureplatemembraneonlyaone-timeuse,toavoidcrosscontamination.substratepleaseavoidlightpreservation.instrictaccordancewiththeinstructionsoftheoperation,theresultsofthetestmustbedeterminedinaccordancewiththeenzymestandardinstrument