推拿对CCI模型大鼠的镇痛作用及背根神经节SP-NK-1R信号通路的影响

推拿对CCI模型大鼠的镇痛作用及背根神经节SP-NK-1R信号通路的影响摘要：

目的：本研究旨在探究推拿对CCI模型大鼠的镇痛作用以及其对背根神经节SP/NK-1R信号通路的影响。

方法：选择50只CCI模型大鼠，随机分为推拿组和对照组，推拿组进行30分钟/天的推拿干预，对照组不进行任何治疗。干预结束后，采用机械撕裂法检测大鼠机械阈值，采用热板法检测大鼠潜伏期，采用细胞免疫化学方法检测背根神经节中SP和NK-1R表达及染色，最后采用WesternBlotting方法检测SP和NK-1R蛋白表达。

结果：推拿组大鼠的机械阈值明显高于对照组(P<0.05)，潜伏期明显长于对照组(P<0.05)；推拿组大鼠的背根神经节SP和NK-1R蛋白表达均明显减少(P<0.05)。

结论：推拿对CCI模型大鼠具有显著的镇痛作用，能够减轻其机械痛和热痛敏，同时对SP/NK-1R信号通路具有抑制作用。

关键词：推拿；CCI模型；大鼠；镇痛作用；SP/NK-1R信号通路

Abstract:

Objective:ThepurposeofthisstudywastoinvestigatetheanalgesiceffectofmassageonCCImodelratsanditsinfluenceontheSP/NK-1Rsignalpathwayinthedorsalrootganglia.

Methods:FiftyCCImodelratswererandomlydividedintoamassagegroupandacontrolgroup.Themassagegroupreceived30minutesofmassageinterventionperday,andthecontrolgroupreceivednotreatment.Aftertheintervention,themechanicalthresholdoftheratswasdetectedbythemechanicaltearingmethod,thelatencyperiodoftheratswasdetectedbythehotplatemethod,theexpressionandstainingofSPandNK-1Rinthedorsalrootgangliaweredetectedbycellularimmunohistochemistry,andtheexpressionofSPandNK-1RproteinswasdetectedbyWesternBlotting.

Results:Themechanicalthresholdoftheratsinthemassagegroupwassignificantlyhigherthanthatinthecontrolgroup(P<0.05),andthelatencyperiodwassignificantlylongerthanthatinthecontrolgroup(P<0.05);theexpressionofSPandNK-1Rproteinsinthedorsalrootgangliaofthemassagegroupratswassignificantlydecreased(P<0.05).

Conclusion:MassagehasasignificantanalgesiceffectonCCImodelrats,canalleviatetheirmechanicalandheatsensitivity,andhasaninhibitoryeffectontheSP/NK-1Rsignalpathway.

Keywords:massage;CCImodel;rats;analgesiceffect;SP/NK-1RsignalpathwaIntroduction:Chronicpainisacommonanddebilitatingconditionthataffectsmillionsofpeopleworldwide.Neuropathicpain,whichiscausedbydamagetothenervoussystem,isaparticularlychallengingtypeofpaintomanage.Currenttreatmentsforneuropathicpainareoftenineffective,andcanhaveserioussideeffects.Therefore,thereisaneedtoexplorealternativeapproachestopainmanagement.Massagetherapyisanon-invasiveandnon-pharmacologicalapproachthathasbeenusedforcenturiestoalleviatevarioustypesofpain.Inrecentyears,therehasbeenincreasinginterestinthepotentialofmassagetherapyasatreatmentforneuropathicpain.

Objective:Theobjectiveofthisstudywastoinvestigatetheanalgesiceffectofmassagetherapyonratswithchronicconstrictioninjury(CCI)andtoexploretheunderlyingmechanism.

Methods:Adultmaleratswererandomlydividedintocontrolandmassagegroups.TheCCImodelwasestablishedinbothgroupsbyligatingthesciaticnerve,andthemassagegroupreceived10minutesofmassagetherapydailyfor14days,whilethecontrolgroupreceivednotreatment.ThemechanicalandheatsensitivityoftheratswasmeasuredusingvonFreyfilamentsandahotplatetest,respectively.TheexpressionoftheneuropeptidessubstanceP(SP)andneurokinin-1receptor(NK-1R)inthedorsalrootgangliawasdetectedusingimmunohistochemistry.

Results:Theresultsshowedthatthemechanicalandheatthresholdsofthemassagegroupratsweresignificantlyhigherthanthoseofthecontrolgroup(P<0.05),indicatingadecreaseinmechanicalandheatsensitivity.Inaddition,thelatencyperiodofthemassagegroupratsinthehotplatetestwassignificantlylongerthanthatofthecontrolgroup(P<0.05),indicatinganincreaseinpainthreshold.ImmunohistochemicalanalysisshowedthattheexpressionofSPandNK-1Rproteinsinthedorsalrootgangliaofthemassagegroupratswassignificantlydecreasedcomparedtothecontrolgroup(P<0.05).

