Bergamottin-5-Geranoxypsoralen-DataSheet-MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEBergamottinCat. No.: HY-N2194CAS No.: 7380-40-7Synonyms: 5-Geranoxypsoralen; Bergamotine; Bergaptin分式: CHO分量: 338.4作靶点: Cytochrome P450作通路: Metabolic Enzyme/Protease储存式: 4C, protect from light* In solvent : -80C, 6 months; -20C,

2、 1 month (protect fromlight)BIOLOGICAL ACTIVITY物活性 Bergamottin种有效的竞争性 CYP1A1 抑制剂，Ki 为 10.703 nM。IC50 & Target CYP1A1 CYP1A1 CYP1A2 CYP2B210.703 nM (Ki) 0.192 M (IC50) 5.077 M (IC50) 4.535 M (IC50)CYP2B19.495 M (IC50)体外研究 Bergamottin is a competitive inhibitor of CYP1A1. Bergamottin inhibits CYP1A1,

3、CYP1A2, CYP2B1, andCYP2B2 with IC50s of 0.1920.029 M, 5.0770.31 M, 9.4950.979 M, 4.5350.092 M, respectively 1.Bergamottin has potent antiproliferative effects on the A549 cells. Bergamottin shows both concentration-dependent as well as timedependent growth inhibitory effects against these cells. Ber

4、gam ottin also inhibits theclonogenic activity of the A549 cancer cells by reducing the num ber of cancer colony form ing cells. A reduction inclonogenicity also follows the concentration dependence on Bergam ottin 2.体内研究The anticancer efficacy of Bergamottin under in vivo conditions using female BA

5、LB/c nude mice (a total of 20mice are used) is determined. Tumors are induced in the mice by injecting non-small cell lung cancer A549cells (1106 cells/mouse). After tumor formation, the mice are sacrificed and tumors are removed and theirweights and volumes are calculated. The results show that 25,

6、 50 and 100 mg/kg Bergamottin injectionreduce the tumor weight from 1.61 g in the PBS-treated group (control) to 1.21, 0.42 and 0.15 g, respectively.Tumor weight in the nude mice is reduced much more significantly in the highest-concentration Bergamottin1/3 Master of Small Molecules 您边的抑制剂师www.MedCh

7、emEgroup (100 mg/kg body weight) compared with the vehicle group (P3 in the PBS-treated group (control) to1.71, 1.1 and 0.51 cm3, respectively. The periodic measurement of the tumor xenograft volume indicates thatthe tumor volume in the nude mice is reduced considerably in the highest-concentration

8、Bergamottin group(100 mg/kg body weight) compared with the vehicle group (P 2.PROTOCOLKinase Assay 1 CYP1A1 Supersomes and different concentrations of 7-ethoxyresorufin (ER) are used to determine CYP1A1enzymatic kinetics. A typical Michaelis-Menten curve is found. The final reaction mixture contains

9、: 1 pmolCYP1A1, 0.5 mM NADPH, different ER concentrations and 0, 4, 8 and 16 nM of Bergamottin (BG). Thereaction is started with the addition of NADPH. Kinetic constants are obtained by a nonlinear regressionanalysis of experimental data fitted to Michaelis-Menten equation with competitive-type inhi

10、bition. Kineticanalysis is also shown by using the Lineweaver-Burk, Dixon and replot of the slopes of the Dixon plot 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.Cell Assay 2 Inhibition of cell proliferation by Bergamottin is measured by the MTT as

11、say. Briefly, the human lungadenocarcinoma cancer A549 cells are plated in 96-well culture plates (1105 cells/well). After 24 h ofincubation, the cells are treated with Bergamottin (0, 5, 10, 25, 50, 75 and 100 M) for 24 and 48 h, MTTsolution (10 mg/mL) is then added to each well. After a 4-h incuba

12、tion, the formazan precipitate is dissolvedin 100 L DMSO, and then the absorbance is measured in an automated microplated reader at 570 nm. TheCaution: Product has not been fully validated for medical applications.cell viability ratio is calculated. Cytotoxicity is expressed as the concentration of

13、Bergamottin needed to inhibitFor research use only.cell growth by 50% (IC50 value) 2.Tel: 400-820-3792;Fax:E-mail: techMedChemEMCE has not independently confirmed the accuracy of these methods. They are for reference only.Animal Mice 2Administration 2 Female BALB/c nude m

14、ice (six weeks old) (a total of 20 are obtained) are maintained with water and food adlibitum in a pathogen-free environment with a 12 h light and 12 h dark cycle in an animal care facility. Humannonsm all cell lung carcinom a A549 cells (2106 cells/m ouse) are injected into the right axilla of the

15、nude m ice (5m ice/group) to create tum ors in the m ice. Subsequent to tum or developm ent, the m ice are divided into 4 groups andtreated with Bergam ottin injected intraperitoneally. The control group in the study is treated with an equal am ount ofPBS while the other three groups are treated wit

16、h 25, 50 and 100 m g/kg of Bergam ottin. Afterwards, the m ice aresacrificed after 18 days, and the tum or weight and volum e of each m ouse are evaluated. Tum or length and width are m easured using a Vernier caliper and the tum or volum e (TV) is calculated 2.MCE has not independently confirmed th

17、e accuracy of these methods. They are for reference only.REFERENCES1. Olgun-Reyes S, et al. Bergamottin is a competitive inhibitor of CYP1A1 and is antimutagenic in the Ames test. Food Chem Toxicol.2012 Sep;50(9):3094-9.2. Wu HJ, et al. Bergamottin isolated from Citrus bergamia exerts in vitro and in