污水生物处理技术活性污泥法

1、Escherichia coli to decompose polluted water and sludgeBy 刘琰，肖琦，邱思，金鑫2008.3.2Background:污水生物处理技术活性污泥法剩余污泥的处理问题 筛选能特异性降解的微生物 提高微生物的降解效率氧化磷酸化解偶联：解偶联是指将呼吸链的氧化作用与磷酸化作用分开的现象。在代谢物的氧化过程中ATP不产生或产生量减少，剩余能量以热能的形式放出。在解偶联的情况下，微生物为了获得满足生长需求的能量，势必会增大对营养物质的代谢消耗。chemical uncoupling VS biological uncoupling Biologic

2、al uncoupling:1) Change components of the respiratory chain of E.coli2) Change proton gradient3) Mutate the F1F0 ATP synthase 1) Respiratory chain of E. coliDifferent dehydrogenases (NADH dehydrogenases ,)Different teminal oxidases or reductases (Quinol oxidases bo3-type & bd-type)Linked by quinones

3、 (Ubiquinone)NADHNDH 1 (nuo)2 H+/e-NDH 2 (ndh)0 H+/e-Quinonebo-type oxidase (cyo)2 H+/e-bd-type oxidase (cyd)1 H+/e-O2Fig1. A schematic representation of several major components of the aerobic respiratory chain of E.coli2)Change proton gradientUncoupling protein(UCP)主要存在于动物及一些高等植物中。当质子从UCP返回膜内而不经过A

4、TP synthase 时，就发生解偶联。How to express UCP1 in E. coli?Signal peptideAvoid of aggregationActivator: free fatty acid?Cofactor: CoQ?3) Mutate ATP synthaseUncouple the ATP synthesis and proton transportTarget sites: , etcS174L: decrease enzyme activity but remain the coupling to proton translocation. can grow by oxidative phosphorylationSummary1、敲除 NDH 1, bo-type oxidase 以