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1、微生物培养基大全(国外翻译版)146 种培养基配方供参考。146 种培养基配方THECOMPOSITIONOFMEDIA培养基及成分I.AcetobacterMedium(醋酸菌培养基)Glucose(ffi 萄糖)100gYeasstextract(孝母膏)10gCaCO320gAgar(xx)15gDistilledwater(蒸储水)1000mlAdjust(调)pHto6.8适用范围:恶臭醋酸杆菌混浊变种2.NutrientAgar(营养肉汁琼脂)Pepton(蛋白月东)5gBeefextract(牛肉膏)30gNaCl5gAgar(xx)15gDistilledwater(蒸储水)A

2、djust(调)pHto7.07.2Note:WhencultivationofBacillus, 5mgoftoMnSO5 / 654.H2Omaybeadded .Itisfavorabletopromotesporeformation .适用范围:产气气杆菌、粪产碱杆菌、蜡状芽孢杆菌、蜡状芽孢杆菌蕈状变种、地衣形芽孢杆菌、巨大芽孢杆菌、多粘芽孢杆菌、尘埃芽孢杆菌、短小芽孢杆菌、嗜热脂肪芽孢杆菌、枯草芽孢杆菌、枯草芽孢杆菌深黑变种、苏云金芽孢杆菌、苏云金芽孢杆菌蜡螟亚种(青虫菌)、苏云金芽孢杆菌戈尔斯德变种、苏云金芽孢杆菌猝倒亚种、产氨短杆菌、黄色短杆菌、谷氨酸棒状杆菌、北京棒杆菌、大肠埃

3、希氏菌(大肠杆菌)、铜绿假单胞菌(绿脓杆菌)、凸形假单胞杆菌、荧光假单胞菌、弯曲假单胞菌、恶臭假单胞菌、假单胞杆菌、藤黄八叠球菌、亚黄八叠球菌、尿素八叠球菌、金黄色葡萄球菌、运动发酵单孢菌3.AzotobacterMedium(固氮菌培养基)KH2PO40.2gK2HPO40.8gMgSO4.7H2O0.2gCaSO4.2H2O0.1gNa2MoO4.2H2OTrace微量)Yeastaxtract既孝母膏)0.5gMannitol(甘露醇)20gFeCl3Tract量)Distilledwater(蒸储水)1000mlAgar密月旨)15gAdjust(调)pHto7.2适用范围:固氮菌、胶

4、质芽孢杆菌4 .CornMealMedium(玉米粉培养基)Maizeflour(玉米粉)5gPeptone(蛋白月东)0.1gGlucose(ffi 萄糖)1gTapwater(自来水)1000mlNote:Boilthemixtureinautoclaveat121 C for1hr.distributethemediuminto18 x 18mmtu bes,eachcontains10mloftheliquid,thenautoclaveat121 for1hr .again(15 磅蒸煮 1 小 时,分装入18 x 18毫米试管,每管深度达6厘米。15磅再次灭菌1小时。)5 .Lac

5、tic-bacteriaMediumI(乳酸菌培养基 I)Yeastextract(酵母膏)7.5gPeptone(蛋白月东)7.5gGlucose(ffi 萄糖)10gKH2P042gTomatojuice(西红柿汁)100mlTween(吐温)800.5mlDistilledwater( 蒸馏水 )900mlpH7.0适用范围:植物乳杆菌(胚芽乳杆菌)、嗜热乳酸链球菌6 .Lactic-bacteriaMidium H (乳酸菌培养基 H )Lacto-caseinpeptone俘L酪蛋白月东)10gBeefextract(蛋白月东)10gYeastextract(酵母膏)5gGlucos

6、e葡萄糖)5gTween(吐温)801gK2HPO42gNa-acetate(醋酸钠)5gDiaminecitrate(柠檬酸二胺)2gMgSO4.7H2O0.2gMnSO4.H2O0.05gDistilledwater( 蒸馏水 )1000mpH6.5 6.8适用范围:植物乳杆菌(胚芽乳杆菌)7.PeotoneGlucoseYeastextractMediumPGY(蛋白月东、酵母膏、葡萄糖培养 基)Peptone (蛋白月东)10gYeastextract (酵母膏)5gGlucose (葡萄糖)IgDistilledwater (蒸储水)1L1.1 lycerolAgar (甘油琼脂)P

