各种激发光和吸收光波长

1、各种激发光和吸收光波长现在发现一个更全的有关激发光和发射光的波长图谱。这对CONFOCA法做多重染色时是必不可少的参考资料。全部文件请到这里下载！ExcitationandEmissionWavelengthsofFluorophoresTavi's"FluoroTable":Commonfluorophores(fromZeissCorporationwebsite)FluorophoreAbsorptionEmissionOtherinfo1,5IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carb

2、oxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein(5-FAM)4925185-Carboxynapthofluorescein(pH10)512/598563/668RatioDye,pH5-Carboxytetramethylrhodamine542568(5-TAMRA)5-FAM(5-Carboxyfluorescein)4925185-HAT(HydroxyTryptamine)370-415520-5405-HydroxyTryptamine(HAT)370-415520-5405-ROX(carboxy-X-rhodamin

3、e)5786045675915-TAMRA548552(5-Carboxytetramethylrhodamine)5425686-Carboxyrhodamine6G5185436-CR6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-AminoactinomycinD(7-AAD)5466477-Hydroxy-4-methylcoumarin360449,4559-Amino-6-chloro-2-methoxyacridine412,430471,474ABQ344445AcidFuchsin540630ACMA412,430471,

4、474(9-Amino-6-chloro-2-methoxyacridine)AcridineOrange+DNA502526AcridineOrange+RNA460650AcridineOrange,bothDNA&RNA440-480520-650AcridineRed455-600560-680AcridineYellow470550Acriflavin436520AcriflavinFeulgenSITSA355-425460Aequorin(Photoprotein)466PhotoproteinAFPs-AutoFluorescentProtein-(QuantumBio

5、technologies)seesgGFPsgBFPAlexaFluor350346442342441AlexaFluor430431540AlexaFluor488495,492519,520AlexaFluor532531,532553,554AlexaFluor546556,557572,573AlexaFluor568577,578603AlexaFluor594590,594617,618AlexaFluor633632650AlexaFluor647647666AlexaFluor660668698AlexaFluor680679702AlizarinComplexon530-56

6、0,580,580624-645AlizarinRed530-560580Allophycocyanin(APC)630,645655,660AMC,AMCA-S345445AMCA(Aminomethylcoumarin)345425347444AMCA-X353442AminoactinomycinD555655Aminocoumarin346442350445Aminomethylcoumarin(AMCA)345425347444AnilinBlue600Anthrocylstearate360-381446APC(Allophycocyanin)630,645655,660APC-C

7、y7625-650755APTRA-BTC=RatioDye,Zn2+466/380520/530RatioDye,Zn2+APTS424505AstrazonBrilliantRed4G500585AstrazonOrangeR470540AstrazonRed6B520595AstrazonYellow7GLL450480Atabrine436490ATTO-TAG?CBQCA465560ATTO-TAG?FQ486591Auramine460550AurophosphineG450580Aurophosphine450-490515BAO9(Bisaminophenyloxadiazol

8、e)365395BCECF(highpH)492,503520,528BCECF(lowpH)482520BerberineSulphate430550BetaLactamase409447,520BFPblueshiftedGFP(Y66H)381,382,445,447,blueshiftedGFP(Y66H)BlueFluorescentProteinBFP/GFPFRET383448BlueFluorescentProteinBimane398490Bisbenzamide360461Bisbenzimide(Hoechst)360461bis-BTC=RatioDye,Zn2+455

9、/405529/505RatioDye,Zn2+BlancophorFFG390470BlancophorSV370435BOBO?-1462481BOBO?-3570602Bodipy492/515490515Bodipy493/503533549Bodipy500/510509515Bodipy505/515502510Bodipy530/550528547Bodipy542/563543563Bodipy558/568558569Bodipy564/570564570Bodipy576/589579590Bodipy581/591584592Bodipy630/650-X625642Bo

10、dipy650/665-X647665Bodipy665/676605676BodipyFl504,505511,513BodipyFLATP505514BodipyFl-Ceramide505511BodipyR6GSE528547BodipyTMR542574BodipyTMR-Xconjugate544573BodipyTMR-X,SE544570BodipyTR589617BodipyTRATP591620BodipyTR-XSE588616BO-PRO?-1462481BO-PRO?-3544570BrilliantSulphoflavinFF430520BTC-RatioDyeCa

11、2+464/401533/529RatioDyeCa2+BTC-5N-atioDye,Zn2+459/417517/532RatioDye,Zn2+Calcein494517CalceinBlue373440CalciumCrimson588,589611,615CalciumGreen501,506531CalciumGreen-1Ca2+Dye506531Ca2+DyeCalciumGreen-2Ca2+506/503536Ca2+CalciumGreen-5NCa2+506532Ca2+CalciumGreen-C18Ca2+509530Ca2+CalciumOrange54957557

