新城疫抗体血凝抑制试验比对结果分析.doc

1、新城疫抗体血凝抑制试验比对结果分析p 现代农业科技20_年第22期动物科学【摘要】：p 为掌握某兽医实验室新城疫抗体血凝抑制(HI )试验能力,用新城疫标准阳性血清和阴性血清制备52份盲样,由6名检测技术员同时进行HI 抗体检测。将检测结果与参考值进行比较,计算阳性、阴性一致性以及符合率,并用 Episcope 2.0软件进行Ka 一致性检验,计算二者差值并进行统计描述。结果表明,312个检测值阳性率为13.5,低于参考值15.4的阳性率(p 【关键词】：p 新城疫抗体;血凝抑制试验;比对试验;Ka 检验中图分类号S852.4+3文献标识码A 文章编号1007-5739(20_)22-0230

2、-02parison of HI Method for Detection of Newcastle Disease Antibody LIU Hua CHEN _i ZHU Liang-qiang ZHAN Song-he HE Chang-sheng(Anhui Provincial Center for Animal Disease Control and Prevention ,Hefei Anhui 230061)Abstract A parison of HI method for detection of Newcastle Disease (ND )antibody was c

3、onducted to estimate the test capability of HI method at a veterinary laboratory.The standard NDV antibody positive serum which was diluted into different titers ,together with the standard NDV-antibody-negative ones were prepared totally 52for the 6technicians who did the HI test at the same time.T

4、he HI titers of the 52sles were observed by the staffs and were pared with the reference values.The positive and negative coincidence ,the coincidence rate were calculated separately and Ka -test was conducted by using Episcope 2.0software.Meanwhile ,difference between the tested and the reference v

5、alues was described by statistics method.The results showed that positive rate was 13.5,which showed a statistic difference with the reference 15.4(p Key words Newcastle Disease antibody ;hemagglutination inhibition test ;parison test ;Ka-test新城疫抗体血凝抑制试验比对结果分析p 刘华陈曦朱良强占松鹤何长生(安徽省动物疫病预防与控制中心,安徽合肥23006

6、1)为适应当时畜牧业生产发展的需要,某兽医实验室成立于19_年。近些年,受财政预算减少等因素影响,政府采取兽医实验室商业化、私有化等措施,如为养殖户和利益相关方提供有偿兽医实验室检测服务,以解决当前的问题。但兽医实验室也面临着科研人才流失、仪器设备老化和日益复杂的动物疫病流行形势等诸多挑战。为掌握该兽医实验室血凝抑制试验(HI )的检测能力,由6名具备检测资质的技术人员同时进行HI 抗体检测,笔者对试验结果进行了比对分析p 。1材料与方法1.1试验材料新城疫标准阳性血清(批号:2/12),新城疫标准阴性血清(批号:06/15A ),新城疫标准检测抗原(批号:080920_),均由意大利IZSA

7、M 实验室提供。1.2试验方法将鸡新城疫标准阴性、阳性血清分别进行倍比稀释后分装,共制成52份盲样,其中8份为阳性,即HI 效价116,结果(即参考值)记为N log2。6名检测技术员参照OIE 陆生动物诊断试验与疫苗手册20_第2.3.14章,对这52份盲样进行鸡新城疫病毒HI 抗体检测,检测结果记为n log2。1.3统计方法检测值(n )和参考值(N )均按4为阳性,反之为阴性进行定性判断。应用Epi Info 7对结果进行2×2表格统计(图1),计算阳性一致性(a a+c )、阴性一致性(d b+d ),用Episcope 2.0计算符合率(Observed proporti

8、on of agreement )(a+d a+b+c+d),再用 Episcope 2.0软件进行试验一致性(Agreement )分析p ,计算Ka 值,以比较HI 定性试验的符合情况。参照经典文献进行一致性强度判断,即Ka 值 0.80,高度;0.811.00,极强1。用SPSS 20和E_cel 软件对检测值和参考值的差值进行统计描述和频数分析p ,统计差值的极大值、极小值、均值、标准差、方差、频数和直方图,分析p 定量结果的总体分布情况。2结果与分析p 2.1定性结果一致性将6名检测技术员的312个检测值与参考值的定性结果全部进行相关统计,结果检测阳性42份,阳性率13.5,低于参考值15.4的阳性率(字2=148.85,P 结果的一致性为68.