BAPTA-AM-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEBAPTA-AMCat.No.:HY-100545CASNo.:126150-97-8分⼦式:C₃₄H₄₀N₂O₁₈分⼦量:764.68作⽤靶点:PotassiumChannel作⽤通路:MembraneTransporter/IonChannel储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:50mg/mL(65.39mM;Needultrasonic)H2O:<0.1mg/mL(ultrasonic)(insoluble)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.3077mL6.5387mL13.0774mL5mM0.2615mL1.3077mL2.6155mL10mM0.1308mL0.6539mL1.3077mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.5mg/mL(3.27mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:2.5mg/mL(3.27mM);Suspendedsolution;Needultrasonic3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(3.27mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性BAPTA-AM⼀种可透过细胞膜的钙螯合剂(Ca2+chelator)。在HEK293细胞中，BAPTA-AM阻断hERG，hKv1.3和hKv1.5通道，IC50分别为1.3，1.45和1.23μM[1]。IC50&TargetCa2+chelator[1]IC50:1.3μM(hERGchannel,inHEK293cells),1.45μM(hKv1.3,inHEK293cells),1.23μM(hKv1.5,inHEK293cells)[1]体外研究BAPTA-AMinhibitsneuronalCa2+-activatedK+channelcurrents,andup-regulatesthedecreasedcardiacsodiumcurrent(INa)densitybychelatingintracellularCa2+[1].BAPTA-AM(BAPTA/AM),anintracellularcalciumchelator,inducesdelayednecrosisbylipoxygenase-mediatedfreeradicalsinmousecorticalcultures.BAPTA-AMpreventsfreeradical-mediatedtoxicitypromoteapoptosisinnon-neuronalcellsandproduceabeneficialeffectinneuronalcellsbyprotectingneuronsfromischemicdamage.Inaddition,ithasbeensuggestedthatBAPTA-AMinducesalate,butnotearly,increaseofintracellularcalciuminI-IL-60neoplasticcells.Mixedcorticalcellcultures(DIV13-16)exposedto10μMBAPTA-AMfor24-or48-hrshowmoderate(45-70%)neuronalinjuryasevaluatedbyincreasedLDHreleaseintothebathingmediumafter24-48-hr.Exposureofcorticalculturesto3-10μMBAPTA-AMfor48-hrevokedose-dependentneuronaldamage[2].PROTOCOLCellAssay[1]Neuronalinjuryisquantitativelyestimatedbymeasuringlactatedehydrogenase(LDH)releasedfromdamagedcellsintothebathingmedium24-or48-hrafterthe10μMBAPTA/AMtreatment.Themorphologicalfindingsareconfirmedbystainingwithneuron-specificenolase(NSE)antibodyandtryphanblue[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•SignalTransductTargetTher.2022Feb16;7(1):46.•NatImmunol.2019Apr;20(4):433-446.•CellStemCell.2022Oct12;S1934-5909(22)00417-9.•NatCommun.2021May18;12(1):2915.•JExtracellVesicles.2022Oct;11(10):e12274.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESeemorecustomervalidationsonwww.MedChemEREFERENCES[1].WieMB,etal.BAPTA/AM,anintracellularcalciumchelator,inducesdelayednecrosisbylipoxygenase-mediatedfreeradicalsinmousecorticalcultures.ProgNeuropsychopharmacolBiolPsychiatry.2001Nov;25(8):1641-59.[2].TangQ,etal.ThemembranepermeablecalciumchelatorBAPTA-AMdirectlyblockshumanethera-go-go-relatedgenepotassiumchannelsstablyexpressedinHEK293cells.BiochemPharmacol.2007Dec3;74(11):1596-607.McePdfHeightCaution:Prod