7、eptone (蛋白月东)5gBeefextract (酵母膏)3gGlycerol (甘油)20gTopwater (自来水)1000mlAgar( xx) 15gpH1.2 1.39.Rhizobiummedium (根瘤菌培养基)AS9Yeasteztract (酵母膏)1gSoileztract (土壤浸提液)200mlMannitol (甘露醇)10gAgar (琼脂)15gDistilledwater (蒸储水)800mlpH7.2Note:Soilextract:Suspend50gfinelyanddriedgardonsoilin200mloftapwater.Autocla

8、veat121 for1h r.Decantthroughcotton- cloth,fillerthoughpaper,makeupvolumeto200ml.Resterilizefor20minutesat121 ,then mixedwithothringredientsanddistributed.( 土壤浸提液的制法:取土壤 50 克,加水200 毫升, 15 磅蒸煮 1 小时,经滤纸过滤后加水补足到200 毫升。)适用范围:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根 瘤菌1.1 Mannitol

9、Agar(甘露醇琼月旨)Yeastextract(酵母膏)5gPeptone(蛋白月东)3gMannitol(甘露醇)25gAgar晾脂)15gDistilledwater( 蒸馏水)1000ml1.2 GlucoseAsparagine (葡萄糖、天门冬素琼脂)Glucose (葡萄糖)10gAsparagine (天门冬素)0.5gK2HPO40.5 gWater (水)1000mlpH40.640.7适用范围:刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌)12.Gause ' sSyntheticAgaU氏合成一号琼脂)KNO31gSolublestarch (可溶性淀粉)2

10、0gK2HPO40.5gMgSO4.7H2O0.5gNaCl0.5gFeSO7 / 6540.01gAgar (琼月旨)20gwater (水)1000mlpH1 .22 .4适用范围:刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌)、白黄链霉菌、白色链霉菌、抗生链霉菌、双重轮丝链霉菌、产色链霉菌、烬灰链霉菌、天蓝 色链霉菌、灭蚊链霉菌、红霉素链霉菌、青色链霉菌、球孢链霉菌、浅灰链霉 菌、灰色链霉菌、吸水链霉菌、淡紫灰链霉菌、黄色长孢链霉菌、藤黄色链霉 菌、细黄链霉菌、黑化链霉菌、玫瑰色链霉菌、华美链霉菌、嗜热链霉菌、委内瑞拉链霉菌、紫色直丝链霉菌、紫色链霉菌、绿色链霉菌13 .WortAg

11、ar (xx 汁 xx)Dilutetheworld(withouthop)to12Brix.Add15garinto1000mlofthedilutedword.M elttheagarbyheating,thendistributethemediumintotubes.Autoclaveat110for30minute s.(将发酵啤酒的原料(未加酒花),稀释至 12柏林,加琼脂15克,溶化后分 装。 15 磅灭菌 30 分钟。)适用范围:克鲁斯假丝酵母、郎比可假丝酵母、解脂假丝酵母、马其顿假丝酵母、拟热带假丝酵母、粗壮假丝酵母、皱褶假丝酵母、热带假丝酵母、产朊假丝酵 母、阿舒假囊酵母、白

12、地霉、果香地霉、地霉属、异常汉逊酵母、异常汉逊酵 母变种、阿拉伯糖醇汉逊酵母、施氏汉逊酵母、菅囊酵母、伯顿毕赤酵母、膜 醭毕赤酵母、粘红酵母、小红酵母、胶红酵母、深红酵母、卡尔斯伯酵母、酿 酒酵母、椰园酿酒酵母、发酵性酵母、洛格酵母、罗斯酵母、鲁氏酵母多形鲁 氏酵母、威尔酵母、酵母、路德类酵母、栗酒裂殖酵母、掷抱酵母、贝雷丝孢 酵母、皮状丝孢酵母、糙孢曲霉、无壳曲霉、鲜橙曲霉、红曲霉、紫红曲霉、 红色红曲霉、红曲霉菌14 .PotatoDextroseAgarPDA马铃薯、葡萄糖琼脂)9 / 65Potatoextract(马铃薯汁)1000mlDextrose(glucose)葡萄糖)20g

