小鼠常用实验技术课件

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LaboratoryAnimalHandlingTechnique

-Mouse-Rat-RabbitLaboratoryAnimalHandlingTecObjectiveTocomplywiththeAnimalWelfareOrdinanceandavoidmishandlingofanimalinresearchToprovidebasicconceptsofanimalhandlingtechniquetonewanimaluserWhileofferingourconceptandtechniquestoouranimaluser,wealsoencouragecommentsfromexperiencedanimalusers.Bydoingso,wewouldenrichourknowledgeinthefieldoflaboratoryanimalresearchonbothsidesandfurtherbenefitanimalwelfareaswellasthecredibilityofresearchresultinouruniversityObjectiveTocomplywiththeAnLaboratoryAnimalHandlingTechnique-MouseA.BloodcollectionfromtailveinB.BloodcollectionfromorbitalsinusC.BloodcollectionfromcardiacpunctureD.BloodcollectionfromsaphenousveinE.IntraperitonealinjectionF.SubcutaneousinjectionG.OralFeedingH.SexingLaboratoryAnimalHandlingTecBloodCollectionFromTailinMouseForcollectionofsmallamountofblood(Approximate0.1ml)BloodCollectionFromTailinToolsforBloodCollectionfromTail75%alcoholcottonballforsurfacedisinfectionSmallplasticbottlewith1/2cmdiameterholesinbothendsasmouserestrainerScissorsPipettemanandtipsAvialforbloodcollectionToolsforBloodCollectionfroPlacingamouseonacagelidandgraspingthelooseskinbehindtheearsbythethumbandforefingerPlacingamouseonacagelidPushthemouseintotherestrainerPushthemouseintotherestraLeavethetailofthemouseoutsidethecoveroftherestrainerLeavethetailofthemouseouAmputatethetipofthemousetailbyscissorsAmputatethetipofthemouseMassagethetailandcollectbloodbypipettemanMassagethetailandcollectbBloodCollectionFromOrbitalSinusinMouseShouldapplyanestheticbeforebloodwithdrawAconvenienceandeasyapplymethodforbloodcollectioninmouseCollectamountupto0.5mlBloodCollectionFromOrbitalToolsforBloodCollectionfromOrbitalSinusinMouse75%alcoholcottonballforsurfacedisinfectionHypnormforgeneralanesthetic27Gneedlewith1mlsyringeforinjectionGlasscapillarytubeandvialforbloodcollectionToolsforBloodCollectionfroAnesthetizeamousebyintraperitonealinjectionofHypnormAnesthetizeamousebyintrapeUseasharpendglasscapillarytubetopenetratetheorbitalconjunctivaandrupturetheorbitalsinusUseasharpendglasscapillarCollectbloodwithavialCollectbloodwithavialBloodCollectionFromCardiacPunctureinMouseForcollectupto1mlofbloodwithinashortperiodoftimeMustbeperformedundergeneralanestheticBloodCollectionFromCardiacToolsforCardiacpunctureinMouse75%alcoholcottonballforsurfacedisinfectionHypnormusedasanesthetic27Gneedlewith1mlsyringeforinjection24Gneedlewith3mlsyringeforbloodwithdrawToolsforCardiacpunctureinAnesthetizeamousebyintraperitonealinjectionofHypnormAnesthetizeamousebyintrapeDisinfectthethoraxareawith75%alcoholcottonballDisinfectthethoraxareawithSearchforthemaximumheartpalpitationwithyourfingerSearchforthemaximumheartpInserta24G1”needlethroughthethoracicwallatthepointofmaximumheartpalpitationInserta24G1”needlethroughWithdrawbloodslowlybyyourrighthandWithdrawbloodslowlybyyourBloodCollectionFromSaphenousVeininMouseThismethodisusedofmultiplesamplesaretakeninthecourseofadayItcanalsobeappliedonrats,hamsters,gerbilsandguinea-pigsBloodCollectionFromSaphenouToolsforbloodcollectionfromSaphenousveininmice75%alcoholcottonballforsurfacedisinfection50mlsyringetubewithsmallholesattheendasrestrainerascalpelandshaverforremoveofhair24G1“needleforreleaseofbloodtipsandpipettemanforbloodcollectionToolsforbloodcollectionfroPlacingamouseonacagelidandgraspingthelooseskinbehindtheearswithyourthumbandforefingerPlacingamouseonacagelidPlacethemouseintherestainerPlacethemouseintherestainPulloutthelegandremovedthehairbyaassistantPulloutthelegandremovedtHaircanalsobeshavedbyusingasmallscalpelHaircanalsobeshavedbyusiThesaphenousveinisseenonthesurfaceofthethighThesaphenousveinisseenonApplyvaselineafterdisinfectthesurfaceareatoreduceclottingandcoagulationduringbloodcollection.ApplyvaselineafterdisinfectUsea24G1”needletopuncturetheveinandreleasebloodfromthesaphenousveinUsea24G1”needletopunctuUseaMicrovetteorapipettemanwithtiptocollectbloodfromthesaphenousveinUseaMicrovetteorapipettemApproximate100microliterscanbecollectedApproximate100microliterscaFlexthefootofthemousetoreducetheflowofbloodbacktothepuncturesiteFlexthefootofthemousetoAcottonballisappliedtothepuncturesitetostopfurtherbleedingAcottonballisappliedtothIntraperitonealInjectioninMouseAcommonmethodofadministeringdrugstorodentsIntraperitonealInjectioninMToolsforIntraperitonealInjectioninMouse75%alcoholcottonballforsurfacedisinfection25G1/2”needlewith1mlsyringeforinjectionToolsforIntraperitonealInjPlaceamouseonacagelidandgraspingthelooseskinbehindtheearswithyourthumbandforefingerPlaceamouseonacagelidanAssoonasthemouse’sheadisrestrained,themousecanbepickedupandthetailsecuredwithinyourringfingerandlittlefingerAssoonasthemouse’sheadisTheinjectionsiteshouldbeinthelowerleftquadrantoftheabdomenbecausevitalorgansareabsentfromthisarea.

