Deoxycholic-acid-sodium-salt-Sodium-deoxycholate-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEDeoxycholic acid sodium saltCat. No.: HY-N0593ACAS No.: 302-95-4Synonyms: Sodium deoxycholate分式: CHNaO分量: 414.55作靶点: GPCR19作通路: GPCR/G Protein储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO :

2、 6 mg/mL (14.47 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.4123 mL 12.0613 mL 24.1225 mL5 mM 0.4825 mL 2.4123 mL 4.8245 mL10 mM 0.2412 mL 1.2061 mL 2.4123 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Deoxycholic acid sodium salt 激活 G 蛋偶联胆汁酸受体TGR5

3、。IC50 & Target TGR5 1体外研究Deoxycholic acid (DCA) and chenoDeoxycholic acid (CDCA), as the common ingredients of duodenal reflux,act synergistically in many physiological and pathological processes. The cells are repeatedly exposed to1/2 Master of Small Molecules 您边的抑制剂师www.MedChemE100 M CDCA and Deox

4、ycholic acid at pH 5.5 for up to 120 min. To simulate chronic local recurrent diseasein vitro, the gastric cancer cell line MGC803 is exposed to acidified medium (pH 5.5) containing 100 MDeoxycholic acid and CDCA. An untreated log-growth MGC803 cell line is generated to be used as a controlin normal

5、 pH media. After daily 10 min exposure to the acidified bile acids for 60 weeks, MGC803-resistantcells are able to survive and proliferate after 120 min exposure 2.PROTOCOLCell Assay 2 MGC803 cells are cultured in Roswell Park Memorial Institute media supplemented with 10% fetal calf serumand 100 U/

6、mL Penicillin and 100 mg/mL Streptomycin. To generate MGC803-resistant cells, the pH value ofthe MGC803 culture medium is adjusted to the experimental conditions using the hydrochloric acid (A). Thebile acids GCDA and Deoxycholic acid are diluted to optimal working concentrations of 100 M (B) withcu

7、lture medium, and the overall pH (A+B) is adjusted to pH 5.5, simulating the gastric environment. Initially,MGC803 cells are chronically exposed to acidified medium with bile acids (A+B) for 10 min every 24 h. Theexperimental time and conditions are optimized in our preliminary experiments, which sh

8、ow that 10 min isenough and does not result in cell damage. This procedure is repeated and it takes 60 weeks for theMGC803 cells to survive and proliferate under the exposure of A+B for 120 min. Control untreated cells arecultured in neutral RPMI medium at pH 7.4 in parallel to the resistant cells f

9、or 60 weeks. The morphologicalchanges in MGC803 cells exposed to acidified bile acids (A+B) are documented at 30 and 60 weeks 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Cell Res. 2019 Mar;29(3):193-205. Nat Cell Biol. 2018 Oct;20(10):

10、1145-1158. Cancer Cell Int. 2019 Jan 31;19:24. Laurea Magistrale in Biomedical Engineering, Politecnico di Milano. 2019 Jun. bioRxiv. 2019 Jan.See more customer validations on HYPERLINK / www.MedChemEREFERENCES1. Somm E, et al. -Klotho deficiency protects against obesity through a crosstalk between

11、liver, microbiota, and brown adipose tissue. JCIInsight. 2017 Apr 20;2(8). pii: 91809.2. Wang X, et al. Acidified bile acids enhance tumor progression and telomerase activity of gastric cancer in micedependent on c-Mycexpression. Cancer Med. 2017 Apr;6(4):788-797.McePdfHeightCaution: Product has not be