13、Agar(xx)20gNote:Potatoextract:Slicepotatoesthin.Add1000mlofwater,immediatelytopreventOxidtionofjuiceand boiluntilsoft.(approximately30min.).Filterthroughcotton- cloth.Autoclaveat115 for20minutes. (注:取去皮马铃薯200 克,切成小块,加水1000 毫升煮沸30 分钟,滤去马铃薯块,将滤液补足至1000毫升,加葡萄糖20克,琼脂15克,溶化后分装,15磅灭菌 30 分钟。)适用范围:酵米面假单胞菌(酵

14、米面黄杆菌)、白色链霉菌、烬灰链霉菌、青色链霉菌、球孢链霉菌、灰色链霉菌、龟裂链霉菌、伞枝梨头霉、雅致放射毛 霉、棒曲霉、米曲霉、出芽短梗霉、白僵霉、灰葡萄孢、顶头孢霉、巴西毛壳、长刺毛 壳、橄榄包毛壳、反曲毛壳、琥珀毛壳、圆酵毛壳、束状刺盘孢、新月弯孢霉、奇异 翅孢壳、地生翅孢壳、串珠镰孢、尖镰孢、盘长孢菌(鲁保一号)、银白杨盘长孢、 玉蜀黍长蠕孢、深黄被孢霉、小被孢霉、多头被孢、拉曼被孢霉、葡萄色被孢霉、生 香毛霉、卷枝毛霉、两型孢毛霉、直立毛霉、球孢毛霉、丝球毛霉、大毛霉、多型孢 毛霉、总状毛霉、鲁氏毛霉、五通桥毛霉、黑球漆斑菌、露湿漆斑菌、玫烟色拟青 霉、棉铃虫拟青霉、拟青霉、球形阜孢

15、、白腐菌、少根根霉、华根霉、科恩根霉、戴尔根霉、日本根霉、爪哇根霉、米根霉、点头根霉、绿穗霉、簇抱匍柄霉、雅致 枝霉、康宁木霉、绿色木霉、黄萎轮枝孢、大丽花轮枝孢15 .Czapek ' sAg嘉氏琼脂)Sucrose (蔗糖)30gNaNO33gMgSO4.7H2O0.5gKCl0.5gFeSO4.7H2O0.01gK2HPO41gAgar侬月旨)13gDistilledwater(蒸储水)1000mlpH16 2适用范围:红霉素链霉菌、洋葱曲霉、金头曲霉、泡盛曲霉、泡盛曲霉烟色变种、亮白曲霉、炭黑曲霉、鹿皮色曲霉、棒曲霉、无花果曲霉、黄柄曲霉、黄曲霉、烟曲霉、构巢曲霉、黑曲霉、赭曲

16、霉、米曲霉、寄生曲霉、酱油曲霉、土曲霉、宇佐美曲霉、焦曲霉、杂色曲霉、温特曲霉、阿达青霉、产黄青霉、黄绿青霉、桔青霉、顶青霉、园弧青霉、绳状青霉、灰黄青霉、岛青霉、淡紫青霉、特异青霉、赭绿青霉、产紫青霉、娄地青霉、小刺青霉、荨麻青霉、16.ConcentratedSucroseCzapek 浓糖Ss(i琼月旨)Sucrose 糖)200gNaNO33gMgSO4.7H2O0.5gKCl0.5gFeSO4.4H2O0.01gK2HPO41gAgar侬月旨)13gDistilledwater(蒸储水)1000ml适用范围:xx曲霉17.SyntheticPotatoMedium(综合马铃薯培养基)

17、20%Potatoextract(马铃薯汁)1LGlucose葡萄糖)20gKH2PO43gMgSO4.7H2O1.5gVitanibB1(硫胺素)Trace微量)Agar侬脂)15gpH6适用范围:米曲霉、酱油曲霉、栖土曲霉、宇佐美曲霉、出芽短梗霉、白僵霉、灰葡萄孢、顶头孢霉、巴西毛客、长刺毛壳、球毛壳、橄榄包毛壳、反曲毛壳、琥珀毛壳、圆酵毛壳、多主枝孢、束状刺盘孢、葫芦科刺盘孢、新月弯孢霉、奇11 / 65异翅孢壳、地生翅孢壳、串珠镰孢、蚀脉镰孢霉、盘长孢菌(鲁保一号)、银白杨盘长孢、瓶毛壳、金龟子绿僵菌、生香毛霉、卷枝毛霉、两型孢毛霉、直立毛霉、球孢毛霉、丝球毛霉、大毛霉、多型孢毛霉、总