12、6CalcofluorWhite385,395,437,440,405445Carboxy-X-rhodamine(5-ROX)576601CascadeBlue377420398423399CascadeYellow399550400552Catecholamine410470CCF2(GeneBlazer)CFDA494520CFP-CyanFluorescentProtein430,433,474,475,CyanFluorescentProtein436,(453)476,(501)CFP/YFPFRETChlorophyll480650ChromomycinA436-460470Ch

13、romomycinA445575CL-NERF(RatioDye,pH)504/514540RatioDye,pHCMFDA494520CoelenterazineCa2+Dye,bioluminescence(429)465Ca2+Dye,bioluminescence,nativemoleculeCoelenterazinecp(Ca2+Dye,)(430)442Ca2+Dye,bioluminescenceCoelenterazinef(437)473Ca2+Dye,bioluminescenceCoelenterazinefcp452Ca2+Dye,bioluminescenceCoe

14、lenterazineh(437)464Ca2+Dye,bioluminescenceCoelenterazinehcp(433)444Ca2+Dye,bioluminescenceCoelenterazineip441Ca2+Dye,bioluminescenceCoelenterazinen(431)467Ca2+Dye,bioluminescenceCoelenterazineO460575CoumarinPhalloidin387470C-phycocyanineCPMMethylcoumarin384469MethylcoumarinCTC400-450602CTCFormazanC

15、y2489506Cy3.18554568Cy3.5581598Cy3514566552570554Cy5.18649666Cy5.5675695Cy5649666670Cy7710,743767,805CyanGFP433(453)475(501)cyclicAMPFluorosensor(FiCRhR)500517CyQuantCellProliferationAssay480520CellProliferationAssayDabcyl453Dansyl340578DansylAmine337517DansylCadaverine335518DansylChloride372518Dans

16、ylDHPE336517Dansylfluoride356noneDAPI359461Dapoxyl403580Dapoxyl2374574Dapoxyl3373574DCFDA504529DCFH(Dichlorodihydrofluorescein505535Diacetate)DDAO463607DHR(Dihydorhodamine123)505534Di-4-ANEPPS496705Di-8-ANEPPS(non-ratio)488605498713DiA(4-Di-16-ASP)456591DichlorodihydrofluoresceinDiacetate505535(DCFH

17、)DiD-LipophilicTracer644665LipophilicTracerDiD(DiIC18(5)644665DIDS341415Dihydorhodamine123(DHR)505535DiI(DiIC18(3)549,551565Dinitrophenol349DiO(DiOC18(3)484,487501,502DiR748780LipophilicTracerDiR(DiIC18)750779DM-NERF(highpH)497/510540RatioDye,pHDNP349Dopamine340490-520DsRed558583Redfluorescentprotei

18、nDTAF494520DY-630-NHS621660HemicyanelabelforproteinsandDNADY-635-NHS634664HemicyanelabelforproteinsandDNAEBFP383447EnhancedBlueFluorescentProteinECFP436474EnhancedCyanFluorescentProteinEGFP488,498507,516EnhancedGreenFluorescentProteinELF97345530Eosin524545Erythrosin529,532554,555ErythrosinITC529555E

19、thidiumBromide510,523595,605Ethidiumhomodimer-1(EthD-1)528617EuchrysinEukoLightEuropium(III)chloride430540EYFP513,520527,532EnhancedYellowFluorescentProteinFastBlue360440FDA494520Feulgen(Pararosaniline)570625FIF(FormaldehydInducedFluorescence)405433FITC490,494520,525FITCAntibody493517FlazoOrange375-

20、530612Fluo-3480?506,520,527506Fluo-4494516Fluorescein(FITC)490,494520,525FluoresceinDiacetate494520Fluoro-Emerald495524Fluoro-Gold(Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM1-43479598FM4-46515640FuraRed?(highpH)FuraRed?Fluo-3572657Fura-2,highcalcium335505ExcitationratiodyeFura-2,lowcal

21、cium363512ExcitationratiodyeFura-2/BCECFGenacrylBrilliantRedB520590GenacrylBrilliantYellow10GF430485GenacrylPink3G470583GenacrylYellow5GF430475GeneBlazer(CCF2)GFP(S65T)498516GFPredshifted(rsGFP)498516GFPwildtype,non-UVexcitation(wtGFP)475509GFPwildtype,UVexcitation(wtGFP)395509GFPuv385508GloxalicAcid405460GranularBlue355425Haematoporphyrin530-560580Hoechst3325834