微流体芯片资料

微流体芯片资料第1页/共31页ContentsBackgroundExperimentABSTRACTDiscussion第2页/共31页ABSTRACTMicrodropletsofferuniquecompartmentsforaccommodatingalargenumberofchemicalandbiologicalreactionsintinyvolumewithprecisecontrol.Amajorconcernindroplet-basedmicrofluidicsisthedifficultytoaddressdropletsindividuallyandachievehighthroughputatthesametime.第3页/共31页Here,wehavecombinedanimprovedcartridgesamplingtechniquewithamicrofluidicchiptoperformdropletscreeningsandaggressivereactionwithreagentconsumption.Ourcombineddeviceprovidesasimplemodeltoutilizemultipledropletsforvariousreactionswithlowreagentconsumptionandhighthroughput.第4页/共31页第5页/共31页BackgroundAlargenumberofreactionscanbeindependentlyminiaturizedintotinydropletsrangingfromnanolitertopicolitersizeswithouthavingtoincreasethechipsizeandcomplexity.

Microfluidicchipshaveprovidedapowerfulplatformforaccomplishingdropletformationandmanipulationwithinonechipofonlyafewsquarecentimetersinsize.

AdvantagesOverthepastfewyears,droplet-basedmicrofluidicshasattractedincreasingattention.第6页/共31页Background

Dropletgenerationformultiplesamplesislimitedbythe“world-to-chip”interfaceproblemandcannotbeaddressedindividuallyDropletgenerationandmanipulationfunctionswithinonechipcaneasilyinterferewitheachotherandaredifficultTobalance,despitebeingseparatedbyalong,windingchannel.DrawbackPassivedropletformationmethods:第7页/共31页InnovateThiscontextshowsahybriddevice:thecartridgedropletgenerationtechniquecombinedwithamicrofluidicchip.Theyimprovedthecartridgetechniquewithanovelmovementscheme:thecapillary,insteadofthemultiwellplate,wasmovedbyadigitalandautomaticmanipulatortoaspiratedifferentsamplesorthecoveringoil.第8页/共31页Thehybriddropletdeviceforgenerationandmanipulationconsistsoffourmajorcomponents.

(a)manipulator(b)multiwellplate(c)capillary(d)PDMSchip第9页/共31页第10页/共31页第11页/共31页Threegeneralsteps

loadthemultiwellplatewithdifferentsamples,coverwithalayerofoil,andinputtheparametersofthedesireddropletarrayintothecontrollerviaaPC

startthecontrollertoaspiratecertainvolumesofsamplesandoilintotheCapillarysequentially

introducethegenerateddropletsintothePDMSchipformerging,mixing,detection,orothermanipulation.第12页/共31页Laboratoryreagent

siliconeoil,

FeCl2·4H2O(Sigma−Aldrich),FeCl3·6H2O(AcrosOrganics),ammoniumhydroxidesolution(28%NH3Fluka),andhydrochloricacid(37%HCl,AcrosOrganics)第13页/共31页ImprovedSamplingTechnique

CombinedDropletDevicePerformance

High-ThroughputDropletScreening

AggressiveDropletReaction

第14页/共31页ImprovedSamplingTechniqueThecapillaryaspiratestheoilwhenthecantileverremainsinthe“up”state.Whenthecantileveristriggeredtostayinthe“down”state,thecapillaryaspiratesthesample.Theresidencetimeintheoil(TO)andsample(TS)couldbeadjustedrespectivelytogeneratedropletswithdesireddistanceandvolume.第15页/共31页第16页/共31页(c)Relativeintensitywasmeasuredalongthechannelstartingfromtoprightcorner.TheintensityoffluoresceinandDIwaterdropletdoesnotdecreaseorincrease,whichindicatesthatnocross-contaminationbetweenthesetwosampleswasdetected.(b)Dropletarraywasgeneratedbysequentiallyaspirating50uMfluoresceinandDIwater.第17页/共31页CombinedDropletDevicePerformanceHere,themergingofdropletswasdemonstratedinaPDMSchip.ThedropletsfusiondoesnotdependondropletdistancebutdropletvelocityandVd/Vc.Aftermixingcompletely,themergeddropletwasoutputtoasinuouschannelfordisplayanddetection.第18页/共31页第19页/共31页Inthefirstapplication,aseriesofAiandBm,n(i=1−2,m=1−3,andn=1−6)weregenerated.A1andA2areFluo-4andRhod-2solutionswiththesameconcentration(10.8μM),whichareindicatorsthatexhibitanincreaseinfluorescence(green/red)uponmetalionbonding.Avalueofm=1−3representmetalsionsolutionsofLaIII,YbIII,andYIII,respectively,whilen=1−6aresixdifferentmetalionconcentrations(0,2.5,5,10,20,and40μM)ofthesesolutions.Thedropletarraycontains721-nLdroplets第20页/共31页High-ThroughputDropletScreening第21页/共31页第22页/共31页The36pairsofdropletreactionsused∼72nLofsolutionandrequired1.8mintocomplete.Therefore,thereagentconsumptionforeachmeasurementcouldbereducedto1nLpersample,andthereactionthroughputis1200h−1,whichcanbemuchfasterbyincreasingtheflowvelocity.ThescreeningdropletscouldbestoredinthePDMSchipafterwestoppedthevacuum.第23页/共31页AggressiveDropletReaction第24页/共31页Fromtheseimages,wefoundthatthecoprecipitationissofastthatitimmediatelyformsparticleswhentwodropletscomeintocontactwitheachother.Moreover,ineachvolumeratio,theprecipitationinitiatedbythefusionoftwodropletsdidnotblockthemicrochannelatsuchhighconcentrations.Suchflexible,controllableandminiaturizedreactionconditionsareextremelysuitableforaggressive,fast,orevenexplosivereactionsthatgenerateprecipitationorheat.第25页/共31页DiscussionThecartridgedropletgenerationmethodprovidesanefficientwayforintroducingdifferentnanoliterdropletsintothemicrofluidicchip.themicrofluidicchipoffersaplatformfordelicatemanipulationsuchasmerging.Thisfeatureisvaluableforreducingthereactionscreeningandoptimizationcostandtimeandisparticularlyusefulfordrugscreening.第26页/共31页Comparedwiththeothercartridgedropletgeneration,ourdesignusedadigitalandautomaticmanipulatortomovethecapillaryinsteadofthemultiwellplate.DiscussionSubsequently,thecoveringoilwasingeniouslyuseda