18、状毛霉、鲁氏毛霉、五通桥毛霉、黑球漆斑菌、露湿漆斑菌、玫烟色拟青霉、棉铃虫拟青霉、淡紫色拟青霉、拟青霉、白腐菌、少根根霉、根霉属的菌、华根霉、科恩根霉、戴尔根霉、台湾根霉、日本根霉、爪哇根霉、黑根霉、雪白根霉、少根根霉、米根霉、点头根霉、三宝垄根霉、根霉属的种、绿穗霉、康宁木霉、木素木霉、拟康氏木霉、木霉、粉红木霉、绿色木霉、黄萎轮枝孢、大丽花轮枝孢、双孢蘑菇、大肥菇、发光假蜜环菌、木耳、皱木耳、毛木耳、大秃马勃、长根菇、金针菇、棘托竹荪、短裙竹荪、短裙竹荪东北变种(暂定)、长裙竹荪、白鬼笔、无裙竹荪、宽鳞大孔菌、凸圆灵芝(白芝)、紫芝、灵芝(红芝,赤芝,日本灵芝,韩国灵芝)、灰树花、猴头菌、

19、香菇、粗毛斗菇、洁丽香菇、豹皮菇、玉蕈、真姬菇、安络小皮伞菌、鬼毛针、黑柄皮伞菌、尖顶羊肚菌、圆锥羊肚菌、高羊肚菌、黑脉羊肚菌、羊肚菌、滑菇、光帽黄伞、尖鳞黄伞、金顶侧耳、榆黄磨、金顶磨、白黄侧耳、紫抱平菇、姬菇、裂皮侧耳、拟囊体仰耳 (鲍鱼菇)、真线仰耳短抱变种、真线侧耳、扇形平菇、扁形平菇、佛罗里达无抱子仰耳、佛罗里达仰耳、蛭菇、糙皮仰耳、平菇、贝形侧耳、鹰翅菌、肺 形侧耳、柳树菌、树窝、扁柄仰耳、美味仰耳、紫抱仰耳、粉褶仰耳、粉红侧 耳、凤尾菇、漏斗状侧耳、榆干仰耳、大榆磨、仰耳、茯苓、黑菇、烟色红 菇、裂褶菌、鸡纵、金耳、黄木耳、银耳、白木耳、紫晶口磨、银丝菇、丝盖 小包脚菇、草菇18

20、 .CornMealMedium(玉米粉培养基)Mixcornmealwithwater20:100(W/V)well .Distributethemixtureinto100mlbottle s,eachbottlecontainsabout30mlofthemixture .Autoclaveat121 for30minutes .(20% 玉米粉装瓶,15磅灭菌30 分钟。 )19 .FilterPaperMedium (滤纸培养基)( NH4) 2SO41gKH2PO41gMgSO0.7gNaCl0.5gDistilledwater (蒸储水)1000mlAstripofilterpa

21、per (滤纸条)6 x1cm (厘 米)20.SoybeanCakeMealAga遁豆饼粉琼月旨)10%infusionofsoybeancakemeal(10%|豆饼粉浸汁)1000mlGlucose(SJ萄糖)10gNaCl2.5g21. Sawdust, Wheat-bran Medium (木屑、麸皮培养基)Sawdust of broad leaf tree 75g Wheat bran 25gPreparation:Mix these two ingredients well . Add appropriate water and blend (the amount of wat

22、eradded may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick ahole on the filling, put the cotton pulg in bottle mouth .壳斗科植物或阔叶树的木屑75 克麸皮 25 克制法:加水适量充分混匀,水量以手捏时指间有水珠为宜,装瓶并压紧,在中心处扎一小洞,堵上棉塞,20 磅灭菌 1 小时。22. Pine Block or Pine Sowdust Medium (松木条或松木屑培养基)1. Cut

23、 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbingsuger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbsfor 1 hr . (W 10 x 2 x 1 厘米的松木条 浸泡在 1-2%的蔗糖水中。使木条吸足糖水,装瓶不宜过紧,用纸包好,20 磅灭菌1 小时。 )2. Pine Sawdust

