孙秋娟(呙于明)：不同来源铜、锌、锰对产蛋鸡的生产性能、蛋品质、酶活及微量沉积的影响

不同来源铜、锌、锰对产蛋鸡的生产性能、蛋品质、酶活及微量沉积的影响

EffectsofDifferentSourcesofCu/Zn/MnonPerformance,EggQuality,EnzymeActivityandMineralRetentioninLayingHens报告人孙秋娟Reporter:SunQiujuan导师呙于明教授Supervisor:ProfessorGuo

Yuming

中国农业大学动物科技学院CollegeofAnimalScience&Technology,ChinaAgriculturalUniversity研究背景Background锌、铜、锰为动物各种生理过程的必要元素。Zinc,copperandmanganeseareessentialinallanimalsforvarietyofphysiologicalprocess（Richards,2007）.调节免疫、组织结构完整、抗氧化、酶的组成等等。Immuneresponse,tissueintegrity,protectingfromoxidativedamage,formationofenzymes,etc.稳定性及拮抗物StabilityandAntagonisms研究背景BackgroundKnownmineralinteractions(Miller,1979)Phytate有机微量来源

OrganicMineralsSources络合物Complexes螯合物Chelates

蛋白盐Proteinates富硒酵母SelenizedYeastMINTREXZn/Cu/Mn为两个蛋氨酸羟基类似物配体与一个金属原子形成的螯合物。MINTREXZn/Cu/Mnisachelateoftwo2-hydroxy-4(methylthio)butanoic

acid(HMTBa)ligandsperatomoftracemineral.ZincAminoAcidchelate氨基酸螯合锌研究背景Background有机微量元素在肉鸡日粮中使用，其生物利用率要高于无机微量元素（Abdallah,2009；Peric,2008；Swiatkiewicz,2008等）。Organicmineralshavehigherbioavailabilityinbroilers.日粮中添加蛋氨酸可提高小鼠对锌的吸收（House,1997）。Methionineinratdietcanaffectzincabsorption.试验目的Objective评定在剔除配体影响后，MintrexCu/Zn/Mn对蛋鸡生产性能、蛋品质及酶活、微量沉积等的影响。

AssesseffectsofdifferentCu/Zn/Mnsources(NovusInternationalInc.)withthesamelevelofmethionineonperformance,eggquality,enzymeacitivityandmineralretentionoflayinghens.材料和方法MaterialsandMethods试验采用315只37周龄海兰褐蛋鸡，随机分成4个处理，每处理6个重复，每重复3笼，每笼3只鸡。Atotalof31537wk-oldHylinebrownlayers,2treatments,6replicatespertreatment(3pensperreplicatewith3layerseach).Treatmentdiet日粮处理处理Cu（ppm）Zn（ppm）Mn（ppm）CuSO4MintrexCuZnSO4MintrexZnMnSO4Mintrex

MnT110-30-30-T2-1030-30-T310-102030-T410-30-1020材料和方法MaterialsandMethods各日粮能量、含氮量及蛋氨酸含量相同。Alldietswereiso-energetic,iso-nitrogenousandiso-methionine,partoftheMetsourcewasprovidedfromMintrex.ZincAminoAcidchelate氨基酸螯合锌

Mintrex

Cu:Cu15%,Met78%;Mintrex

Zn:Zn16%,Met80%;

Mintrex

Mn:Mn13%,Met76%。基础日粮微量含量为：Cu6.5ppm，Mn17ppm，Zn20ppm。Tracemineralsinbasaldiets:Cu6.5ppm,Mn17ppm,Zn20ppm.预饲两周，不添加微量元素。试验期为39周龄到48周龄。Twoweeksforpre-feedingwithnomineralssupplement.Theexperimentlastedfortenweeksfrom39to48wksofage.

测定指标Determination生产性能Production产蛋率、采食量、蛋重、破蛋率Layingpercentage,Feedintake,Eggweight,Crackedpercentage蛋品质EggQuality蛋壳厚度，蛋壳强度，ShellthicknessShellstrength存放10天后的蛋白高度、哈氏单位及蛋黄颜色酶活EnzymesActivityTreatment1&2:CuZn-SOD&Ceruloplasmin

铜锌超氧化物歧化酶、铜蓝蛋白Treatment1&3:Carbonicanhydrase&Alkalinephosphatase&CuZn-SOD

碳酸酐酶、碱性磷酸酶、CuZn-SODTreatment1&4:Catalase、Mn-SOD

过氧化氢酶、Mn-SOD微量沉积MineralRetention肝脏、胰脏、脾脏、胫骨、趾骨Liver,pancreas,spleen,tibia,phalanxStatisticalAnalysis统计分析使用SASv8统计软件，与对照组用TTEST进行显著性检验。AlldatawasanalyzedusingtheSASprogramversion8(SASInstitute).MeansofvariableswerecomparedusingTTEST.试验结果——铜Results-CopperControlMintrexCupvalue39-43wkEggWeight(g)平均蛋重61.3±0.961.5±2.40.8350LayingRate(%)鸡日产蛋率90.4±2.991.5±2.60.4914CrackRate(%)破蛋率0.58±0.860.60±0.760.9591FCR（Feed：EggMass）料蛋比1.91±0.071.87±0.080.448EggMass(g)鸡日产蛋量55.4±2.256.2±1.90.4801FeedIntake(g)采食量105.6±6.3104.3±3.10.670539-48wkEggWeight(g)61.7±0.962.0±2.20.7332Layingrate(%)90.1±0.890.8±0.90.5916CrackRate(%)0.44±0.270.40±0.270.7365FCR1.92±0.081.89±0.140.648EggMass(g)55.7±0.756.3±0.70.5455FeedIntake(g)107.5±6.5108.3±6.50.9459不同铜来源对生产性能的影响EffectsofCusourcesonproduction试验结果——铜Results-Copper不同铜来源对储存10天后蛋品质的影响EffectsofCusourcesoneggqualityafter10daysstorage

