如何用NCBI和uniprot数据库查找目的蛋白的氨基酸序列或目的基因的碱基序列mRNA序列

1、运用NCBI数据库查找目的蛋白序列或目的碱基序列第1种方法1.打开NCBI官网，并选择Gene菜单，如下图。2 ZC8I Resources 9 How To Q2NCBINaoort3i Center (ofBFw Joj 匸小 心，/ AssemblyBoProiect Bosampie BoSystcTO Books Cinvar CloneCOfYserxGd Donans dtX3aP dbVar ESTGeneAll DatabasesSearchHCBI Homoto NCBIRoccMircQ List (A Z)All ResourcesChemicals & BoasssD

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3、maticnSubmitDownloadLearnor manusenpts3la333C5DevelopUs* NCBI APIs and code litxars to Duid 3 跑 icamngTransfer NC0 data to you* computerFind hdp documents attend a class or watch a tutondPopular ReiourcPubMedBookshWPubMod CentralBLASTGenomesnpGeneProteinPubChomAnalyzeIdEify tJCBI tool fx your data a

4、nalysis la皱ResearchNCBI AnnouncerExmore NCB learch arrf coliacooi pro；easC5V3f Jdy2016 Ca new 1000 G 的 ows xarorrts31st NCB k2在搜索栏输入想要查找的蛋白名称或基因名称，此处以基因RSK2为例，如图。Gano sources GenomeCaioaonasAnnctatedficnesPr*nworgSDqiKfco corcrxCCDSEraemslRetSeqRetScqGeneS3耐 CurrentChrTosome UxatSoM.more.CZ刖iCMTaeol

5、ar 20 曲Son Rele*ance -Send to -FiKea;F他场Pm Laai9 对汕 1 thscanlinuwl a rp4:哎d Hen*.S*reb deuiHDid you mean RSK2 as a gene srrtxa* Soatch Gone for RSK2 as 2 4*fmCclHacreGene IODescnpticnLocztian/JasesMIMKS?AllQE BFS6KA3nbesonsi ;o(em S6 Mg) A3咖 X.CIS. HU-3. ISFK-130W5ID 6107Wcn0 “HP ihunsilNO 000023 11

6、htKPWKlB. MRX19. RSK.0149911 2026714RSK2. SGK.alO e)M2corhmertPtWRSK2E加跑3nboso曲 croton S6 畅0?Chrawoe X.MAPKAPK-10. MPK-9 Re*2I SearchIO 110651FoPfpyide 3euosW20 0000&S 7S&* 10)W 0C43M 4DmetOGV596 RSK. R?k.22S2S9U 22536371)SGOI dRSK igr rsk rk2Q RSK2 AMD (3IWC匚rz6心Lnbosomii protein S6 kinaMS(MI M0 rp

7、s6*a3 Be rsk2ID 399376SOkDd poVpiHfe 3 L homeciou5 Homo sapiens rib3.在搜索结果中查找目的蛋白，注意Description栏会标明物种信息，以human为例.Al iases栏会列出目的蛋白的各种别名。Search resultsItems: 1 to 20 or 203O Sqp 合Iso 1 disuonlinugd of lEQlacfdPage 1 j of 11 NextLastNan-e/Gene IDDescriptionLocationAliasesMIMF RPS6KA3ID 6197ribosorral p

8、rotein S6 kinase A3 Homo sap心s (hurran)Chromosome X,NC 000023.11 (20149911.20267614, complement)CLS、HU3： ISPK-1, MAPKAPK1B MRX19. RSK RSK2. S6K-alpha3. p90-RSK2. po90RSK2300076页脚4在Searchresult页面中点击你要找的蛋白名称(Name/Gene ID),进入页面并在页面找到 NCBI Reference Sequences (RefSeq)条目.并在其子条目中找到amRNA and Protein(s) ”，查

9、找mRM碱基序列点击MM,查找蛋白氨基酸序列点击NP,以下以查找mRNA碱基序列为 例如下： NCBI Reference Sequences (RefSeq)B RefSeqs maintained independently of Annotated GenomesThese reference sequences exist independently of genome builds. ExplainGenomic1. NGJ07488.1 RefSeqGeneRangeDov/nload5001.121722GenBank. FASTA. Seauence Viewer (Graph

10、ics)mRNA and Proteinls1. NM 004586.2 -* NP 004577.1 ribosomal protein S6 kinase alpha-3See idwnticsl proteins and their annotated locations for NP 004577.1点击NM-004586. 2进入页面后如下：o)NCBI is phasing out sequQnca Gl numbers in Septemoer 2016. Please use accassion version! Rmd moreGenBank Homo sapiens rib

11、osomal protein S6 kinase A3 (RPS6KA3), mRNANCBI Reference Sequence: NM_004586.2FASTA GimphKSGd to LOCUSDEFINITIONACCESSION VERSION KEORDS SOUFCEOEGANI弘NM0045867723 bp mKHA linear PRI 03-KAE-2016Komo sapiens ribosonal protein S6 kinaso A3 (RPS6KA3),WL004586 XM_939339 XM_944112MLOO4586.2 GI: 56243494R

