生化必备-2003年交大期末试卷

交通大学生物化学试卷（2003校

系

专 学 日 2003 PAPER(1)（闭卷部分(ShanghaiJiaoTongUniversity,1sttermofUniv.yearSubject: Name Class

Score 一Explaintheterms:（2.5x10=LDLpyruvatedehydrogenaseglucokinase(inmalate-aspartatesubstrate“macroureaUDP-2003 PAPER2)（开卷(ShanghaiJiaoTongUniversity,1sttermofUniv.yearSubject: Name Class

Score 二Fillingtheblanks2.5x12=Manyimportantcoenzymes,suchas,and,arethederivativesofADP;dNDParesynthesizedfromanddTMPisformedby ofdUMP;allthesefactsreflectsthatcamebefore inevolution.TPPisthederivativeofvitamin,itservesastheprostheticgroupofthefollowingenzymes: , Thereisalargedifferencebetweentheyieldfromthecompleteoxidationoftriacylglycerolsandofcarbohydrates(andprotein),thereasonis Thethreepairsofenzymesthatcatalyzethethreeresemblestepsinβ-oxidationofFAandcitricacidcycleare , catalyzedby and , and Inthesynthesisofphosphoglycerides磷酸甘油酯andofglycogen,theactivatedintermediatesare ,bothofthemareformedfroma substrateanda ,andthenreactwitha Thecommittedstepincholesterolbiosynthesisisthesynthesis from whichiscatalyzedby Thecarrieroftheactivatedone-carbonunits whichofthree

,

Besides, isthemajordonorofmethylgroup.Thefully

Diseaseandinheriteddefectsofenzymeorfunctionalprotein,e.g.PKU—hydroxylase;drug-inducedhemolyticanemia溶血性贫血— hypercholesterolemia高胆固醇血症— 性贫血— ;maplesyrup槭糖浆urinedisease ;Leber’sopticneuropathy视神经症 AcetylCoAcarboxylasefromE.coliconsistsofthreesubunits , and Therearetwotypesofcovalentmodification: intheregulationofenzymaticactivity,thetypicalexampleofwhichis and (enzyme),thedirectrelatedresidueis and ,respectively.三．Makechoiceoneorseveral)：(3x8=SchiffbaseintermediatesareformedinthereactionscatalyzedA. B.glycogen C.Trp D.E.Multifunctional(orbifunctional)enzymeoccurspyrimidinesynthesisofeukaryotesb.FAsynthesisofeukaryotesC.thesynthesisandhydrolysisofF-2,6-BPD.thephosphorylationanddephosphorylationofisocitrateDHE.FAsynthesisofE.coliPRPPparticipatesinthesynthesisofthefollowingA.ProB.HisC.purineD.PheE.Biotin,asthepostheticgroupofthefollowingacetylCoAcarboxylaseB.transketolaseC.pyruvateD.PyrDHE.α–KGThefollowingcomplexcontainsFe-SA.NADH-QredueB.cytochromecredueC.cytochromecoxidaseD.succinate-QredueE.ribonucleotideredueWhichsubstanceisthedirectprecursorofthesynthesisofacetoacetate乙酰乙酸inliverHMGCoAB.acetoacetylCoAC.D-3-hydroxybutyrateD.D-3-hydroxybutyrylCoAE.Citricaciditcanproducehigh-energyphosphorylcompoundbyitselfB.itisnotinhibitedbytheanaerobic厌氧conditionC.thetwocarbonatomsofacetylCoAareliberatedastheformoftwoCO2inthatroundofthecycleD.itsratedependsupontheneedofATPE.itisthefinalcommonpathwayfortheoxidationoffuelmolecules.Itparticipatesintheactivatedmethylcycle(orS-AMetA.VB12 B.biotin C.FH4 D.VB2 Enicotinate烟酸四.Answerorcalculate:(7x3=21)WhatistheactivatedreactantineachoftheseSphingomyelin鞘磷脂fromceramideMetfromhomocysteinePhosphatidylcholinefromphosphatidylethanolamine乙IPPfrom5-pyrophosphomevalonateThesynthesisofphosphoribosylamineSerfromGlycogenn+1fromglycogenInpurinebiosynthesis,Asp eslinkedtothecarboxylategroupofanintermediate.Inthenextreaction,thecarbonskeletonoftheAspunitleavesintheformoffumurate延胡索酸.ThesereactionsarereminiscentofasimilarpairofreactionsinthedegradationofAA.Whichones?Currencyexchange.Foraproton-motiveforceof0.2V(matrixnegative),whatisthe Calculatethisratiothreetimes,assumingthatthenumberofprotonstranslocatedperATPformedis2,3,and4andthatthetemperatureis250C.2003TESTPAPER1)一hyperuricemiaLDLreceptorB相结合的细胞表面受体。可使LDL从血浆(发生细胞)LDL的合成从而调节血浆胆固醇的浓度。pyruvatedehydrogenasecomplex酸脱氢酶复合体?催化酸、NAD+和辅酶A之间A、NADHCO2(整个反应)的酶复合体。包含三个活性酶和五个辅酶。4.glucokinase(inliver)葡萄糖激酶哺乳动物肝赃中特有的一种己糖激酶的同工酶,它与底物G-6-P的抑制，故可大量地吸纳血液中(过多)的葡萄糖。malate-aspartateshuttle苹果酸-(NADH中的电子)从从肝PRPP磷酸核糖焦磷生物合成多种含氮环状化合物极其衍生物中必不可少的反应物，特substratecycle:F-6-PF-1,6-P的互“macroCoA”巨”辅酶A”;指ACP(酰基载体蛋白),它含有与辅酶A相同的磷酸泛酰巯基乙胺头部(与脂肪酸合成过程中各中间物连接);77个氨ureacycle尿素循环;一组循环反应,其中由氨基酸代谢产物两个氨基和一个二氧化碳生UDP-glucose尿苷二磷酸葡萄糖;一种核苷二磷酸糖,作为糖原合成(和其它许多合成)2003TESTPAPER2)二B1,pyruvateDHcomplex,α-KGDHcomplex,酮脂酰-ACP还原酶,DH;羟脂酰-ACP脱水酶,延胡索酸酶;烯脂酰-ACP还原酶,DH高半胱氨酸甲基化形成甲硫氨酸,L-CoACoA,高半胱氨酸甲基转移酶,CoA变位酶柠檬酸循环形成柠檬酸,糖异生形成葡萄糖,转氨形成天冬氨酸,脱羧形成UDP-葡萄糖CDP-DAG,磷酸化,核苷三磷酸,MVA,3-HMGCoA,3-HMGCoA四氢叶酸,蝶啶,对氨基苯甲酸,谷氨酸S-腺苷甲硫氨酸,二氧化碳,10.G-6-PDH,LDL受体,内因子,DH,NADH-Q生物素羧基载体