微流体芯片课件

MultipleandHigh-ThroughputDropletReactionsviaCombinationofMicrosamplingTechniqueandMicrofluidicChipMembers:李娜冯双霞徐秋燕刘婷婷聂怀军themegalleryMultipleandHigh-ThroughputD1ContentsBackgroundExperimentABSTRACTDiscussionContentsBackgroundExperimentA2ABSTRACTMicrodropletsofferuniquecompartmentsforaccommodatingalargenumberofchemicalandbiologicalreactionsintinyvolumewithprecisecontrol.Amajorconcernindroplet-basedmicrofluidicsisthedifficultytoaddressdropletsindividuallyandachievehighthroughputatthesametime.ABSTRACTMicrodropletsofferuni3Here,wehavecombinedanimprovedcartridgesamplingtechniquewithamicrofluidicchiptoperformdropletscreeningsandaggressivereactionwithreagentconsumption.Ourcombineddeviceprovidesasimplemodeltoutilizemultipledropletsforvariousreactionswithlowreagentconsumptionandhighthroughput.Here,wehavecombinedanimpr4微流体芯片课件5BackgroundAlargenumberofreactionscanbeindependentlyminiaturizedintotinydropletsrangingfromnanolitertopicolitersizeswithouthavingtoincreasethechipsizeandcomplexity.

Microfluidicchipshaveprovidedapowerfulplatformforaccomplishingdropletformationandmanipulationwithinonechipofonlyafewsquarecentimetersinsize.

AdvantagesOverthepastfewyears,droplet-basedmicrofluidicshasattractedincreasingattention.BackgroundAlargenumberofre6Background

Dropletgenerationformultiplesamplesislimitedbythe“world-to-chip”interfaceproblemandcannotbeaddressedindividuallyDropletgenerationandmanipulationfunctionswithinonechipcaneasilyinterferewitheachotherandaredifficultTobalance,despitebeingseparatedbyalong,windingchannel.DrawbackPassivedropletformationmethods:BackgroundDropletgeneratio7InnovateThiscontextshowsahybriddevice:thecartridgedropletgenerationtechniquecombinedwithamicrofluidicchip.Theyimprovedthecartridgetechniquewithanovelmovementscheme:thecapillary,insteadofthemultiwellplate,wasmovedbyadigitalandautomaticmanipulatortoaspiratedifferentsamplesorthecoveringoil.InnovateThiscontextshowsah8Thehybriddropletdeviceforgenerationandmanipulationconsistsoffourmajorcomponents.

(a)manipulator(b)multiwellplate(c)capillary(d)PDMSchipThehybriddropletdevicefor9微流体芯片课件10微流体芯片课件11Threegeneralsteps

loadthemultiwellplatewithdifferentsamples,coverwithalayerofoil,andinputtheparametersofthedesireddropletarrayintothecontrollerviaaPC

startthecontrollertoaspiratecertainvolumesofsamplesandoilintotheCapillarysequentially

introducethegenerateddropletsintothePDMSchipformerging,mixing,detection,orothermanipulation.Threegeneralstepsloadthem12Laboratoryreagent

siliconeoil,

FeCl2·4H2O(Sigma−Aldrich),FeCl3·6H2O(AcrosOrganics),ammoniumhydroxidesolution(28%NH3Fluka),andhydrochloricacid(37%HCl,AcrosOrganics)Laboratoryreagentsiliconeo13ImprovedSamplingTechnique

