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基于ITS序列分析铁皮石斛试管苗的亲缘关系A1A0A3A2A4A5A6A7A8A9A10A11A5A12A13A14A15A16A17A18A19A20A21A22A23A24A25A26A27A28A29A30A31A32A33A34A35A36A37A38A39A40A41A42A43A44A45A25A24A46A47A7A20A48A49A50A25A51A4A52A38A53A54A5A6A55A56A57A2A58A59A60A28A24A15A16A29A61A5A62A53A63A64A65A66A31A67A68A69A70A71A72A73A74A75A20A76A77A78A74A79A64A65A66A80A81A82A83A84A85A82A31A4A86A87A88A89A90A77A91A92A93A35A36A7A8A9A5A12A57A2A4A94A95A11A96A97A98A99A100A11A5A12A13A14A15A16A17A18A19A101A23A47A7A31A102A59A60A28A24A15A16A103A104A105A25A72A73A74A75A82A83A35A36A5A12A31A106A24A10A11A107A108A25A7A8A109A37A110A111A112A57ITSA82A83A113A15A16A24A46A114A113A115A21A116A117A25A118A119A31A13A14A15A16A120A21A121A122A24A15A16A123A124A122A125A126A119A30A25A82A83A127A128A31A109A129A130A116A131A13A14A15A16A17A18A19A120A132A133A13A14A15A16A38A134A135A111A105A24A31A136A15A16A44A21A121A24A120A137A106A24A26A27A47A7A138A139A57A140A141A142A3A13A14A15A16A17A18A19A143A72A73A74A82A83A143A7A8A48A144A143A101A23A47A7A145A146A147A148A3A149A150A151A149A152A153A154A155A152THEPHYLOGENETICRELATIONSHIPSOFTISSUECULTURESEEDLINGSOFDENDROBIUMCANDIDUMBASEDONEVIDENCESFROMITSSEQUENCESABSTRACTA156MOLECULARSYSTEMATICTECHNIQUESWEREAPPLIEDTOREVEALTHEGENETICDIVERSITYOFMEDICINALPLANTOFDENDROBIUMCANDIDUMANDTHEIRRELATEDSPECIESINDENDROBIUMTHEINTERNALTRANSCRIBEDSPACERITSASWELLAS58SRDNASEQUENCESOFMORETHAN30SAMPLESOFDENDROBIUMWEREAMPLIFIEDUSINGPCRMETHODANDSEQUENCEDA157MEGA31WASUSEDTOANALYZETHEGENETICDIVERSITYWITHINGENUSPHYLOGENETICRELATIONSHIPSAMONGSPECIESINDENDROBIUMANDTISSUECULTURESEEDLINGSOFDENDROBIUMCANDIDUMASOUTGROUPWEREESTIMATEDBYMAXIMUMPARSIMONYANDNEIGHBORJOININGMETHODSWITHMEGA31SOFTWAREA158ANDSIMILARITSTREESWEREOBTAINEDWITHTHETWOMETHODSTHERESULTSOFPHYLOGENETICANALYSISALSOINDICATETHATTHETISSUECULTURESEEDLINGSOFDENDROBIUMCANDIDUMHASACLOSEDRELATIONSHIPWITHDENDROBIUMCANDIDUM,WHILEBOTHDENDROBIUMCANDIDUMANDTISSUECULTURESEEDLINGSOFDENDROBIUMCANDIDUMARETHEMOSTDISTANTTOTHEOTHERSPECIESOFDENDROBIUMKEYWORDSA159TISSUECULTURESEEDLINGSOFDENDROBIUMCANDIDUMA160ITSSEQUENCEA160PHYLOGENYA160RELATIONSHIPA161A162A163A164A165DENDROBIUMCANDIDUMWALLEXLINDLA166A167A168A169A170A165ORCHIDACEAEA166A163A164A171A165DENDROBIUMSWA166A172A173A174A175A176A177A167A178A179A180A181A182A183A184A185A186A187A188A167A168A163A164A187A189A190A167A191A192A193A194A195A196A197A175A198A199A200A197A201A202A203A204A205A206A207A208A186A209A210A211A212A213A161A162A163A164A178A188A214A215A192A216A217A218A185A186A214A219A220A221A222A22312A167A224A225A189A226A227A228A218A229A230A231A232A233A234A235