2-Hydroxypropyl-β-cyclodextrin-Hydroxypropyl-betadex-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemE(2-Hydroxypropyl)-β-cyclodextrinCat.No.:HY-101103CASNo.:128446-35-5Synonyms:Hydroxypropylbetadex;Hydroxypropyl-β-cyclodextrin;HP-β-CD作⽤靶点:Others作⽤通路:Others储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:50mg/mL(Needultrasonic)H2O:50mg/mL(Needultrasonic)请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(InfinitymM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(InfinitymM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(InfinitymM);Clearsolution4.请依序添加每种溶剂：PBSSolubility:100mg/mL(InfinitymM);Clearsolution;Needultrasonicandwarmingandheatto60°C5.请依序添加每种溶剂：SalineSolubility:200mg/mL(InfinitymM);Clearsolution;Needultrasonic1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEBIOLOGICALACTIVITY⽣物活性(2-Hydroxypropyl)-β-cyclodextrin⼴泛使⽤的药物递送载体，可提⾼稳定性和利⽤度。体外研究Celltreatmentwith(2-Hydroxypropyl)-β-cyclodextrinresultsintheactivationofthetranscriptionfactorEB,amasterregulatoroflysosomalfunctionandautophagy,andinenhancementofthecellularautophagicclearancecapacity[1].(2-Hydroxypropyl)-β-cyclodextrintreatmentreducesintracellularcholesterolresultinginsignificantleukemiccellgrowthinhibitionthroughG2/Mcell-cyclearrestandapoptosis.TheIC50valuesfor(2-Hydroxypropyl)-β-cyclodextrinafter72hoursexposureareintherangeof3.86–10.09mM.(2-Hydroxypropyl)-β-cyclodextrinalsoshowsanticancereffectsagainstCMLcellsexpressingaT315IBCR-ABLmutation(thatconfersresistancetomostABLtyrosinekinaseinhibitors),andhypoxia-adaptedCMLcellsthathavecharacteristicsofleukemicstemcells.Inaddition,colonyformingabilityofhumanprimaryAMLandCMLcellsisinhibitedby(2-Hydroxypropyl)-β-cyclodextrin[2].体内研究(2-Hydroxypropyl)-β-cyclodextrinadministrationpromotestranscriptionfactorEB-mediatedclearanceofproteolipidaggregatesthataccumulateduetoinefficientactivityofthelysosome-autophagysystemincellsderivedfromapatientwithalysosomalstoragedisorder[1].Intraperitonealinjectionof(2-Hydroxypropyl)-β-cyclodextrinsignificantlyimprovessurvivalinleukemiamousemodels.Systemicadministrationof(2-Hydroxypropyl)-β-cyclodextrintomicehasnosignificantadverseeffects[2].PROTOCOLCellAssay[2]Cellsareincubatedwith(2-Hydroxypropyl)-β-cyclodextrinatvariousconcentrations(5,7.5,10,15,20mM)for72hours.Cellviabilityisassessedusingatrypanbluedyeexclusionmethodandcellproliferationisevaluatedusingamodifiedmethyl-thiazol-diphenyl-tetrazolium(MTT)assay[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.AnimalMice:Miceareintraperitoneallyinjectedwith200μLvehicle(saline)or(2-Hydroxypropyl)-β-cyclodextrin(50Administration[2]or150mM)for20consecutivedays3daysaftertransplantation,andsurvivalismonitoreddaily.LeukemiccellengraftmentisconfirmedbydetectionofGFP-positivecellsintherecipient’sBMusingflowcytometry[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•NatCommun.2022May31;13(1):3022.•CellRep.2018Dec11;25(11):2972-2980.e5.•CancerCellInt.2021Apr15;21(1):216.•FrontCellInfectMicrobiol.2021Jul23;11:705087.•IntImmunopharmacol.2021Apr17;96:107643.Seemorecustomervalidationsonwww.MedChemEREFERENCES2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[1].SongW,etal.2-Hydroxypropyl-β-cyclodextrinpromotestranscriptionfactorEB-mediatedactivationofautophagy:implicationsfortherapy.JBiolChem.2014Apr4;289(14):10211-22.[2].YokooM,etal.2-Hydroxypropyl-β-CyclodextrinActsasaNovelAnticancerAgent.PLoSOne.2015Nov4;10(11):e01