CombinedDropletDevicePerformance

High-ThroughputDropletScreening

AggressiveDropletReaction

ImprovedSamplingTechniqueCo14ImprovedSamplingTechniqueThecapillaryaspiratestheoilwhenthecantileverremainsinthe“up”state.Whenthecantileveristriggeredtostayinthe“down”state,thecapillaryaspiratesthesample.Theresidencetimeintheoil(TO)andsample(TS)couldbeadjustedrespectivelytogeneratedropletswithdesireddistanceandvolume.ImprovedSamplingTechniqueThe15微流体芯片课件16(c)Relativeintensitywasmeasuredalongthechannelstartingfromtoprightcorner.TheintensityoffluoresceinandDIwaterdropletdoesnotdecreaseorincrease,whichindicatesthatnocross-contaminationbetweenthesetwosampleswasdetected.(b)Dropletarraywasgeneratedbysequentiallyaspirating50uMfluoresceinandDIwater.(c)Relativeintensitywas17CombinedDropletDevicePerformanceHere,themergingofdropletswasdemonstratedinaPDMSchip.ThedropletsfusiondoesnotdependondropletdistancebutdropletvelocityandVd/Vc.Aftermixingcompletely,themergeddropletwasoutputtoasinuouschannelfordisplayanddetection.CombinedDropletDevicePerfor18微流体芯片课件19Inthefirstapplication,aseriesofAiandBm,n(i=1−2,m=1−3,andn=1−6)weregenerated.A1andA2areFluo-4andRhod-2solutionswiththesameconcentration(10.8μM),whichareindicatorsthatexhibitanincreaseinfluorescence(green/red)uponmetalionbonding.Avalueofm=1−3representmetalsionsolutionsofLaIII,YbIII,andYIII,respectively,whilen=1−6aresixdifferentmetalionconcentrations(0,2.5,5,10,20,and40μM)ofthesesolutions.Thedropletarraycontains721-nLdropletsInthefirstapplication,aser20High-ThroughputDropletScreeningHigh-ThroughputDropletScreen21微流体芯片课件22The36pairsofdropletreactionsused∼72nLofsolutionandrequired1.8mintocomplete.Therefore,thereagentconsumptionforeachmeasurementcouldbereducedto1nLpersample,andthereactionthroughputis1200h−1,whichcanbemuchfasterbyincreasingtheflowvelocity.ThescreeningdropletscouldbestoredinthePDMSchipafterwestoppedthevacuum.The36pairsofdropletreacti23AggressiveDropletReactionAggressiveDropletReaction24Fromtheseimages,wefoundthatthecoprecipitationissofastthatitimmediatelyformsparticleswhentwodropletscomeintocontactwitheachother.Moreover,ineachvolumeratio,theprecipitationinitiatedbythefusionoftwodropletsdidnotblockthemicrochannelatsuchhighconcentrations.Suchflexible,controllableandminiaturizedreactionconditionsareextremelysuitableforaggressive,fast,orevenexplosivereactionsthatgenerateprecipitationorheat.Fromtheseimages,wefoundt25DiscussionThecartridgedropletgenerationmethodprovidesanefficientwayforintroducingdifferentnanoliterdropletsintothemicrofluidicchip.themicrofluidicchipoffersaplatformfordelicatemanipulationsuchasmerging.Thisfeatureisvaluableforreducingthereactionscreeningandoptimizationcostandtimeandisparticularlyusefulfordrugscreening.DiscussionThecartridgedrople26Comparedwiththeothercartridgedropletgeneration,ourdesignusedadigitalandautomaticmanipulatortomovethecapillaryinsteadofthemultiwellplate.DiscussionSubsequently,thecoveringoilwasingeniouslyusedasacarrierfluid(continuous-phase)toavoidevaporationandcross-contamination.Comparedwiththeothercartri27Thedropletgenerationthroughputsinoursamplingdeviceincreasedto1−5sperdroplet,whichisefficientandversatileforarbitrary,multiple,andhigh-throughputdropletgenerationandcouldbeincreasedbyincreasingtheflowvelocity.Thedropletgenerationthrough28Thisimprovedcartridgetechniqueandmicrofluidicchipwereintegratedtomakeuseoftheirrespectiveadvantagesforperformingresponsecalibrationsandaggressivereactionandscreeningmultiplereactionconditionswithminimal(nL)reagentconsumption.Thisimprovedcartridgetechni29ThankYou!2019.11.19themegalleryThankYou!2019.11.19themegall30docin/sanshengshiyuandoc88/sanshenglu

更多精品资源请访问docin/sanshengshiyuan更多精品31MultipleandHigh-ThroughputDropletReactionsviaCombinationofMicrosamplingTechniqueandMicrofluidicChipMembers:李娜冯双霞徐秋燕刘婷婷聂怀军themegalleryMultipleandHigh-ThroughputD32ContentsBackgroundExperimentABSTRACTDiscussionContentsBackgroundExperimentA33ABSTRACTMicrodropletsofferuniquecompartmentsforaccommodatingalargenumberofchemicalandbiologicalreactionsintinyvolumewithprecisecontrol.Amajorconcernindroplet-basedmicrofluidicsisthedifficultytoaddressdropletsindividuallyandachievehighthroughputatthesametime.ABSTRACTMicrodropletsofferuni34Here,wehavecombinedanimprovedcartridgesamplingtechniquewithamicrofluidicchiptoperformdropletscreeningsandaggressivereactionwithreagentconsumption.Ourcombineddeviceprovidesasimplemodeltoutilizemultipledropletsforvariousreactionswithlowreagentconsumptionandhighthroughput.Here,wehavecombinedanimpr35微流体芯片课件36BackgroundAlargenumberofreactionscanbeindependentlyminiaturizedintotinydropletsrangingfromnanolitertopicolitersizeswithouthavingtoincreasethechipsizeandcomplexity.

Microfluidicchipshaveprovidedapowerfulplatformforaccomplishingdropletformationandmanipulationwithinonechipofonlyafewsquarecentimetersinsize.

