常用酿酒酵母菌株基因型

1、Com mon ly used stra insThis page describes some of the most commonly used yeast lab strains. Much of the information is taken from | F.Sherman (2002) Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include:Mortimer and Johnston (1986) Ge

2、netics 113:35-43 - thoroughly describes the genealogy of strain S288Cvan Dijken et al. (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly | used lab strains |Winzeler et al. (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study gene

3、tic identity between common lab strainsS288CGenotype: MAT aSUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form | pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain

4、 for mitochondrial studies. It has an allelic variant of MIP1 which increases petite frequency. S288C strains are|gal 2- and they do not use galactose |anaerobically.The S288C genome was recently resequenced at the | Sanger Institute .References: | Mortimer and Johnston (1986) Genetics 113:35-43. |S

5、ources: |ATCC:204508BY4743Genotype: |MAT a/ ahis3 1/his3 A1 leu2 M/leu2 山 LYS2/lys2 山 met15 M/MET15 ura3 M/ura3 山Notes: Strain used in thesystematic deletion project |, generated from a cross between BY4741 and BY4742, which are derived from S288C. As S288c, these strains have an allelic variant of

6、MIP1 which increases petite frequency. | See Brachmann et al. reference for details. |References: | Brachmann et al. (1998) Yeast 14:115-32. |Sources: | Biosystems:YSC1050FY4Genotype: | MAT aNotes: Derived from S288C. |References: |Winston et al. (1995) Yeast 11:53-55. |Brachmann et al. (1998) Yeast

7、 14:115-32. |FY1679Genotype: MAT a/ aura3-52/ura3-52 trpl A63/TRP1 Ieu2 A1/LEU2 his3 A200/HIS3 GAL2/GALNotes: Isogenic to S288C; usedin the systematic sequencing project, the sequence stored in SGD.References: |Winston et al. (1995) Yeast 11:53-55.Sources: EUROSCARF:10000DAB972Genotype:- MAT aX2180-

8、1B trp1 0 rho 0Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B- trp1.References: | Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830. |Sources: ATCC:204511A364AGe

9、notype: MAT a ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIONotes: Used in the systematic sequencing project, the sequence stored in SGD.References: | Hartwell (1967) J. Bacteriol. 93:1662-1670. |Sources: |ATCC:208526XJ24-24aGenotype: MAT a ho HMa HMt ade6 arg4 -17 trp1-1 tyr7-1 MAL2Notes: Deriv

10、ed from, but not isogenic to, S288C|References: | Strathern et al. (1979) Cell 18:309-319 |DC5Genotype:MAT a leu2-3,112 his3-11,15 can1-11Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References: | Broach et al. (1979) Gene 8:121-133 |X2180-1AGenotyp

11、e: MAT a SUC2 mal mel gal2 CUP1Notes: S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b wereobtained from sporulated X2180 |References: Mortimer and JohnstonSources: |ATCC:204504YN N216Genotype: MAT a/ aura3-52/ura3-52 Iys2-8O1 amber/Iys2-8O1 amber a

12、de2-101 ochre/ade2-101 ochreNotes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin). |References: | Sikorski RS and Hieter P (1989) Genetics 122:19-27. |Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.YPH499Genotype: MAT a ura3-52 lys2-801_ambe

13、r ade2-101_ochre trp1-出3 his3- A200 leu2- A1Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note|thattrp1-出3, unlike trp1- A1, does not delete adjacent |GAL3 UAS sequence and retains homology to | TRP1 selectable marker. gal2-, does not use g

14、alactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis | 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.|References: | Sikorski RS and Hieter P (1989) Genetics 122:19-27. |Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.Johnston

15、M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.Sources: |ATCC:204679YPH500Genotype: MAT aura3-52 lys2-801_amber ade2-101_ochre trp1-出3 his3- A200 leu2- A1Notes: MAT a strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216(Johnston and Davis 1984; original sour

16、ce: M. Carlson, Columbia University), which is congenic with S288C.References: | Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.Sources: |ATCC:204680YPH501Genotype: MAT a/MAT a ura3-52/ur

17、a3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochretrp1- 63/trp1-出3 his3- 200/his3- A200 leu2- A1/leu2- A1Notes: |a/ a diploid isogenic to YPH499 and YPH500 . Derived from the diploid strain YNN216 (Johnston and Davis1984; original source: M. Carlson, Columbia University), which is con

18、genic with S288C.|References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.Sources: |ATCC:204681Sigma 1278BNotes: Used in pseudohyphal growth studies. Detailed notes about the sigma str

19、ains have been kindly provided by Cora Styles. |Granek and Magwene , PLoS Genet. 2010 Jan 22;6(1):e1000823, established that certain lineages of the |Sigma1278B background contain a nonsense mutation in RIM15, a G-to-T transversion at position 1216 that converts a Gly codon to an opal stop codon. Th

20、is rim15 mutation interacts epistatically with mutations in certain other genes to affect colony morphology. |Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found | here.SK1Genotype: | MAT a/ aHO gal2 cup S can1 R BIONotes: Commonly used for study

21、ing sporulation or meiosis. Canavanine-resistant derivative.The SK1 genome was sequenced at the | Sanger Institute |.References: | Kane SM and Roth J. (1974) Bacteriol. 118: 8-14 |Sources: ATCC:204722CEN.PK (aka CEN.PK2)Genotype: MAT a/ aura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his31/

22、his3 A1 MAL2-8C/MAL2-8CSUC2/SUC2Notes: CEN.PK possesses a mutation in CYR1 (A5627T corresponding to a K1876M substitution near the end of the catalytic domain in adenylate cyclase which eliminates glucose- and acidification-induced cAMP signalling and delays glucose-induced loss of stress resistance

23、 ( Vanhalewyn et al., 1999 |; Dumortier et al., 2000 |).References: | van Dijken et al. (2000) Enzyme Microb Technol 26:706-714 Sources: EUR0SCARF:30000DW303Genotype: MATa/MAT a eu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 ph门Notes: W303also contains abud4 mutation that causes haploids to bud

24、with a mixture of axial and bipolar buddingpatterns. In addition, the original W303 strain contains the| |rad5-535allele. As S288c, W303 has an allelic variantofMIP1 which increases petite frequency.The W303 genome was sequenced at the Sanger Institute .References:W303 constructed by Rodney Rothstei

25、n (see detailed notes from RR and Stephan Bartsch ).bud4 info: Voth et al. (2005) Eukaryotic Cell, 4:1018-28. rad5-535 info: Fan et al. (1996) Genetics 142:749|Sources: |Bio；W303-1AGenotype: MAT a leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15Notes: W303-1A possesses a |ybp1-1 mutation (I7L, F3

26、28V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.References: W303 constructed by Rodney Rothstein ( see detailed notes from RR and Stephan Bartsch ).ybp1-1 info: Veal et al. (2003) J. Biol. Chem. 278:30896-904.Sources: | Biosystems:YSC1058W303-1BGenotype:

27、 MAT a 9u2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15References: W303 constructed by Rodney Rothstein ( see detailed notes from RR and Stephan Bartsch ).Sources: | Biosystems:YSC1058W303-K6001Genotype: MAT a; ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, GAL, psi+, ho:HO:CDC6 (at HO), cdc6:h

28、isG, ura3:URA3 GAL-ubiR-CDC6 (at URA3) References: K6001 was developed by | Bobola et al in Kim Nasmyth's lab (PMID: 8625408), and has become a common model in yeast aging research (PMID: 15489200). Its genome has been sequenced by Timmermann et al (PMID: 20729566)D273-10BGenotype: MAT amalNotes

29、: Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression. |References: Sherman,

30、 F. (1963) Genetics 48:375-385.Sources: |ATCC:24657FL100Genotype: | MAT aReferences: | Lacroute, F. (1968) J. Bacteriol. 95:824-832. |Sources: ATCC: 28383 |SEY 6210/SE Y6211Genotype: |MAT a/MAT a leu2-3,112/leu2-3,112 ura3-52/ura3-52 his3-A200/his3- A200 trp1- A901/trp1- A9011ade2/ADE2 suc2- A9/suc2

31、-血 GAL/GAL LYS2/lys2-801Notes: SEY6210/SEY6211, also known as SEY6210.5, was constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman)

32、. It has several selectable markers, good growth properties and good sporulation.References: | Robinson et al. (1988) Mol Cell Biol 8(11):4936-48 |Sources: |ATCC:201392SEY6210Genotype: MAT aleu2-3,112 ura3-52 his3- A200 trp1- A901 suc2-出 lys2-801; GALNotes: SEY6210 is a MATalpha haploid constructed

33、by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.References: | Robinson et

34、al. (1988) Mol Cell Biol 8(11):4936-48 |Sources: |ATCC:96099SEY6211Genotype: |MAT a leu2-3,112 ura3-52 his3- A200 trp1- A901 ade2-101 suc2- A9; GALNotes: SEY6211 is a MATa haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing w

35、ith strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties. |References: | Robinson et al. (1988) Mol Cell Biol 8(11):4936-48 |Sources: |ATCC:96100JK9-3dThere are a, alpha and a/alpha diploids

36、of JK9-3d with the following genotypes:Genotypes: JK9-3da MAT a leu2-3,112 ura3-52 rme1 trp1 his4 |JK9-3d a has the same genotype as JK9 -3da with the exception of the MAT locusJK9-3da/ a is homozygous for all markers except mating typeNotes: JK9-3d was constructed by Jeanette Kunz while in Mike Hal

37、l's lab. She made the original strain while Joe|Heitman isolated isogenic strains of opposite mating type and derived the a/alpha isogenic diploid by mating type switching. It has in its background S288c, a strain from the Oshima lab, and a strain from the Herskowitz lab. It was chosen because o

38、f its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes|under control of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations. |References: | Heitman et al. (1991a) Science 253(5022):905-9 and | Heitman et al. (1991b) Proc Natl Acad Sci U