Brassinolide-Brassin-lactone-DataSheet-生命科学试剂-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEBrassinolideCat. No.: HY-N0273CAS No.: 72962-43-7Synonyms: Brassin lactone分式: CHO分量: 480.68作靶点: Others作通路: Others储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 5.2 mg/mL (10.82 mM)* means

2、soluble, but saturation unknown.Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 2.0804 mL 10.4019 mL 20.8039 mL5 mM 0.4161 mL 2.0804 mL 4.1608 mL10 mM 0.2080 mL 1.0402 mL 2.0804 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。BIOLOGICAL ACTIVITY物活性 Brassinolide种主 的植物长调节剂，可调节植物细胞伸长。IC50 & Target pla

3、nt growth modulator 1体外研究Brassinolide is a plant sterol first isolated from pollen of rape (Brassica napus L.). Brassinolide can induce a1/2 Master of Small Molecules 您边的抑制剂师www.MedChemEtime and concentration-dependent cytotoxicity in PC-3 cells. The mode of cell death appears to bepredominately apo

4、ptosis, as shown by flow-cytometric analysis, fluorescence and transmission electronmicroscopes. Caspase-3 activity is obviously increased after Brassinolide treatment. Western blot studiesindicate that treatment with Brassinolide triggered a time-dependent decrease in the expression of anti-apoptot

5、ic protein Bcl-2, which suggests that Brassinolide can induce cytotoxicity in PC-3 cells by triggeringapoptosis. Brassinolide might therefore be a promising candidate for the treatment of prostate cancer 1.Brassinolide is a plant growth modulator, on multidrug resistance (MDR) of human T lymphoblast

6、oid cell lineCCRF-VCR 1000 which is obtained by progressively addition of vincristine (VCR) to sensitive CCRF-CEMcells, and to explore preliminarily the mechanism of reversing action. After treatment of Brassinolide underthe concentration of 0.001-10 g/mL, the resistance of CCRF-VCR is reversed part

7、ly with the reversing foldsrespectively as 4.4-11.6. The intracellular accumulation of rhodamine 123 is significantly reduced in theresistant cells. After treatment of Brassinolide, the accumulation increased, the level of fluorescent dye issituated between resistant cells and sensitive cells. No al

8、teration of the catalytic activity of topoisomerase II isfound among three groups. The level of protein expression of p53 in resistant cells is higher than that ofsensitive cells. After Brassinolide treatment, the expression of p53 in CCRF-VCR cells restored to the level ofsensitive cells. Brassinol

9、ide can effectively reverse the resistance of CCRF-VCR cells by inhibiting theeffusion of drug transported by P-glucoprotein. To down regulate the abnormal expression of p53 maybe oneof the mechanisms of reversing MDR for Brassinolide 2.PROTOCOLCell Assay 2 MTT method is used to detect the resistant

10、 factor of resistant cell line and the reversing fold after addition ofBrassinolide. The intracellular accumulation of rhodamine 123, a fluorescent dye transported by P-glycoprotein is detected by flow cytometry, the catalytic activity of topoisomerase II is assessed by Sullivenmethod to find the ef

11、fect of Brassinolide on resistance. The protein expression of p53 is measured usingWestern blotting in the sensitive cells and resistant cells to explore the effect of Brassinolide 2.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Wu YD, et

12、 al. Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells.Pharmazie. 2007 May;62(5):392-5.2. Xian LJ, et al. Reversing effect of brassinolide on multidrug resistance of-CCRF-VCR1000 cells and a preliminary investigation on itsmechanisms. Yao Xue Xue Bao. 2005 Feb;40(2):117-21.McePdfHeightCaution: