苦玄参育种材料遗传多样性SSR分析.doc

1、苦玄参育种材料遗传多样性SSR分析p 【摘要】：p ：【目的】探索苦玄參育种材料的遗传多样性及亲缘关系，为苦玄参育种资的选择及遗传改良提供参考依据。【方法】利用SSR分子标记，分析p 12份苦玄参育种材料的基因多样性指数（Nei）、Shannon多态性信息指数（I）、多态性百分率（P）及各材料间的遗传距离，并基于遗传距离对其进行聚类分析p ，以明确该育种材料的遗传多样性和亲缘关系。【结果】17对SSR引物共扩增出73个等位基因，平均每对引物4.3个。各引物间P变化范围为8.33100.00，平均60.78；多态信息量（PIC）变化范围为0.07670.7598，平均0.5184。供试材料遗传多

2、样性的平均Nei=0.3002、I=0.4162、P=59.81，其中_yj01、_yj06和ytL09种质Nei、I和P最高，分别为0.3529、0.4893和70.59；L_j12种质Nei、I和P最低，分别为0.2059、0.2854及41.18。ytl09和zgb03 2份种质间遗传距离最近，仅0.0929。基于遗传距离的聚类分析p 结果表明，在遗传距离0.4084处可将12份种质分成4个群体，其中wzhj7、l_j09和_yj01自成一群，其余归为一群。L_j12与zgb04、ytl11与wzhj9、ytl09与zgb03、wzhj_12与_yj06和wzhzp22间分别拥有较近的亲

3、缘关系。【结论】供试12份苦玄参种质材料间存在一定的遗传差异，作为育种亲本具有一定的选择价值。【关键词】：p ： 苦玄参；SSR；遗传多样性中图分类号： S567.53 文献标志码：A 文章编号：2095-1191（20_）01-0020-06Abstract：【Objective】The objective of this research was to e_plore geic diversity and geic relationship of Picria felterrae Lour.breeding materials and to provide reference for br

4、eeding materials selection and geic improvement.【Method】Using SSR molecular marker， gene diversity inde_（Nei），shannon diversity information inde_（I），percentage of polymorphic loci（P） and geic distance of 12 breeding materials P.felterrae Lour.were analyzed.And cluster analysis was conducted based on

5、 geic distance to study the geic diversity and geic relationship of the 12 breeding materials.【Result】A total of 73 alleles were lified from 17 pairs of SSR primers，4.3 alleles per primer.Among the primers， P varied between 8.33-100.00，with an average of 60.78； polymorphic information content（PIC） v

6、aried between 0.0767-0.7598 which was 0.5184 on average.For the 12 germplasm sles tested，the average level of geic diversity was Nei=0.3002，I=0.4162，and P=59.81.Number _yj01， _yj06 and ytL09 had the highest Nei which was 0.3529， the highest I 0.4893 and the highest P 70.59.Whereas L_j12 had the lowe

7、st level in Nei which was 0.2059，the lowest I 0.2854 and the lowest P 41.18.Number ytl09 and zgb03 had the shortest geic distance， which was 0.0929.The cluster analysis based on geic distance revealed that the 12 germplasm sles could be divided into four groups at the location where geic distance wa

8、s 0.4084.Number wzhj7，l_j09，and _yj01 made up three groups by themselves， and the rest was in the last group.There were close geic relationships between L_j12 and zgb04，between ytl11 and wzhj9，between ytl09 and zgb03，between wzhj_12 and _yj06， wzhzp22.【Conclusion】Certain geic difference e_ists in the