医学教材 丙型肝炎病毒(基础)

丙型肝炎病毒邢文革

2014-12-17石家庄丙型肝炎病毒的发现1970’s:HepatitisAandBdiagnosticsMosttransfusionassociatedhepatitisnotcausedbyHAVandHBVorotherknownviralagents(NEJM292:767;Gastro.69:1278;JID139:511;VoxSang.44:231)non-A,non-Bhepatitis(NANBH)Mightbeasmall,envelopedvirustransmissibletochimpanzees(Gastro88:773;JID156:636)StandardimmunologicalmethodsfailedtoidentifyviralantibodiesorantigensChirongroupThoughtthattherewasnotenoughviralantigeninNANBHSotheydecidedtotryandconcentratethevirus丙型肝炎病毒的发现1.传染性肝炎(HA)血清型肝炎(HB)2.动物模型（黑猩猩）3.基因文库4.放射免疫标记5.全基因测序HightiterNANBHchimpanzeeplasmaUltracentrifugationat104,000gfor5hoursExtractnucleiacid,randomprimedcDNAintogt11phageImmunoscreenedwithNANBHpatientserumClone5-1-1LargeroverlappingClone81丙型肝炎病毒的发现PhagewithportionsofHCVgenomeInfectE.coliPhagekillsE.coliandleavesplaquesScreenwithHCVPatientserumLibraryScreening丙型肝炎病毒的发现WesternblotClone5-1-1expressedasSODfusionproteincalledPS5PS5SODNANBHpatientserumExperimentallyinfectedchimpserumProteinfromclone5-1-1closelyassociatedwithNANBHinfectionsHCVwasthefirstvirustobeisolatedandcharacterizedsolelybymolecularmethods,iewithoutculturemethods黑猩猩易感HCV病原学黄病毒科（Flaviviridae）丙型肝炎病毒属包膜糖蛋白包膜+ssRNA核衣壳HepatitisCGenome

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