二十二碳六烯酸作用机制 - Medchemexpress - MCE中国

1、Product Data SheetDocosahexaenoic AcidCat. No.: HY-B2167CAS No.: 6217-54-5分式: CHO分量: 328.49作靶点: Endogenous Metabolite作通路: Metabolic Enzyme/Protease储存式: 4C, stored under nitrogen* In solvent : -80C, 6 months; -20C, 1 month (stored under nitrogen)溶解性数据体外实验 Ethanol : 50 mg/mL (152.21 mM; Need ultrasoni

2、c)DMSO : 5.2 mg/mL (15.83 mM; Need ultrasonic)H2O : 0.1 mg/mL (insoluble)SolventMass1 mg 5 mg 10 mgConcentration制备储备液1 mM 3.0442 mL 15.2212 mL 30.4423 mL5 mM 0.6088 mL 3.0442 mL 6.0885 mL10 mM 0.3044 mL 1.5221 mL 3.0442 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 month

3、s; -20C, 1 month (stored under nitrogen)。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使。体内实验请根据您的实验动物和给药式选择适当的溶解案。以下溶解案都请先按照 In Vitro 式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天使； 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 4

4、5% salineSolubility: 0.52 mg/mL (1.58 mM); Clear solution此案可获得 0.52 mg/mL (1.58 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100 L 5.2 mg/mL 的澄 DMSO 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加50 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 0.52 mg/mL (1.58 mM); Clear solution此案可获得 0.5

5、2 mg/mL (1.58 mM，饱和度未知) 的澄 溶液，此案不适于实验周 期在半个以上的实验。Page 1 of 2 www.MedChemE以 1 mL 作液为例，取 100 L 5.2 mg/mL 的澄 DMSO 储备液加到 900 L 油中，混合均匀。3. 请依序添加每种溶剂： 10% EtOH 40% PEG300 5% Tween-80 45% salineSolubility: 5 mg/mL (15.22 mM); Clear solution此案可获得 5 mg/mL (15.22 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例，取 100 L 50.0 mg/mL

6、 的澄 EtOH 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加50 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。4. 请依序添加每种溶剂： 10% EtOH 90% (20% SBE-CD in saline)Solubility: 5 mg/mL (15.22 mM); Suspended solution; Need ultrasonic此案可获得 5 mg/mL (15.22 mM) 的均匀悬浊液，悬浊液可于服和腹腔注射。以 1 mL 作液为例，取 100 L 50.0 mg/mL 的澄 EtOH 储备液加到 900 L 20% 的

7、SBE-CD 理盐溶液中，混合均匀。5. 请依序添加每种溶剂： 10% EtOH 90% corn oilSolubility: 5 mg/mL (15.22 mM); Clear solution此案可获得 5 mg/mL (15.22 mM，饱和度未知) 的澄 溶液，此案不适于实验周 期在半个以上的实验。以 1 mL 作液为例，取 100 L 50.0 mg/mL 的澄 EtOH 储备液加到 900 L 油中，混合均匀。BIOLOGICAL ACTIVITY物活性 Docosahexaenoic Acid (DHA)丰富地存在于脑和视膜中的-3脂肪酸，可以直接从鱼油和母乳中获得。IC &

8、Target Human Endogenous Metabolite体外研究 Docosahexaenoic acid (DHA) is essential for the growth and functional development of the brain in infants. DHA isalso required for maintenance of normal brain function in adults. The inclusion of plentiful DHA in the diet improveslearning ability and memory1. D

9、HA is an essential requirement in every step of brain development like neural cellproliferation, migration, differentiation, synaptogenesis. The multiple double bonds and unique structure allow DHAto impart special membrane characteristics for effective cell signaling. Many development disorders lik

10、e dyslexia,autism spectrum disorder, attention deficit hyperactivity disorder, schizophrenia etc. are causally related to decreasedlevel of DHA2. DHA is a potent RXR ligand inducing robust RXR activation already at low micro molar concentrations.The EC50 for RXR activation by DHA is about 5-10 M fat

11、ty acid3.体内研究 Docosahexaenoic acid administration over 10 weeks significantly reduces the number of reference memory errors,without affecting the number of working memory errors, and significantly increases the docosahexaenoic acidcontent and the docosahexaenoic acid/arachidonic acid ratio in both t

12、he hippocampus and the cerebral cortex4.DHA treatment exerts neuroprotective actions on an experimental mouse model of PD. There is a decrease tendencyin brain lipid oxidation of MPTP mice but it does not significantly5.PROTOCOLAnimal Rats: Wistar rats are fed a fish oil-deficient diet through three

13、 generations. The young (five-week-old) male rats of theAdministration 45 third generation are randomLy divided into two groups. Over 10 weeks, one group is perorally administereddocosahexaenoic acid dissolved in 5% gum Arabic solution at 300 mg/kg/day; the other group receives a similarvolume of ve

14、hicle alone. Five weeks after starting the administration, the rats are tested for learning ability related totwo types of memory, reference memory and working memory, with the partially (four of eight) baited eight-armradial maze4.Page 2 of 3 www.MedChemEMice: An experimental model of Parkinsons di

15、sease is created by four intraperitoneal injections of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (420 mg/kg, at 12 h intervals). Docosahexaenoic acid is given daily bygavage for 4 weeks (36 mg/kg/day). The motor activity of the mice is evaluated via the pole test. The activity ofantioxidan

16、t enzymes in the brain are determined by spectrophotometric assays and the concentration ofthiobarbituric acid-reactive substances (TBARS) are measured as an index of oxidative damage5.MCE has not independently confirmed the accuracy of these methods. They are for reference only.户使本产品发表的科研献 Fish She

17、llfish Immunol. 2019 Sep 6. pii: S1050-4648(19)30889-7. Pathol Res Pract. 2019 Dec.See more customer validations on HYPERLINK www.MedChemE www.MedChemEREFERENCES1. Horrocks LA, et al. Health benefits of docosahexaenoic acid (DHA). Pharmacol Res. 1999 Sep;40(3):211-25.2. Gharami K, et al. Essential r

18、ole of docosahexaenoic acid towards development of a smarter brain. Neurochem Int. 2015 Oct;89:51-62.3. Lengqvist J, et al. Polyunsaturated fatty acids including docosahexaenoic and arachidonic acid bind to the retinoid Xreceptor alpha ligand-bindingdomain. Mol Cell Proteomics. 2004 Jul;3(7):692-703.4. Gamoh S, et al. Chronic administration of docosahexaenoic acid improves reference memory-related learning ability in young rats. Neuroscience.1999;93(1):237-41.5. Ozsoy O, et al. The influence and the mechanism of docosahexaenoic acid on a mouse m