pET-30a大肠杆菌表达载体-质粒-菌株

1、pET-30a大肠杆菌表达载体,pET-30a质粒,菌株编码名称规格保存条件北京华越洋生物GHT-20MGpET-30a20mg-20 C,避光,12个月北京华越洋生物GHT-100mgpET-30a100mg-20 C,避光,12个 月pET-30a大肠杆菌表达载体,pET-30a质粒,菌株 载体信息： 启动子：T7/lac复制子：pBR322 oripET-30a大肠杆菌表达载体,pET-30a质粒,菌株 载体别名：pET-30a,pET30a,pET 30a 载体分类：大肠杆菌表达载体 载体大小：5422 bp 载体抗性：卡那霉素Kan载体标签：N-His, N-S, N-Thrombi

2、n, N-EK, C-His筛选标记：克隆菌株：DH&表达宿主：BL21 (DE3)培养条件：37 C ,LB5' 测序弓I物：T7: TAATACGACTCACTATAGGG3' 测序引物:T7-TeTGCTAGTTATTGCTCAGCGG 备注：pET-30a大肠杆菌表达载体,pET-30a质粒,菌株载体简介：The maps for pET-30b(+) and pET-30c(+) are the same as pET-30a(+) (shown) with the following exceptions: pET-30b(+) is a 5421bp pl

3、asmid; subtract 1bp from each site beyond BamH I at 198. pET-30c(+) is a 5423bp plasmid; add 1bp to each site beyond BamH I at 198. The pET-30a-c(+)vectors carry an N-terminal His?Tag?/thrombin/S?Tag?/enterokinase configuration plus an optional C-terminal His?Tag sequence. Unique sites are shown on

4、the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.5H4*44K1pET30a+>(5422t)p>、中部1BWLU11 tnnj >Sm 斜iim /Eimikf*"aNW h*MiiMH,E 知GE EeoHmHEnH 叫 Z3 31 卬3件Nd«a, g smBcHlWi2 htHrnBbH膈,1打Ht&i kfflCh+ It a" ItWAHll壬3广浮-$?£温rMil HfAlfagi .SfcL 酬 *嘛 JfcloH-SJa-citonnc enrESJi on reoin操作说明:i.请使用华越洋生物定制开箱器翻开外包装；2 .