DynamicLightScatteringMeasuringPlateletQualityandBacterial动态光散射测量血小板质量和细菌实用教案

1、1 of 17Platelet transfusions used to treat and/or prevent bleedingNumber of platelet transfusions produced per year: Canada: 300,000United States: 2,000,000Europe: 2,500,000Platelets are sensitive to stress (e.g., cold damage) Activated platelets do not help patients.Storage at 22C to prevent cold d

2、amageIncreased risk for bacterial growthShortages - addressed by increasing donor recruitment第1页/共17页第一页，共18页。2 of 17The ProblemClinical Situation Solving the problem Novel ApproachData AcquisitionDLS-PM score The InventionTechnology BackgroundThe DLS-PMAccomplishmentsClinical Results Summary Other

3、Technology Accuracy, Sensitivity第2页/共17页第二页，共18页。3 of 17No non-invasive in vitro test available Tested inventory cannot be transfused Platelet quality / bacterial testing cannot be mandatedGood outcome Assumption: good plateletsBad outcome Assumption: bad plateletsTransfusion outcome serves as retro

4、spective platelet quality indicator. 第3页/共17页第三页，共18页。4 of 17 at least 20% discard rate Additional 10% discarded after testing (invasive technology) Unknown quality of transfused platelets $1,000 / transfusion Worldwide loss of at least 1 billion dollars per yearIn summary: Unknown platelet quality

5、is a safety issue Discarded platelet transfusions are a financial burden第4页/共17页第四页，共18页。5 of 17Multi-dimensional quality measurementDLS-PM detects discoid platelets activated platelets microparticles bacteria at the same time第5页/共17页第五页，共18页。6 of 17Platelets50-100 nm1200 nm BACTERIA (if present)500

6、 nmMicroparticlesTransfus Med Rev 2007; 21(4):295第6页/共17页第六页，共18页。7 of 17Most fresh platelet transfusionsMost platelet transfusions until day 7 - 12 of storageTemperatureResponseMicroparticlesPlateletSize第7页/共17页第七页，共18页。8 of 17 Non-invasive Small sample volume Fast InexpensivePhys Med Biol 2006; 51

7、:3747第8页/共17页第八页，共18页。9 of 17May - Aug 2007First clinical pilot study2006ValidationLatex beadsPlateletsBacteriaProteinsNanoparticlesSept 2005First generation instrumentApril 2005First prototypeNov 2003 Proof of principle第9页/共17页第九页，共18页。10 of 17DLS-PM score vs. transfusion merit第10页/共17页第十页，共18页。11

8、of 17104/160 transfusions65 %Transfusion meritcorrelates with DLS-PM score 第11页/共17页第十一页，共18页。12 of 17 INVASIVE No single instrument can determine platelet quality and function in multiple dimensions Impedance Optical Immunofluorescence Hematology analyzers cannot assess bacterial contamination BacT

9、/Alert requiredSysmex XE-2100 KobeAdvia 120Siemens FacsCanto IIBDBacT/AlertbioMrieux第12页/共17页第十二页，共18页。13 of 170204060801001201401601802001234Without beadsWith beadsBeads第13页/共17页第十三页，共18页。14 of 17Optical methods - Low sensitivity Especially for platelet counts 15,000 / LSegal et al. BrJ Haematol 20

10、05; 128:520.第14页/共17页第十四页，共18页。15 of 17v Mandated technology for hospitals / blood banksv 10,000 hospitals / blood banks in North Americav Extended shelf life - reduced outdate-discard ratev Patient safetyv Monitor manufacturing processesDLS-PM: The only technology for noninvasive bacterial detectio

11、n and platelet quality testing at the same time第15页/共17页第十五页，共18页。Thank You!Keddie Brown, iCaptureAudrey Labrie, CBSDr. Mark Scott, CBSDr. Kate Chipperfield, VGHCheryl Pittendreigh, CBSNobu Kitamura, CBSCBS Intellectual Property, Intramural and Bridge Funding第16页/共17页第十六页，共18页。17 of 17感谢您的观看(gunkn)！第17页/共17页第十七页，共18页。NoImage内容(nirng)总结1 of 17。T