A236A237A238A239A240A218A241A242A243A239A208A244A245A161A162A163A164A210A211A218A246A247A248A249A167A250A251A252A161A162A163A164A218A253A254A226A246A247A221A255A208A98A173A0A100A1A102A2A3A4A5A6A7A8A203A8A109A111A9A10A167A114A11A12A13A206A167A118A14A12A15A16A218A17A18A19A20A21A22A23A229A208A186A229A109A28A24A25A229A6A7A8A203A205A26A27A3A4A22A76A29A130A30A31A32A33A34A35A36A137A37A38A39A40A41A42A43A44A45A46A76A47A32A48A49A36A41A140A50A51A52A53A54A55A56A57A58A59A42A39A60A36A68A56A76A29A61A62A32A63A64A65A66A67A86A69A70A71A42A72A73A74A32A75A77A59A78A36A163A79A80A70A71A157A81A73A74A130A35A82A168A83A54A55A32A170A84A171A172A85A40A173A87A132A78310A67A137A37A174A163A79A80A32A175A88A89A178A90A91A92A93A87A32A94A95A39A96A97A99A36A130A101A103A104A91A92A93A105A32A106A107A52A36A191A192A108A193A110A112A113A115A116A117A119A84A37A120A72A121A122A123A124A122A125A126A61A125A32A127A128A67ITSA65A129A131A133A134A115A71A120A72A125A126A123A132A60A127A128A35A32A135A136A70A71A157A81A36A205A136A95A37A131A138A139A141A54A55A142A191A210A211A66A36A212A13418S226SRDNAA32A66A141A143A133A144A70A36ITSA130A145A95A191A143A35A56A146A103A135A147A32A36A217A148A149A33A150A53A151A152A46A95A191A153A152A36A222A154A135A147A155A156A122A171A59A42A40A156A158A159A160A156A158A122A32A161A162A132A60A36A222A164A134A165PCRA232A166A167A72A169A176A177A179A180A83A40A181A182A95A183A184A211A185A36DNAA177A179A236A212A32A186A187A34A103A188A133A189A123DNAA245A190A101A103A92A194A195A61A125A36A249A71A120A72ITSA178A32A101A103A39A60A196A197A198A199A36A217A115A71A120A72A32ITSA178A101A103A200A201A93A202A83A36A203A20458SRDNAA130A159A36A206A101A103A207A92600A208700BPA36A134A177A179A209A113A40A198A199A54A213A67A189A127A128A214A84RDNAA32ITSA165A215A216A121A120A72A32A179A218A132A219A201A93A70A220A36A221A130A223A224A41A124A122A32A140A50A39A96A131A188A225A29A65A122A32A226A227A61A125A36A134A228A229A215A216A230A231A233A125A126A234A127A128A235A237A238A239A671材料和方法11A240A241A242A243A240A241A242A243A244A246A247A248A250A251A252A253A254A255A127A23A0A11A2A4A3A5A6A7A8A9A10A11A12A13A14A15A17A16A2A140A11A26A18A4A3A5A6A19A27A131A28A20A21A39A69A31A47A22A24,A32A25A5A6A29A28A72A21A30A2A5A6A140A33A199A34A35A131A28A72A20A21A50A361A39A37A38A40A41A42A29A43TABA1411ORIGINANDN01TTE0FPLANTMATERIALSA142A44A144A45A46A48A142A44A144A45A46A48A142A44A144A45A46A481DDEVONIANUMA148A49A51A52A1502DDEVONIANUMA148A49A51A52A1513DSTRONGYLANTHUMA152A53A51A524DCRYSTALLINUMA154A54A51A52A1505DCRYSTALLINUMA154A54A51A52A1516DADUNCUMA156A55A51A527DMONILIFORMEA157A56A51A52A1508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