DL-Buthionine-S-R-sulfoximine-Buthionine-sulfoximine-DataSheet-生命科学试剂-MedChemExpressVIP

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEDL-Buthionine-(S,R)-sulfoximineCat.No.:HY-106376CASNo.:5072-26-4Synonyms:Buthioninesulfoximine;BSO分⼦式:C₈H₁₈N₂O₃S分⼦量:222.31作⽤靶点:Ferroptosis作⽤通路:Apoptosis储存⽅式:Powder-20°C3yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验H2O:25mg/mL(112.46mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制备储备液1mM4.4982mL22.4911mL44.9822mL5mM0.8996mL4.4982mL8.9964mL10mM0.4498mL2.2491mL4.4982mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：PBSSolubility:100mg/mL(449.82mM);Clearsolution;Needultrasonic1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEBIOLOGICALACTIVITY⽣物活性DL-Buthionine-(S,R)-sulfoximine⾕氨酰半胱氨酸合成酶⽣物合成(glutamylcysteinesynthetasebiosynthesis)的有效抑制剂。IC50&Targetglutamylcysteinesynthetase[1]体外研究Buthioninesulfoximineisananalogsofmethioninesulfoximineandinhibitsgamma-glutamylcysteinesynthetaseabout20timesmoreeffectivelythanprothioninesulfoximineandatleast100timesmoreeffectivelythanmethioninesulfoximine[1].体内研究TreatmentofmicebearingHT1080andHT1080/DR4xenograftswithacontinuousi.vinfusionofnontoxicdosesofD,L-Buthionine-(S,R)-sulfoximine(300and600mg/kg/day)producea60%reductionofGSHplasmalevelsandgreaterthan95%reductioninGSHtumorlevelsinbothparentalandmultidrug-resistanttumors[2].PROTOCOLAnimalMice[2]Administration[2]D,L-Buthionine-(S,R)-sulfoximineisdissolvedinsterile0.9%saline,filteredthrougha0.2-p.mpolysulfonemembranefilter,andadministeredby48-hcontinuousiv.infusionatadoseof300mg/kg/dayand600mg/kg/daystartingat24hbeforedoxorubicinadministration.InvivoGSHlevelsaftertreatmentwithD,L-Buthionine-(S,R)-sulfoximineatadoseof300mg/kgand600mg/kgfor24hasaniv.continuousinfusioninmunineplasmaandintumortissueofHT1080andHT1080/DR4xenograftsismeasured[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•NucleicAcidsRes.2020Sep18;48(16):9109-9123.•Small.2021Nov1;e2103984.•EcotoxicolEnvironSaf.2022,247:114263.•EcotoxicolEnvironSaf.2022Dec1;247:114263.•MolCellBiochem.2021Jan8.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].GriffithOW,etal.Potentandspecificinhibitionofglutathionesynthesisbybuthioninesulfoximine(S-n-butylhomocysteinesulfoximine).JBiolChem.1979Aug25;254(16):7558-60.[2].VanhoeferU,etal.d,l-buthionine-(S,R)-sulfoximinepotentiatesinvivothetherapeuticefficacyofdoxorubicinagainstmultidrugresistanceprotein-expressingtumors.ClinCancerRes.1996Dec;2(12):1961-8.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEMcePdfHeightCaution:Producthasnotbeenfullyvalidat