24、MediumPine Sawdust 松木屑)75% Rice bran 维糠)23-24%Socrose 蔗糖)1-2% Water (appropriate)水(适量)Autoclave at 20 lbs for 1 hr.(20 磅灭菌 1 小时)23. Rice Straw. Dung Medium 稻草、马粪培养基()Dried horse dung (or cow dung)(干马粪或牛粪) 65%Died straw (干稻草)35% Urea (尿素)0.5%Calcium superhosphate (过磷酸钙)0.7% Gypsum powder (石膏粉)1%Ammon

25、ium sulphate (硫酸镂)0.3%Note:Add appropriate water, pile them up and cause them fermentation. (水适量 进行堆积发酵)24. Straw Rice Bran Medium (稻草、米糠培养基)Dried straw (干稻草)75-80% Rice bran (米糠)20-25%Note:Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12hr. Allow the stra

26、w fully (the amount of water added may be decided by kneading with fingers. It is rightwhen a drop of water appeared between fingers). ( 注 :选无霉稻草,切成长约3 厘米的小段,用清水浸泡12 小时,使充分吸水,然后倾去水,加米糠和水,水量以用手捏时指间有水珠为宜。)25. Rice Medium (米饭培养基)Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoc

27、lave at 121c for 30 minutes (orf川 2g of rice into a 15 x 150mm tube). Add 6 ml of water. Autoclave at 121 for 30 minutes. (取大米10克,入 50毫升椎形瓶中,水20-30毫升于15磅灭菌30分钟即成,或取大米2克,装入15x150毫米试管中,加 水 6 毫升,于15 磅灭菌 30 分钟。 )26. Filter Paper Medium (滤纸条培养基)(NH4)2SO4 1g KH2PO4 1gMgSO4.7H2O0.5g Yeast Extract孝母膏)0.1gDi

28、stilled Water (蒸馏水 ) 1000mlStrip of filter paper 1 x 7 cm (滤纸条 1 x 7 厘米)Note:Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml ofthe broth). Autoclave at 121 for 30 minutes. Then put one or two sterilized strips of filter paper into atubeaseptically.( 将上述

29、溶液分配成溶液后 分装入试管,每管1 毫升, 15 磅灭菌 30 分钟。再将灭菌的滤纸条(1 条或 2条)投入试管中即好使用。)27. Yeast Extract-Medium Extract Agar I醉母膏、麦芽膏琼脂)Yeast extract (酵母膏) 10g Mall extract (麦芽膏) 10gGlucose (葡萄糖) 4g Distilled water (蒸馏水) 1000mlAgar (xx) 20g pH7.0适用范围:红霉素链霉菌28. Yeast Extract Peptone(酵母膏、蛋白月东琼脂)Yeast extract (酵母膏)1g Multi-p

30、eptone (多蛋白月东)2gBeef extract (牛肉膏)1g Glucose(葡萄糖)10gAgar (琼月旨)20g Distilled water (蒸储水)1LpH7.029. Glucose Yeast Extract Starch Agar葡萄糖、酵母、淀粉琼脂)Glucose (葡萄糖)10g Yeast extract (酵母膏)10gStarch (淀粉)10g NaCl 5gCaCO3 3g Agar(琼脂)20gDistilled water (蒸储水)1L pH7.030. Wheat Bran Medium (秋皮培养基)Wheat bran (秋皮)36g

31、 (NH4) 2HPO4 10gK2HPO40.2g MgSO4。 7H2O0.1gAgar (琼月旨)20g Distilled water (蒸储水)1LpH7.031. Yeast Sporulation Medium (酵母菌产生子囊孢子培养基)(1) Kleyn MediumKH2PO40.012% K2HPO40.02%Sodium acetate (醋酸钠)0.5% Glucose 葡萄糖()0.062%NaCl0.062% Peptone (蛋白胨)0.25%Biotin (生物素)2 g% Ag琼脂)2%Trace element solution (混合盐类) 1ml/10

32、0mlNote:Trace element solution (混合盐类溶液):MgSO4.7H2O0.4%, NaCl0.4%, CuSO4.5H2O0.002%,MnSO4.4H2O0.2%, FeSO4.4H2O0.2%(2) Gorodkowa MediumGlucose (葡萄糖)0.1% Peptone (蛋白胨) 1%NaCl0.5% Agar (xx) 2%(3) McClary MediumGlucose (葡萄糖)0.1% KCl0.18%Yeast juice (酵母汁)0.25% Sodium actate 醋酸钠()0.82%Agar (xx)1.5%32. BPY