AdvantagesOverthepastfewyears,droplet-basedmicrofluidicshasattractedincreasingattention.BackgroundAlargenumberofre37Background

Dropletgenerationformultiplesamplesislimitedbythe“world-to-chip”interfaceproblemandcannotbeaddressedindividuallyDropletgenerationandmanipulationfunctionswithinonechipcaneasilyinterferewitheachotherandaredifficultTobalance,despitebeingseparatedbyalong,windingchannel.DrawbackPassivedropletformationmethods:BackgroundDropletgeneratio38InnovateThiscontextshowsahybriddevice:thecartridgedropletgenerationtechniquecombinedwithamicrofluidicchip.Theyimprovedthecartridgetechniquewithanovelmovementscheme:thecapillary,insteadofthemultiwellplate,wasmovedbyadigitalandautomaticmanipulatortoaspiratedifferentsamplesorthecoveringoil.InnovateThiscontextshowsah39Thehybriddropletdeviceforgenerationandmanipulationconsistsoffourmajorcomponents.

(a)manipulator(b)multiwellplate(c)capillary(d)PDMSchipThehybriddropletdevicefor40微流体芯片课件41微流体芯片课件42Threegeneralsteps

loadthemultiwellplatewithdifferentsamples,coverwithalayerofoil,andinputtheparametersofthedesireddropletarrayintothecontrollerviaaPC

startthecontrollertoaspiratecertainvolumesofsamplesandoilintotheCapillarysequentially

introducethegenerateddropletsintothePDMSchipformerging,mixing,detection,orothermanipulation.Threegeneralstepsloadthem43Laboratoryreagent

siliconeoil,

FeCl2·4H2O(Sigma−Aldrich),FeCl3·6H2O(AcrosOrganics),ammoniumhydroxidesolution(28%NH3Fluka),andhydrochloricacid(37%HCl,AcrosOrganics)Laboratoryreagentsiliconeo44ImprovedSamplingTechnique

CombinedDropletDevicePerformance

High-ThroughputDropletScreening

AggressiveDropletReaction

ImprovedSamplingTechniqueCo45ImprovedSamplingTechniqueThecapillaryaspiratestheoilwhenthecantileverremainsinthe“up”state.Whenthecantileveristriggeredtostayinthe“down”state,thecapillaryaspiratesthesample.Theresidencetimeintheoil(TO)andsample(TS)couldbeadjustedrespectivelytogeneratedropletswithdesireddistanceandvolume.ImprovedSamplingTechniqueThe46微流体芯片课件47(c)Relativeintensitywasmeasuredalongthechannelstartingfromtoprightcorner.TheintensityoffluoresceinandDIwaterdropletdoesnotdecreaseorincrease,whichindicatesthatnocross-contaminationbetweenthesetwosampleswasdetected.(b)Dropletarraywasgeneratedbysequentiallyaspirating50uMfluoresceinandDIwater.(c)Relativeintensitywas48CombinedDropletDevicePerformanceHere,themergingofdropletswasdemonstratedinaPDMSchip.ThedropletsfusiondoesnotdependondropletdistancebutdropletvelocityandVd/Vc.Aftermixingcompletely,themergeddropletwasoutputtoasinuouschannelfordisplayanddetection.CombinedDropletDevicePerfor49微流体芯片课件50Inthefirstapplication,aseriesofAiandBm,n(i=1−2,m=1−3,andn=1−6)weregenerated.A1andA2areFluo-4andRhod-2solutionswiththesameconcentration(10.8μM),whichareindicatorsthatexhibitanincreaseinfluorescence(green/red)uponmetalionbonding.Avalueofm=1−3representmetalsionsolutionsofLaIII,YbIII,andYIII,respectively,whilen=1−6aresixdifferentmetalionconcentrations(0,2.5,5,10,20,and40μM)ofthesesolutions.Thedropletarraycontains721-nLdropletsInthefirstapplication,aser51High-ThroughputDropletScreeningHigh-ThroughputDropletScreen52微流体芯片课件53The36pairsofdropletreactionsused∼72nLofsolutionandrequired1.8mintocomplete.Therefore,thereagentconsumptionforeachmeasurementcouldbereducedto1nLpersample,andthereactionthroughputis1200h−1,whichcanbemuchfasterbyincreasingtheflowvelocity.ThescreeningdropletscouldbestoredinthePDMSchipafterwestoppedthevacuum.The36pairsofdropletreacti54AggressiveDropletReactionAggressiveDropletReaction55Fromtheseimages,wefoundthatthecoprecipitationissofastthatitimmediatelyformsparticleswhentwodropletscomeintocontactwitheachother.Moreover,ineachvolumeratio,theprecipitationinitiatedbythefusionoftwodropletsdidnotblockthemicrochannelatsuchhighconcentrations.Suchflexible,controllableandminiaturizedreactionconditionsareextremelysuitableforaggressive,fast,orevenexplosivereactionsthatgenerateprecipitationorhea