Onlythetipoftheneedleshouldpenetratetheabdominalwalltopreventinjectionintotheintestine.TheinjectionsiteshouldbeiSubcutaneousInjectioninMouseThemostcommonmethodforimmunologystudiesSubcutaneousInjectioninMousToolsforSubcutaneousInjectioninMouse75%alcoholcottonballforsurfacedisinfection25G1“needlewith1mlsyringeforinjectionToolsforSubcutaneousInjectiPickupanudemouseandspinit’stailtoputitinafaintconditionPickupanudemouseandspinGraspthelooseskinonthebackofthemousefromearsalongthelegsandrestrainthelegswithyourringfingerandlittlefingerGraspthelooseskinonthebaAfterdisinfectthesurfacearea,inserttheneedleinthelateralsideoftheabdominalwallandpushupwardstothearmpitofthemouseAfterdisinfectthesurfacearInjectthesubstanceslowlyInjectthesubstanceslowlyAlumpofinjectionsubstancecanbeseenthroughtheskinafterinjectionAlumpofinjectionsubstanceOralFeedinginMouseGastricintubationensuresthatallthematerialwasadministeredFeedingamountlimitedto1%ofbodyweightOralFeedinginMouseGastriciToolsforOralFeedinginMouseA18Gstainlesssteel,balltippedneedleagloveToolsforOralFeedinginMousGraspthelooseskinonthebackofthemouseandrestrainit’stailwithyourringfingerandlittlefinger.Then,introducethefeedingtubefromthepharynxintotheesophaguswhenthemouseisintheactofswallowing.GraspthelooseskinonthebaCommoncomplicationsassociatedwithgastricintubationaredamagetotheesophagusandadministrationofsubstanceintothetrachea.Carefulandgentlepassageofthefeedingneedlewillgreatlyreducethesepossibilities.CommoncomplicationsassociateTheanatomypictureshowedthepositionofthefeedingneedletipinsidetheesophaguswiththeheartandsternumremoved.TheanatomypictureshowedtheSexingmice-Thedistancebetweentheanalandgenitalorificesisgreaterinthemale(left)comparedtothefemale(right).Sexingmice-ThedistancebetLaboratoryAnimalHandlingTechnique

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小鼠常用实验技术课件

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