12、efSeq Komo sapiens (hgan)Homo 3 03X2Eukicyo-t a. Metazoa. Chordat a. Cianiata, Vcrtcb 匚 id a; Euteleostomi; Najiurialia、 Eutheria. Euarchonloglires; Priniates; Haplorrhini. Catarrhini; Hoiriinidae ; Homo.在页面下方会列出mRNA的序列:ORIGIN161121caaactgaag aagtcagtat caaagaaatt gcaatcacac atcatgt aaa ggaaggacat18

13、1gaaaaggcag atccttccca gtttgaactt ttaaaagtat tagggcaggg atcatttgga241aaggttttct tagttaaaaa aatctcaggc tctgatgcta ggcagcttta tgccatgaag301gtattgaaga aggccacact gaaagttcga gaccgagttc ggacaaaaat ggaacgtgat361atcttggtag aggttaatca tccttttatt gtcaagttgc attatgcttt tcaaactgaa421gggaagttgt atcttatttt ggatt

14、ttctc aggggaggag atttgtttac acgcttatcc481aaagaggtga tgttcacaga agaagatgtc aaattctact tggctgaact tgcacttgct541tt agaccatc tacatagcct gggaataatt t at agagact taaaaccaga aaat at actt601cttgatgaag aaggtcacat caagttaaca gatttcggcc taagtaaaga gtctattgac661catgaaaaga aggcatattc tttttgtgga actatatggctcc aga

15、agtagtt721781at gettactg gtacactccc tttccaagga aaagatcgaa aagaaacaat gactatgatt841cttaaagcca aacttggaat gccacagttt ttgagtcctg aagcgcagag tcttttaega901atgcttttca agcgaaatcc tgcaaacaga ttaggtgcag gaccagatgg agttgaagaa961attaaaagac attcattttt ctcaacgata gaetggaata aactgtatag aagagaaatt1021catccgccat tt

16、aaacctgc aacgggcagg cctgaagata cattctattt tgatcctgag1081tttactgcaa aaactcccaa agattcacct ggcattccac ctagtgetaa tgcacatcag1141ctttttcggg ggtttagttt tgttgctatt acctcagatg atgaaagcca agetatgeag1201acagttggtg tacattcaat tgttcagcag ttacacagga acagtattca gtttactgat1261ggatatgaag taaaagaaga tattggagtt ggct

17、cctact ctgtttgcaa gagatgtata1321cataaageta caaacatgga gtttgcagtg aagattattg ataaaagcaa gagagaccca第2中方法1 进入Uniplot数据库，在搜索栏中直接输入要查找的基因或蛋白名称，如下图:The mis引on of UniProt is to provide the scientific community with a comprehensive, high-quolity and freely acces引ble resource of piUniProtKBUniProt Knowledgeb

18、aseSwiss-Prot (551,705):刃 Manually annotatedand reviewedTrEMBL (65.378.749)Automatically annotated and not reviev/ed.riHinn artoH2 进入以下页面:UniRefSequence clustersUniParcSequence archiveProteomesSupporting dataUterature citationsTaxonomySubcellular locationss中0Cross-ref. databasesDiseasesKeywords艺力 入g

19、XYXI IniPrnf HataBLAST Aln fU(rkr./ID mopingUniProtKB results About LTiProoSFilter by丄 OozkMdX Cobrm? ：1 to of so a sfton E?1 Rflxeswd 估0)Stft-Crotfe 叶e、e“ed (0)TrBVBLPopular organismsKTftan w阴x w0O*HC Q)伽O)X0A23tKr orgjincnas；ZyE*try nameVOteifW *ORC5Did you nMdi* 依2? 3,373fco-dD 053)2K56A)_M4A1:P1

20、W54K5GA3.WUSEtftxxontal fttoln % Uiuim alptid-3MO5oml protein S Mm)x olp*o-3El019K50A2-MJMJWDKS6A2_MDUSH023482K-K01.HUhUM:陀7301和03屮|他I peaceswo 1 MouseftPQMCA3 JSPK1,KV.PKAPK18, RSK2 RpsOka? MjpMpklb.W,砂2OroismQpso (Murian)Mr rmjsoAis :叫刻Monomrtl ftret(恤 Himmj lphti-2Kbosonial fvotaln S6 hifwiMi alp

21、ha 2MitOQaivactivdttbd prooanUfMIM 1MltoQci*- actwoted protm MfMIMt 3RPWCA7 rAPKfiPKJC. Htk ?Jip*a? (Wurvan) RSK3Rp6ka2 NUpkapkic. 32MAPKi ERK2, WKM1.PRKM2MAPK3 ERK1. PKM3Hftrw Mpicnfi (Hunan)Hc*no wp*es iHunan)MitoQcnoctivatcd procn Mpkl ErtK-a丄7710/gene=,YRPS6KA3/gene_synon7TTi=rrCLS; HU-3; ISPK-1; JitAPKAPKIB; MRS 19;P0O-RSK2; pp90RSK2; RSK; RSK2; S6K-al