33、Medium (BPYg 养基)Beef extract (牛肉膏) 5g Prptone (蛋白胨) 10gNaCl 5g Yeast extract 酵母膏 () 5gGlucose (葡萄糖) 5g Agar (琼脂) 1-2%Distilled (蒸馏水 ) 1000ml pH7.0适用范围:大肠xx菌(大肠杆菌)、恶臭假单胞菌33. LB Medium (LB培养基)Yeast extact (酵母膏) 5g Peptone (蛋白胨) 10gNaCl 10g Agar 琼脂() 1-2%Distilled water (蒸馏水) 1000ml pH7.0适用范围:大肠xx菌(大肠杆

34、菌)34. Penssay Broth (Penssayfe)汤)Beef extract (牛肉膏)1.5g Yeast extract 酵母膏()1.5gTryptone (胰蛋白胨) 5g NaCl3.5gGlucose (葡萄糖) 1g K2HPO4.3H2O4.8gDistilled water (蒸馏水 ) 1000ml pH7.235. Blond Agar (血琼脂培养基)Peptone (蛋白胨) 10g Glucose 葡萄糖() 2gNaCl 5g NaH2PO42.5gSodium citrate (柠檬酸钠) 5g Brain infusion (脑浸汁) 800ml

35、Pig heart infusion (猪心浸) 200ml Sleep Blood 羊血() 10%pH36.Note:Preparation of pig brain and pig heart infusion:( 1) Smash up 100g fresh pig hear(t without fat and blood vessel) .Add distilled water up to 1000ml;( 2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;( 3)

36、 Keep the two infusions at 50 for 1 hr , then boil for 15 minutes;( 4) Cool and filtrate through gauze . 注 猪心和猪脑浸汁法:( 1)新鲜猪心除去心头和脂肪,搅碎100 克加蒸馏水至1000 毫升;( 2)新鲜猪脑除去血管,取200 克加蒸馏水至1000 毫升;( 3) 50 保温 1 小时,然后煮开15 分钟;( 4)冷却后用纱布过滤备用。NaCl 6g MgSO4.7H2O0.1gKH2PO40.5g CaCl20.1gK2HPO42.0g Yeast extract 酵母膏()1.0

37、g(NH4)2SO42.0g Distilled water (蒸馏水) 1000mlFilter paper strip:one strip (滤纸条:一片 ) pH7.07.4Note:Carboxymethyl Cellulose 2% Agar 2%(斜面培养基去滤纸条用 .2%琼脂及2%羧甲基纤维素钠CMCNa)37. Clostridium Pasreurianum Synthetic Medium (巴氏梭状芽胞杆菌合成培养 基)Glucose (葡萄糖)1% MnSO40.001%KH2PO40.05% FeSO40.001%K2HPO40.05% Yeast extract

38、(酵母膏)0.1%MgSO4.7H2O0.02% Peptone (蛋白胨)0.01%NaCl0.001% CaCO30.5%Distille water (蒸馏水) 100% pH7.038. Yeast Extract-Medium Extract AgaE (酵母膏、麦芽膏琼脂 U )Yeast extract (酵母膏)4g Mall extract (麦芽膏)10gGlucose (葡萄糖)4g Agar (琼脂)17gDistilled water (蒸储)1000ml pH7.339. Oatemeal Agar (燕麦粉琼月旨)Oatemeal (infusion)燕麦粉浸液2

39、0g Agar (琼脂)18%Trace element solution (迹量盐溶液)1mlDistilled water up to (用蒸馏水补充至1000 毫升) 1000ml pH7.2Note:Trace element solution (迹量盐溶液):FeSO4.7H2O0.1g MnCl2.4H2O0.1gZnSO4.7H2O0.1g Distilled water (蒸馏水) 100ml40. Calcium Malate Agar (苹果酸钙琼脂)Calium malate (苹果酸钙) 10g K2HPO423 / 650.5gNH4Cl0.5g Glycerol (

