DB32T 4056-2021 优良食味半糯粳稻品质

DB32Qualityofsemi-glutinousjaponicaricewithgoodtaste江苏省市场监督管理局发布I宋锦花、魏海燕、赵春芳、魏晓东、陈涛、赵庆勇、赵凌、姚姝、周丽慧、路凯、梁文12规范性引用文件GB/T5491粮食、油料检验扦样GB/T21719稻谷整精米GB/T22294粮油检验大米胶稠度NY/T593食用稻品种品质NY/T2639稻米直链淀粉的测定分光光度法半糯粳稻semi-glutinousjaponicarice优良食味半糯粳稻semi-glutinousjaponicaricewithgoodtaste2鉴别标记identificationmolecular4质量要求4.1基本质量要求4.2定等质量指标一二三整精米率/%123胶稠度/mm5.5透明度检验：按照NY/T831DB32/T4056-2021A.1原理2A.3.4DNA回收试剂A.5分子标记序列正向外引物（Wxmq/Wxmp-O-F）序列为5A.5.2用于鉴定Wxmw的分子标记序列3A.8.1琼脂糖凝胶制备A.8.2上样样品制备A.8.4凝胶成像将电泳后的琼脂糖凝胶放于凝胶成像系统的紫外光源下进行PCR扩增产物观察，拍照备用。对引物Wxmq-F/R扩增得到的PCR条A.9结果分析A.9.1分子标记检测结果分析利用四引物Wxmq/Wxmp-O-F/O-R/I-F/I-R分4和301bpDNA条带的样品，证明含Wx"突变位点；扩增得到459b 第5外显子1:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAIACAAAGACAACCAGATGCGTITCAGCCTTCTTT2:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATCACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGC3:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAITACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG4:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAIACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG5:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAIACAAAGACAACCAGATGCGTITCAGCCTTCTTTGCCAG6:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG7:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAIACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG8:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG9:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATTACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG10:GTITGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGAITTACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG11:GTTTGGGGAAAGACCGGTGAGAAGATCTACGGACCTGACACTGGAGTTGATT/dAACAAAGACAACCAGATGCGTTTCAGCCTTCTTTGCCAG12:GTTTGGGGAAAGACCGGTGAGAAGATCTACCGACCTGACACTGGAGTTOATticAC