ControlMintrexCupvalue蛋黄颜色7.6±0.57.0±0.80.0256蛋白高度4.91±0.885.74±0.830.0132哈氏单位67.2±7.473.9±6.30.0163试验结果——铜Results-CopperControlMintrexCupvalue43周龄16.97±0.7823.39±1.590.007948周龄22.88±2.0734.45±2.100.0682不同铜来源对肝脏铜蓝蛋白（CP）的影响EffectsofCusourcesonCPinliver试验结果——铜Results-Copperp<0.01p<0.10p<0.05p<0.01小结-铜Summary-CuMintrexCu等量代替硫酸铜可提高43周龄蛋黄中、48周龄肝脏及脾脏中铜水平。MintrexCutoreplaceCuSO4increasedCulevelinyolkatwk43,liverandspleenatwk48.铜蓝蛋白活性也随之升高。CP(ceruloplasmin)activitywasincreasedatwk48.减缓储存对蛋品质的不利影响。Slowdowneffectsofstorageoneggquality.试验结果——锌Results-ZincControlMintrexZnpvalue39-43wkEggWeight(g)平均蛋重61.3±0.962.0±1.00.1659LayingRate(%)鸡日产蛋率90.4±2.990.0±2.20.7322CrackRate(%)破蛋率0.58±0.860.44±0.520.5106FCR(Feed：EggMass)料蛋比1.91±0.071.86±0.230.604EggMass(g)鸡日产蛋量55.4±2.255.8±1.20.681FeedIntake(g)采食量105.6±6.3103.4±12.40.689639-48wkEggWeight(g)61.7±0.962.5±0.80.1143Layingrate(%)90.1±0.889.9±0.90.8569CrackRate(%)0.44±0.270.73±0.290.464FCR1.92±0.081.89±0.080.786EggMass(g)55.7±0.756.2±0.80.5182FeedIntake(g)107.5±6.5106.7±10.20.961不同锌来源对生产性能的影响EffectsofZnsourcesonproduction试验结果——锌Results-Zinc不同锌来源对储存10天后蛋品质的影响EffectsofZnsourcesoneggqualityafter10daysstorage

ControlMintrexZnpvalue蛋黄颜色7.6±0.56.9±0.60.0017蛋白高度4.91±0.885.88±0.970.0080哈氏单位67.2±7.473.0±7.30.0438试验结果——锌Results-ZincControlMintrexZnpvalue43周龄0.48±0.090.62±0.070.018648周龄0.70±0.100.92±0.140.0196不同锌来源对肝脏碳酸苷酶（CA）的影响EffectsofZnsourcesonCAactivityinliver试验结果——锌Results-Zincp=0.06p<0.01p<0.01小结-锌Summary-ZincMintrexZn部分替代硫酸锌，43周龄蛋黄锌水平提高，胫骨、脾脏锌水平降低。Znlevelinyolkwereincreased,butdecreasedintibiaandspleenatwk43bypartiallyreplacingZnsulfatewithMintrexZn.肝脏碳酸苷酶活性提高。CAactivitiesinliveratwk48wereenhanced.减缓储存对蛋品质的不利影响。Slowdowneffectsofstorageoneggquality.试验结果——锰Results-ManganeseControlMintrex

Mnpvalue39-43wkEggWeight(g)平均蛋重61.3±0.962.3±2.10.282LayingRate(%)鸡日产蛋率90.4±2.991.3±3.40.6055CrackRate(%)破蛋率0.58±0.860.42±0.530.942EggMass(g)鸡日产蛋量55.4±2.256.9±3.60.388FCR（Feed：EggMass）料蛋比1.91±0.071.71±0.420.248FeedIntake(g)采食量105.6±6.396.4±21.50.401539-48wkEggWeight(g)61.7±0.963.7±2.20.035Layingrate(%)90.1±0.891.3±1.00.4267CrackRate(%)0.44±0.270.64±0.310.9404EggMass(g)55.7±0.757.5±0.90.2158FCR1.92±0.081.73±0.090.326FeedIntake(g)107.5±6.595.2±15.50.4752不同锰来源对生产性能的影响EffectsofMnsourcesonproduction试验结果——锰Results-ManganeseControlMintrex

Mnpvalue43wkShellThickness蛋壳厚度335.2±15.9342.9±20.40.1943ShellStrength蛋壳强度3.00±0.473.18±0.610.243348wkShellThickness334.4±24.0350.5±25.30.0171ShellStrength3.21±0.513.31±0.570.4865p<0.05不同锰来源对蛋壳质量的影响EffectsofMnsourcesonshellquality试验结果——锰Results-Manganese不同锰来源对储存10天后蛋品质的影响EffectsofMnsourcesoneggqualityafter10daysstorage

ControlMintrex

Mnpvalue蛋黄颜色7.6±0.57.0±0.50.0038蛋白高度4.91±0.885.45±0.960.1242哈氏单位67.2±7.470.0±7.70.3303试验结果——锰Results-ManganeseControlMintrex

MnpvalueMn-SOD48.0±6.6108.6±2