3-Oxo-5β-cholanoic-acid-Dehydrolithocholic-acid-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemE3-Oxo-5-cholanoic acidCat. No.: HY-125801CAS No.: 1553-56-6Synonyms: Dehydrolithocholic acid; 3-oxoLCA分式: CHO分量: 374.56作靶点: ROR作通路: Metabolic Enzyme/Protease储存式: -20C, stored under nitrogen* In solvent : -80C, 6 months; -20C, 1

2、month (stored undernitrogen)溶解性数据体外实验 DMSO : 100 mg/mL (266.98 mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.6698 mL 13.3490 mL 26.6980 mL5 mM 0.5340 mL 2.6698 mL 5.3396 mL10 mM 0.2670 mL 1.3349 mL 2.6698 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当

3、的溶解案，配制前请先配制澄的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄的储备液可以根据储存条件，适当保存；以下溶剂前的百分指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (6.67 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.5 mg/mL (6.67 mM); Clear solutionBIOLOGICAL ACTIVITY

4、1/2 Master of Small Molecules 您边的抑制剂师www.MedChemE物活性 3-Oxo-5-cholanoic acid (Dehydrolithocholic acid)，胆汁酸代谢产物，通过直接与关键转录因RORt(Kd=1.13 M) 结合抑制 TH17 细胞分化。体外研究 Treatment with 3-Oxo-5-cholanoic acid significantly reduced the activity of the RORt reporter. These datasuggest that 3-Oxo-5-cholanoic acid pro

5、bably inhibits TH17 cell differentiation by physically interacting withRORt, and inhibiting its transcriptional activity 1.体内研究 3-Oxo-5-cholanoic acid (Dehydrolithocholic acid) (0.3% (w/w); p.o.; for 1 week) significantly reduces thepercentage of ileal TH17 cells 1.Animal Model: B6 Jax mice (with a

6、faecal slurry containing SFB) 1Dosage: 0.3% (w/w)Administration: Gavaged; for 1 weekResult: Significantly reduced the percentage of ileal TH17 cells.REFERENCES1. Hang S, et al. Bile acid metabolites control TH17 and Treg cell differentiation. Nature. 2019 Dec;576(7785):143-148.McePdfHeightCaution: Product has not been fully validated for medical applications.For research use only.Tel: 400