动物蛋白替代花鲈饲料中的鱼粉

Effectsoffishmealqualityandfishmealsubstitutionbyanimalproteinblendongrowthperformance，GH/IGF-IaxisandfleshqualityofJapaneseseabass(Lateolabraxjaponicus)

HU.L.

,XUEM*.

WANGL,WUX,GEH,ZHENGY

NationalAqua-feedSafetyAssessmentStation,FeedResearchInstitute,CAASISFNF2010,Qingdao,CHINA动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第1页!NationalAqua-feedSafetyAssessmentStation,FRI,CAAS,Beijing,P.R.CHINA动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第2页!Japaneseseabass(Lateolabraxjaponicus)CarnivorousWidelyculturedinbothsaltyandfreshwaterHighestproductionamongmarinespeciesinChina.动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第3页!4BackgroundandJustificationFishmealsubstitution:Supply,price,sustainabilityAnimalproteinblend:Aminoacidplementation,costsavings,sustainability,PalatabilityforCarnivorousfishspecies(vs.plantsources)DIAA:MoreprecisethandeterminedAAlevel,lowercrudeproteinformulation,reducingwaste,动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第4页!Materials&methodsIBW:

76.2±0.08gPositiveContorl:PeruFM（PFM）NegativeContorl:

LocalFM（LFM）withhighVBN(148mg/kg)Alternativeproteinresources:

Animalproteinblend (APB) PBM:MBM:BM:HFeM=40:35:20:5

Substitutionlevel:20%、40%、60%、80%ofPFM动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第5页!Formulationandproximatepositionofexperimentaldiets

(g.kg-1,wetbasis)

ItemsPFMLFMAPB20APB40APB60APB80

Proximateanalysis(wetbasis)Moisture(%)57.2555.4681.0486.0379.5979.75Ash(%)101.44119.2698.7697.2696.1497.10Crudeprotein(%)443.40449.80434.70434.40434.30436.00Grossenergy(MJ·kg-1)195.20197.10190.60190.80189.70190.10Crudelipid(%)104.80102.2098.00101.40Idealproteinconceptbasedondigestibleaminoacidsprofile(wetbasis)Digestibleprotein(g•kg-1)383.2383.3384.7386.0383.4385.3Digestibleenergy(MJ•kg-1)15.1715.4415.1715.2315.0415.18D-Lys/DP(%)7.197.007.177.147.197.16D-Met/DP(%)2.432.142.422.412.432.42D-Thr/DP(%)3.673.793.663.653.673.65DP/DE(%)25.2624.8325.3625.3525.4925.39动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第6页!Materials&methodsFeed&Feeding:twomealsperdaytosatiationwithextrudeddietsGrowthperiod:25fishpertankwithtriplicatesRearingsystem:recirculation,25-28CDuration:8w动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第7页!Weightgainrate(WGR)ofJSBduringtheexperiment动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第8页!Hepato-somaticindex(HSI)&perivisceralfatindex(PFI)

ofJSBduringtheexperiment动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第9页!Figure2SamplingdistributionABCA:usedfordriplossB:usedfortextureprofileanalysisC:usedforNIR&sensoryassessmentD:verticallinesD动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第10页!Figure3Texturecurveandcalculationsfortextureparameters

RafaelGines,etal.FoodQualityandPreference15(2004)177–185.动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第11页!EffectsofdifferentlevelofAPBonthetexturalcharactersofcookedJSB’sfillets动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第12页!Effectsofexperimentaldietsontextureprofilesofcookedseabass动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第13页!动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第14页!Visualpathologicalchangesrelatedwithdiets

BesidesgroupPFM,fishfedtheother5dietsdisplayedsymptomsofskin-inflammationinthemiddleoftheexperiment,however,fishingroupLFM&APB20wereself-curedintheendofthetrial.Survivalofallgroupsarehigherthan97%.动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第15页!ConclusionNosignificantnegativeeffectongrowthperformancewasdetectedwhendietaryfishmeallevelwasreducedfrom40%to24%and40%werereplacedbyAPBunderDIAA.FishmealwithhighVBNshowedsignificantnegativeeffectondietpalatability,higherinclusionlevelneedtobeusedinformulationasDIAAprofile:ReducingPFMfrom40%to8%byaddinganimalproteinblendwouldaffectthetexturalcharactersofJSB’sfilletsandtaste.动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第16页!Literaturesreview

Manyfactorssuchasculturingsystem,husbandry,fishspecieswouldaffectthetrialconclusions,besides,thequalityandquantityoftheFMusedinthecontrolarethemostimportant!动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第17页!Formulationandproximatepositionofexperimentaldiets

(g.kg-1,wetbasis)

IngredientsPFMLFMAPB20APB40APB60APB80Perufishmeal400.000.00320.00240.00160.0080.00Localfishmeal0.00500.00

0.000.000.000.00Animalproteinblend0.000.0080.00160.00240.00320.00Soybeanmeal210.00110.00210.00210.00200.00200.00Wheatflour220.00220.00220.00220.00220.00220.00Fishoil50.0042.0046.3043.6049.3040.60L-lysine(50%)0.000.002.464.937.9110.34DL-methionine(98%)0.000.000.721.452.242.94L-threonine(98%)0.000.000.490.971.602.07Others120.00128.00120.00119.00119.00124.00动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第18页!Essentialaminoacidprofileofthetestdiets

(g.kg-1,wetbasis)

ItemsPFMLFMAPB20APB40APB60APB80threonine17.0015.9016.7016.3016.7017.00valine19.1018.1019.4019.3018.9021.30methionine10.209.409.909.809.409.80isoleucine17.6016.8016.3015.2014.4014.80leucine31.5030.1031.2031.4032.6033.50phenylalanine18.6017.1018.1018.1018.5019.00lysine29.2027.6030.2029.8030.7031.00histidine10.808.7012.3010.4012.2012.40arginine25.6023.2026.0025.8026.3025.80SumofEAA179.60166.90180.10176.10179.70184.60TotalofAA385.60363.00390.50382.50391.20399.30EAA/TAA0.470.460.460.460.460.46动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第19页!Feedintake(FI)ofJSBduringtheexperiment动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第20页!Feedconversionratio(FCR),proteinefficiencyratio(PER)

ofJSBduringtheexperiment动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第21页!Figure1SamplingsitesforpHJ.SUONTAMA,etal.AquacultureNutrition200612;1–9.动物蛋白替代花鲈饲料中的鱼粉共29页，您现在浏览的是第22页!EffectsofdifferentlevelofAPBondroploss