限制性内切酶保护碱基表_共5页

1、pcr设计引物时酶切位点的爱护碱基表酶寡核苷酸序列切割率 %2 hr20 hrggtcgac c00acc icggtcgac cg00ccggtcgac cgg00cacatgt g00afl iiiccacatgt gg>90>90ccc acatgt ggg>90>90ggcgcgcc>90>90asc iaggcgcgcc t>90>90tt ggcgcgcc aa>90>90ccccggg g50>90ava icccccggg gg>90>90tcccccggg gga>90>90cggatc

2、c g1025bamh icgggatcc cg>90>90cgcggatcc gcg>90>90cagatct g00bgl iigaagatct tc75>90gga agatct tcc25>90ggcgcgc c00bssh iiag gcgcgc ct00ttg gcgcgc caa50>90bste iigggta/taccc010aactgcagaaccaatgcattgg00bstx iaaaactgcagccaatgcattggaa2550ctgcagaaccaatgcattggatgcat25>90catcgatg00gatc

3、gatc00ccatcgatgg>90>90ccc atcgatggg5050ggaattcc>90>90ecor icggaattccg>90>90ccg gaattccgg>90>90cla ihae iiigg ggcc cc agc ggcc gct ttgc ggcc gcaa>90>90>90>90>90>90hind iiicaagctt g ccaagctt gg ccc aagctt ggg00001075kpn imlu igggtacc c ggggtacc cc cggggtacc cc

4、ggacgcgt c cgacgcgt cg0>90>900250>90>90050nco icccatgg g catg ccatgg catg005075ccatatgg00cccatatggg00cgc catatggcg00gggttt catatgaaaccc00ggaattc catatggaattcc75>90gggaattc catatggaattccc75>90ggctagc c00nhe icggctagc cg1025cta gctagc tag1050tt gcggccgc aa00attt gcggccgc ttta1010aaat

5、at gcggccgc tataaa1010nde inot insi iataagaatgcggccgc taaactat aaggaaaaaagcggccgc aaaaggaaaatgcatgcat gcacca atgcat tggttctgcagtt252510>9090>90>90>90pac ittaattaagttaattaac ccttaattaagg000250>90pme igtttaaac ggtttaaaccgggtttaaacccagcttt gtttaaac ggcgcgccgg0002505075>90gctgcag c00tg

6、ca ctgcag tgca1010pst iaa ctgcag aaccaatgcattgg>90>90aaaa ctgcag ccaatgcattggaa>90>90ctgcag aaccaatgcattggatgcat00ccgatcg g00pvu iat cgatcg at1025tcg cgatcg cga0101010sac icgagctc ggccgcgg c00sac ii50tccccgcgg gga>90gtcgac gtcaaaaggccatagcggccg00c1050sal igcgtcgac gtcttggccatagcggccgc

7、1075ggacgc gtcgac gtcggccatagcggccg cggaagagtact c1025sca i75aaa agtact ttt75cccggg010ccccggg g010cccccggg gg1050tcccccggg gga>90>90gactagtc10>90ggactagtcc10>90cgg actagtccg050ctag actagtctag050ggcatgcc00sph icat gcatgc atg025acat gcatgc atgt1050a aggcct t>90>90stu iga aggcct tc>

8、;90>90aaa aggcct ttt>90>90ctctaga g00xba igctctaga gc>90>90tgctctaga gca75>90sma ispe ictag tctaga ctag75>90xho icctcgag g ccctcgag gg ccgctcgag cgg0010251075ccccggg g00xma icccccggg gg2575ccc cccggg ggg50>90tccc cccggg ggga>90>90apai（类型： type ii restriction enzyme）识别序列

9、：5'gggccc3'bamhi（类型： type ii restriction enzyme）识别序列：5' ggatcc3'bglii（类型： type ii restriction enzyme）识别序列：5' agatct3'ecori（类型： type ii restriction enzyme）识别序列：5' gaattc3'hindiii（类型： type ii restriction enzyme）识别序列：5' aagctt3'kpni（类型： type ii restriction enzyme

10、）识别序列：5' ggtacc3'ncoi（类型： type ii restriction enzyme）识别序列：5' ccatgg3'ndei（类型： type ii restriction enzyme）识别序列：5' catatg3'nhei（类型： type ii restriction enzyme）识别序列：5' gctagc3'noti（类型： type ii restriction enzyme）识别序列：5'gcggccgc 3'saci（类型： type ii restriction enzyme）识别序列：5' gagctc3'3'sali（类型： type ii restriction enzyme）识别序列：5' gtcgacsphi（类型： type ii