40、甘油) 10gAgar (琼脂) 15g Distilled water (蒸馏水) 1000ml41. Thiobacillus Ferrooxidans Medium (氧化亚铁硫杆菌培养基)(NH4)2SO40.15g KH2PO40.05gKCl0.05g MgSO4.7H2O0.5gCa(NO3)2.4H2O0.01g FeSO4.7H2O 50gDistilled water (蒸馏水 ) 1000ml pH2.042. Thiobacillus Thiooxidans Medium (氧化硫硫杆菌培养基)(NH4)2SO40.3g KH2PO4 3-4g25 / 65CaCl2

41、(anhydrous) 无水0.25g MgSO4.7H2O0.5gFeSO4.7H2O0.001g Distilled water (蒸馏水) 1000mlpH3.5-443. Plasma Substitute Medium (代血浆培养基)Sucrose (蔗糖)12.5-15% Peptone (蛋白胨)0.5%KH2PO40.03% Na2HPO4.12H2O0.14%pH7.07.244. Skim Milk Medium (脱脂牛奶培养基) ATCC 183脱脂牛奶100g去离子水1000ml适用范围:xx乳杆菌、嗜热乳酸链球菌45. Pea Agar 豌豆( xx)Glucos

42、e (葡萄糖) 1% Peptone (蛋白胨 )0.5%NaCl0.5% Agar (xx)2.0%Pea infusion (counted by NH2)腕豆浸汁(以 NH2 计)12-14 mg / 100pH (before sterilization) 消前 7.27.5Note:Preparetion of pea infusion (豌豆浸汁的制法) :Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to4.34.5 by usingof sulphur

43、ic acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottlemouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37 for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Di

44、stributionandautoclave at 120 for 30 minutes. Store at cool contion . (称取白豌豆900 克,置于5 公升广口瓶中,加自来水3 公升,用硫酸调pH 至4.3-4.5,再加氯仿30毫升(防腐),加胶塞并用纱布扎口(防止冲脱),放37 恒温室 7-10 天。取出用滤纸过滤,滤液煮沸20 分钟,以除氯仿,并再次以滤纸过滤,除沉淀。滤液分装,于120 30 分钟灭菌。后冷藏备用。)The standard quality of pea infusion as following (豌豆浸汁质量标准):Aminonitrogen (氨

45、基酸):160-200 mg / 100mlSoluble phosphoruse (溶磷):400-600 区 g / mlColor (色泽):auratus,transparent (金黄、透明)46. Wheat Bran Medium (麦麸琼脂)Wheat bran (麦麸)3.6% (NH4)2HPO40.3%K2HPO40.02% MgSO4.7H2O0.01%Agar (xx) 2% pH7.0适用范围:金霉素链霉菌、红霉素链霉菌47. Glucose Yeast Extract Agar!(萄糖、酵母膏琼脂)Glucose 翁萄糖)10g Yeast extract (孝母

46、膏)10gWater (水 ) 1000ml Agar (琼脂) 15gpH7.248. MMN Medium (MMN 培养基 )CaCl20.05g MgSO40.15gNaCl0.025g FeCl3 (1%)1.2mlKH2PO40.5g Vitamib B1 硫胺素)100(NH4)2HPO40.25g Wort (12 Be' ) 100mlGlucose (葡萄糖) 10-15g Citric acid 柠檬酸()0.2gDistilled water (蒸馏水) 900ml pH5.5 (左右)适用范围:蜜环菌49. Starch Agar (淀粉琼脂 )Soluble

47、 starch (可溶性淀粉) 10g NaNO3 1gMgCO3 1g K2HPO40.3gNaCl0.5g Agar (xx) 20gDistilled water (蒸馏水) 1000ml50. 5 Be ' Wort Agar (5BI汁琼月旨)适用范围:园弧青霉、少根根霉、xx根霉、xx根霉51. 5 Be ' Koji Extract Agar 傣Be十琼脂)适用范围:华根霉、三宝垄根霉52. 发根农杆菌培养基(YEB)Beef extract (牛肉浸膏) 5g/L Yeast extract (酵母膏 ) 1g/LPeptone (蛋白胨) 5g/L Sucro

