TMRE-Tetramethylrhodamine-ethyl-ester-perchlorate-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemETMRECat. No.: HY-D0985ACAS No.: 115532-52-0Synonyms: Tetramethylrhodamine ethyl ester perchlorate分式: CHClNO分量: 514.95作靶点: Others作通路: Others储存式: 4C, protect from light* In solvent : -80C, 6 months; -20C, 1 month (protect fromligh

2、t)溶解性数据体外实验 DMSO : 150 mg/mL (291.29 mM)* means soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 1.9419 mL 9.7097 mL 19.4194 mL5 mM 0.3884 mL 1.9419 mL 3.8839 mL10 mM 0.1942 mL 0.9710 mL 1.9419 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 TMR

3、E种线粒体特异性染料 (ex=550 nm，em=575 nm)。体外研究TMRE is a mitochondria specific dye (ex=550 nm, em=575 nm) 1. Multidirectional dynamic movement ofTMRE is observed in epithelial cells and bidirectional dynamic movement is seen in the superficial corticalfiber cells of live bovine lenses. In the epithelium, the

4、movement of TMRE fluorescence is up to 5 m/minwhereas in the superficial cortex the observed movement is up to 18.5 m/min. The movement of TMRE1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEfluorescence is abolished with treatment of the uncoupler, carbonyl cyanide m-chlorophenylhydrazone(CCCP) 2.

5、PROTOCOLCell Assay 1 The entire experiment should be performed at room temperature because temperature will directly impactmitochondrial transmembrane potential and TMRE staining. Cells should never be placed, centrifuged,incubated, or washed at 4C or have ice-cold buffers or media added. Treat the

6、cells with a cytotoxicstimulus. Harvest cells and resuspend at 5105 cells/mL in culture medium containing 150 nM TMRE.Incubate for 5 min at room temperature in the dark. Add Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) (5 M final concentration) to an aliquot of untreated cells andincu

7、bate for 5 min at room temperature in the dark. Turn on the appropriate laser on the flow cytometer. Setup a histogram plot to detect TMRE using log scale 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Crowley LC, et al. Measuring Mitoch

8、ondrial Transmembrane Potential by TMRE Staining. Cold Spring Harb Protoc. 2016 Dec1;2016(12):t087361.2. Bantseev V, et al. Confocal laser scanning microscopy imaging of dynamic TMRE movement in the mitochondria of epithelial andsuperficial cortical fiber cells of bovine lenses. Mol Vis. 2005 Jul 14;11:518-23.McePdfHeightCaution: Product has not been fully validated for medical application