各种缓冲液的配制方法大全

1、磷酸氢二，钠柠檬酸缓冲液0.2mol/L0.1mol/L0.2mol/L0.1mol/LPHNa2HPO4柠檬酸pHNa2HPO4柠檬酸（毫升）（毫升）（毫升）（毫升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.55

2、4.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4 分子量=14.98, 0.2mol/L 溶液为 28.40 克/升。Na2HPO4-2H2O 分子量=178.05, 0.2mol/L 溶液含 35.01 克/升。C4H2O7-H2O 分子量=210.14, 0.1mol/L 溶液为 21.01 克/升20%盐酸溶液如何配置取浓盐酸（质量百分比

3、浓度为36.5%） 一个体积（如100毫升），加入到96.5毫 升水 中就可 以了。A36.5%*100*1.17=20%*mAm=213.5（克）A所需水 的质量 为;213.5-117=96.5克，也就是96.5毫升水。磷酸盐缓冲液配制方法（PB）磷酸盐缓冲液（Phosphate Buffer, PB）试剂：NaH2PO42H2ONa2HPO/12H2O配制方法：配制时，常先配制0.2mol/L的NaH2PO4和0.2mol/L的Na2HPO4，两者按一定比例 混合即成0.2mol/L的磷酸盐缓冲液（PB），根据需要可配制不同浓度的PB和PBS。（1）0.2mol/L 的 NaH2PO4；

4、称取 NaH2PO4.2H2O 31.2g（或 NaH2PO4-H2O 27.6g）加重蒸 水至1000ml溶解。（2）0.2mol/L 的 Na2HpO4：称取 Na2HpO4.12H2O 71.632g（或 Na2HpO/HR 53.6g 或 Na2HPO4-2H2O 35.6g）加重蒸水至 1000ml 溶解。（3）0.2mol/L pH7.4 的 PB 的配制：取 19ml 0.2mol/L 的 NaH2P。4和 81ml 0.2mol/L 的 Na2HpO4-12H2O,充分混合即为0.2mol/L的PB （pH约为7.47.5）。若pH偏高或偏低，可通过改 变二者的比例来加以调整，

5、室温保存即可。0.2mol/L磷酸盐缓冲液（pH5.78.0）pH0.2mol/L NaH2PO4(ml)0.2mol/L Na2HpO4(ml)5.793.56.55.892.08.05.990.010.06.087.712.36.185.015.06.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.56.851.049.06.945.055.07.039.061.07.133.067.07.228.072.07.323.067.07.419.081.07.516.084.07.613.087.07.710.589

6、.57.88.591.57.97.093.08.05.394.70.05mol/L磷酸盐缓冲液少K.0）如何配制？PH0.05mol/L NaH2PO4(ml) 0.05mol/L Na2HPO4(ml)5.793.56.55.892.08.05.990,010.06.087.712.36.185,015.06.281.518.56.377.522.56.473.526.56,568.531.56.662.537.56.756,543.56.851.049.06.94505507.039.061.07.133.067.07.228.072.07.323.067.07.419.081.07.51

7、6.084.07.613.087.07.710.589.57.88.591.57.97.093.08.,05.394.72 5K下0.1m 口 l/L磷酸钾缓冲液的配制pH0.05mol/L K2HPO4(ml)0.05mol/L KHI2PO4(ml)5.88.591.56.013.286.86.219.280.86.427.872.26.638.161.96.849.750.37.061.538.57.271.728.37.480.219.87.686.613.47.890.89.28.0946.0PH3-10缓冲溶液的配制方法1.磷酸氢二钠-柠檬酸缓冲液pH0.2mol/LNa2HPO4

8、(毫升)0.1mol/L柠檬酸 （毫升）PH0.2mol/LNa2HpO4(毫升)0.1mol/L柠檬酸 （毫升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.87.1012.906.815.454.554.07.7112.297.016.473.534.2

9、8.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HpO4分子量=14.98, 0.2 mol/L 溶液为 28.40 克/升。Na2HpO4 2H2O 分子量=178.05, 0.2 mol/L 溶液含 35.01 克/升。C4H2O7 H2O 分子量=210.14, 0.1 mol/L 溶液为 21.01 克/升。2.柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L）柠檬酸（克）C6H8O7 *

10、凡0氢氧化钠（克）NaOH 97%盐酸（毫升）HCl （浓）最终体积 （升）2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610 使用时可以每升中加入1克克酚，若最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。3.甘氨酸-盐酸缓冲液（0.05mol/L）X毫升0.2 mol/L甘氨酸+Y毫升0.2 mol/L HCI,再加水稀释至200毫升PHXYPHXY2.05044.03.05011.