48、se 蔗糖() 5g/LMgSO31 / 654.7H2O0.4g/100ml Agar (琼脂)1.5g/100mlpH7.4适用范围:青枯假单胞菌53. 青枯菌培养基(Tm)Peptone (蛋白月东)10g Casein干酪素水解物产)1gGlucose (葡萄糖) 5g Ddhro (纯水 )1000mlAgar(xx) 15g pH7.054. YM (Yeast Malt Agar)酵母膏3.0gxx 汁3.0g蛋白胨5.0gxx20.0g蒸馏水 1000ml55. ISP 4# / 65可溶性淀粉10.0g K2HPO41.0gMgSO4.7H2O1.0g NaCl1.0g(NH

49、4)2SO42.0g CaCO32.0gFeSO4.7H2O0.001g MnCl2.7H2O0.001gxx20.0g 蒸储水 1000ml56. Trypton Soy Agar 胰蛋白胨17.0g大豆月东3.0g葡萄糖2.5gxx20.0gNaCl5.0g K2HPO42.5g蒸馏水 1000ml57. NA (Nutrient Agar)牛肉膏3.0g蛋白月东5.0gxx20.0g 蒸储水 1000mlpH6.67.058. 甘露(糖 )醇 xx(ATCC 1)酵母膏5.0g蛋白月东3.0g甘露(糖 )醇33 / 650.008g15.0g蒸馏水 1000ml59. Marine 琼脂

50、 2216 (ATCC 2)蛋白胨5.0g酵母膏1.0g柠檬酸铁0.1g澳化钾0.08gNaCl19.45g MgCl28.8gNa2SO43.24g CaCl21.8gKCl0.55g NaHCO30.16gNH4NO30.0016g Na2HPO4氯化锶0.034g硼酸0.022g硅酸钠0.004g氟化钠0.0024gxx15.0g 蒸储水 1000mlpH7.47.860. 兔血xx(ATCC 4)牛心(取其浸液)500.0g 胰蛋白月东(Tryptose)10.0gNaCl5.0gxx15.0g蒸馏水 1000ml pH6.6-# / 657.061. 孢子形成培养基(ATCC 5)酵

51、母膏1.0g 牛肉膏1.0g胰蛋白胨2.0g葡萄糖10.0gFeSO4散量 xx15.0g蒸馏水 1000ml pH7.262. Trypticase Soy Agar (ATCC 18)Trypticase Soy Broth30.0gxx15.0g蒸馏水 1000ml63. YPAD (ATCC 109)酵母膏10.0g蛋白月东20.0g37 / 65葡萄糖20.0gxx20.0g蒸馏水 1000ml pH7.064. SP (ATCC 432)棉子糖1.0g蔗糖1.0g半乳糖 1g 可溶性淀粉5g水解酪蛋白2.5gxx20.0gMgSO4.7H2O0.5g K2HPO40.25g蒸馏水

52、1000ml pH 自然65. 胰酪胨大豆酵母浸膏琼脂(TSA-YE)胰蛋白胨15.5g大豆蛋白月东41 / 65NaCl5.0g酵母膏6.5gxx15.0g 蒸储水 1000mlpH7.366. ATCC 20蛋白胨5.0g酵母膏15.0gK2HPO43.0g葡萄糖2.0gxx20.0g 蒸储水 1000mlpH7.37.567. TY (Yeast Tryptone Agar)蛋白胨10.0g酵母膏5.0g葡萄糖1.0g NaCl5.0gxx20.0g 蒸储水 1000mlpH7.068. 硫醇(Thiol)培养基(ATCC 49)示蛋白胨(Proteose Peptone No.3,Di

53、fco)10.0g酵母膏5.0g葡萄糖1.0g NaCl5.0g硫醇复合物8.0gxx1.0gp-氨基苯甲酸2.5g6.97.369. CzapeK Dox 琼脂培养基(ATCC 134)Czapek Dox Broth35.0gxx15.0g蒸馏水 1000ml70. MG/L蛋白胨5.0gxx5.0g谷氨酸钠1.15g 生物素0.0001gK2HPO40.25g NaCl0.1gMgSO4.7H2Oxx20.0g 蒸储水 1000ml氯霉素(终浓度)50止g/mlpH7.071. Eclb Broth蛋白胨10.0g酵母膏5.0gNaCl5.0gxx20.0g蒸储水1000ml氨节青霉素(终浓度)300 wg/mlpH7.0酵母膏1.0g 牛肉膏1.0gNZ Amine,type A2.0g葡萄糖43 / 6517.5gxx20.0g 蒸储水 1000mlpH7.373. Harrold /

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