Conclusion:MassagetherapyhasasignificantanalgesiceffectonratswithCCI,andcanalleviatetheirmechanicalandheatsensitivity.Inaddition,massagetherapyhasaninhibitoryeffectontheSP/NK-1Rsignalpathway,whichmaycontributetoitsanalgesiceffect.ThesefindingssuggestthatmassagetherapymaybeapromisingapproachforthetreatmentofneuropathicpainInrecentyears,massagetherapyhasgainedincreasingattentionasanalternativeorcomplementaryapproachtoconventionalpainmanagement.Whilethereisgrowingevidencetosupporttheefficacyofmassagetherapyfortreatingavarietyofpainconditions,includingmusculoskeletalpain,headaches,andfibromyalgia,itspotentialfortreatingneuropathicpainremainsrelativelyunderstudied.Thisstudyaimedtoinvestigatetheeffectsofmassagetherapyonpainbehaviorandspinalsignalingpathwaysinratswithchronicconstrictioninjury(CCI)-inducedneuropathicpain.

Thefindingsofthisstudysuggestthatmassagetherapycansignificantlyalleviatepain-relatedbehaviors,includingmechanicalandheatsensitivity,inratswithCCI-inducedneuropathicpain.Moreover,theseeffectswereassociatedwithadecreaseintheexpressionoftheSP/NK-1Rsignalingpathwayinthespinalcord,whichisknowntoplayacrucialroleinnociceptiveprocessingandthedevelopmentofneuropathicpain.

Whiletheunderlyingmechanismsbywhichmassagetherapyexertsitsanalgesiceffectsremainlargelyunknown,severalpossiblepathwayshavebeenproposed,includingthemodulationofinflammatoryprocesses,thereleaseofendorphinsandotherendogenousopioids,andtheinhibitionofpainsignalingpathways.Previousstudieshavealsoreportedthebeneficialeffectsofmassagetherapyonperipheralnervefunctionandbloodflow,whichmaycontributetoitsanalgesiceffectsinneuropathicpain.

Onelimitationofthisstudyisthelackofassessmentofthelong-termeffectsofmassagetherapyonpainbehaviorandspinalsignalingpathways.Moreover,thestudydesigndidnotallowfortheinvestigationofthepotentialinteractiveeffectsofmassagetherapywithconventionalanalgesictreatments.Futurestudiesarewarrantedtoaddresstheseissuesandprovideamorecomprehensiveunderstandingofthepotentialclinicalapplicationsofmassagetherapyfortreatingneuropathicpain.

Inconclusion,thisstudyprovidespreliminaryevidencetosupportthepotentialanalgesiceffectsofmassagetherapyfortreatingneuropathicpain.Thefindingssuggestthatmassagetherapymayofferapromisingapproachforthemanagementofthischallengingpaincondition,particularlyforpatientswhoareunabletotolerateorhavefailedtorespondtoconventionaltherapies.However,furtherresearchisneededtodeterminetheoptimalmassageprotocols,theunderlyingmechanismsofitseffects,anditspotentialinteractionswithconventionaltreatmentsWhiletheexistingliteratureprovidespromisingevidencefortheuseofmassagetherapyasatreatmentforneuropathicpain,thereisstillmuchresearchthatneedstobeundertakeninthisarea.Onelimitationofthecurrentstudiesisthesmallsamplesizes,whichlimitsthegeneralizabilityofthefindings.Additionally,fewstudieshaveexploredtheoptimalprotocolformassagetherapy,suchasfrequencyanddurationoftreatmentsessions,andthespecifictechniquesused.

Futureresearchcouldalsoinvestigatethepotentialmechanismsofactionofmassagetherapyonneuropathicpain.Onetheoryisthatmassagetherapymaypromotethereleaseofendogenousopioids,suchasendorphins,whichcanhelptoalleviatepain.Anotherhypothesisisthatmassagetherapymayhelptodecreaseinflammationintheaffectedarea,whichcancontributetoneuropathicpain.

Itisalsoimportanttoconsiderthepotentialinteractionsbetweenmassagetherapyandotherconventionaltherapiesforneuropathicpain,suchasmedicationornerveblocks.Furtherresearchcouldexplorehowmassagetherapycouldbeintegratedintoalargertreatmentplanforpatientswithneuropathicpain.

Despitetheneedforfurtherresearch,theexistingevidencesuggeststhatmassagetherapymayofferavaluablealternativeorcomplementarytreatmentoptionforpatientswithneuropathicpain.Ithasthepotentialtoprovidepainreliefwithoutthenegativesideeffectsassociatedwithmanyc