流式细胞FACS多色分析

1、23-13536-00 rev. 01multicolor analysisanalysis23-13536-00 rev. 012multicolor analysis strategies plot types hierarchical gating population hierarchies back gatinganalysis23-13536-00 rev. 013use different plot typeshistogramsusing histograms can help discriminate between two populations.dot plot: har

2、d to discriminate populationsdot plot: displaying fewer events improves discriminationhistogram: population boundary is evident analysis23-13536-00 rev. 014use different plot typescontour plotsusing contour plots can: help locate population means. indicate a need for biexponential scaling.dot plot:

3、hard to see populations bunched on axiscontour plot: shows that biexponential display might be needed biexponential contour plot: gives best display of populations analysis23-13536-00 rev. 015hierarchical gatinganalysis23-13536-00 rev. 016hierarchical gatingwhat went wrong here?correct hierarchyanal

4、ysis23-13536-00 rev. 017back gating researcher wants to discriminate tregs based on cd25 and cd127 expression. need to ensure a gate includes the entire foxp3+ population.analysis23-13536-00 rev. 018additional analysis strategies biexponential scaling events to display hinged and offset quadrant gat

5、es derived (boolean) gates snap-to gates statistics analysis templatesanalysis23-13536-00 rev. 019ics data analysis resultsinformation that can be retrieved: number of collected events, number of live cd3+ cells % of main populations: %cd3+, cd4+, cd8+ % of nave (cd45ra+) and memory (cd45ra) cd4+ and cd8+ cells % cd25+cd4+ cells (tregs) for the cytokines: % of cd4+ and cd8+maki