11、42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量=75.07, 0.2 mol/L甘氨酸溶液含15.01克/升。4.邻苯二甲酸-盐酸缓冲液（0.05 mol/L）X毫升0.2 mol/L邻苯二甲酸氢钾+ 0.2 mol/L HCl,再加水稀释到20毫升pH(20)XYpH(20)XY2.254.0703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾分子量=204.23, 0.2 mol/L邻苯二甲酸氢溶液含40.85克

12、/升5.柠檬酸-柠檬酸钠缓冲液（0.1 mol/L）pH0.1 mol/L柠檬酸（毫升）0.1 mol/L柠檬酸钠（毫升）pH0.1 mol/L柠檬酸 （毫升）0.1 mol/L柠檬酸钠（毫升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39.76.61.418.64.89.210.8柠檬酸C6H8O7 H2O

13、：分子量210.14, 0.1 mol/L溶液为21.01克/升。柠檬酸钠 Na3 C6H5O7 - 2H2O：分子量 294.12, 0.1 mol/L 溶液为 29.41 克/升。柠檬酸 C6H8O7 - H2O: 2.1g柠檬酸钠 Na3 C6H5O7 - 2H2O: 2.94gH2O: 100 ml6.乙酸-乙酸钠缓冲液(0.2 mol/L)pH（18）0.2 mol/LNaAc （毫升）0.3 mol/LHAc（毫升）pH（18）0.2 mol/LNaAc （毫升）0.3 mol/LHAc（毫升）2.60.759.254.85.904.103.81.208.805.07.003.00

14、4.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60Na2Ac-3H2O 分子量=136.09, 0.2 mol/L 溶液为 27.22 克/升。7.磷酸盐缓冲液(1)磷酸氢二钠-磷酸二氢钠缓冲液(0.2)PH0.2 mol/LNa2HpO4（毫升）0.2 mol/LNaH2PO4（毫升）PH0.2 mol/LNa2HpO4（毫升）0.2 mol/LNaH2PO4 （毫升）5.88.092.07.061.039.05.910.090.07.167.033.06.012.3

15、87.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.955.045.0Na2HpO4-2H2O 分子量=178.05, 0.2 mol/L 溶液为 85.61 克/升。Na2HpO4- 12H2O 分子量=358.22, 0.2 mol/L 溶液为 71.64 克/升。N

16、a2HpO4-2H2O 分子量=156.03, 0.2 mol/L 溶液为 31.21 克/升。（2）磷酸氢二钠-磷酸二氢钾缓冲液（1/15 mol/L）pHmol/L/15NaHPOJ24（毫升）mol/L15KHePO,24（毫升）pHmol/L/15NaHPO,24（毫升）mol/L15KHePO,24（毫升）4.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.242.008.008.049.500.506.473.007.008.349.750.256.644.006.008.679.900

17、.106.815.005.008.1810.0006.986.004.00Na2HpO4-2H2O 分子量=178.05, 1/15M 溶液为 11.876 克/升。KH2PO4分子量=136.09, 1/15M 溶液为 9.078 克/升。8.磷酸二氢钾-氢氧化钠缓冲液（0.05M）X毫升0.2M K2PO4 + Y毫升0.2N NaOH加水稀释至29毫升pH（20）X （毫升）Y （毫升）pH（20）X （毫升）Y （毫升）5.850.3727.052.9636.050.5707.253.5006.250.8607.453.9506.451.2607.654.2806.651.7807.8

18、54.5206.852.3658.054.6809.巴比妥钠-盐酸缓冲液（18）Ph0.04M巴比妥钠溶液0.2V盐酸PH0.04M巴比 妥钠溶液（毫 升）0.2N盐酸（毫 升）6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10. Tris-盐酸缓冲液（0.05M, 25）5

19、0毫升0.1M三羟甲基氨基甲烷（Tris）溶液与X毫升0.1N盐酸混匀后，加水稀释至100 毫升。pH乂（毫升）pHX（毫升）7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2三羟甲基氨基甲烷（Tris） HOCH2 CH2OHCHOCH2 NH2分子量=121.14;0.1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳，使用时注意

20、将瓶盖严。11.硼酸-硼砂缓冲液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼砂（毫升）pH0.05M硼砂（毫升）0.2M硼酸（毫升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂Na2B4O7-H2O,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BO3,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。11.硼酸-硼砂缓冲液（0.2M硼酸根）pH0.05M硼砂（毫升）0.2M硼酸 （毫升）pH0.05M硼砂（毫升）0.2M硼酸 （毫升）7.41.09.08.23.56.57.61.58.58.44.55.57.82.08.08.76.04.08.03.07.09.08.02.0硼砂 Na2B4O7- 10H2O,分子量=381.43;0.05M 溶液（=0.2M 硼酸根）含 19.07 克/升。硼酸H2BO3，分子量=61.84, 0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的