硕士生物仪器分析之色谱概论1

1、想想看，如何 将混色m they differ in the nature of the packing. Size-Exclusion Column Adsorption Column Ion-Exchange Column separating large biomolecules like proteins or carbohydrates particle size adsorbent stationary phase level of polarity of the sample particles adsorb and de-adsorb process the strength

2、of the charge of the particles separating ionizable samples 使用领域使用领域 分析用色谱仪分析用色谱仪 半制备色谱仪或制备色谱仪半制备色谱仪或制备色谱仪 流程色谱仪流程色谱仪 (模拟移动床色谱模拟移动床色谱) 20 世纪60 年代发展起来的一种现代化分离技术 利用自动控制程序完成分离，特别是针对手性物质的分离 在制备色谱技术中最适用于进行连续性大规模工业化生产 得率：60-99%（根据不同的物质）；纯度：不低于95%； 柱数量：2-12支；日处理量：30-140000L Semi-preparative Chromatography

3、or Preparative Chromatography Analytical Chromatography Stimulated Moving Bed (SMB) 微克级(ug scale) - 毫克级(mg scale) 毫克级(mg scale) - 克级(gram scale) Publisher: Wiley-Interscience Publish year: 1998 ISBN-10: 0471172618 ISBN-13: 978-0471172611 1. 工具书或专著工具书或专著 Publisher: Elsevier Science Pages: 608 Publish

4、 year: 1993 ISBN-10: 0444815902 ISBN-13: 9780444815903 国外的主要的色谱期刊国外的主要的色谱期刊 Impact Factor (IF) Journal of Chromatography A3.756 Journal of Chromatography B2.500 Journal of Liquid Chromatography1.026 Analytical Chemistry5.712 Analytical Science1.735 Analytical Chimica Acta3.146 J. High Res. Chromatog

5、r. they differ in the nature of the packing. Size-Exclusion Column Adsorption Column Ion-Exchange Column separating large biomolecules like proteins or carbohydrates particle size adsorbent stationary phase level of polarity of the sample particles adsorb and de-adsorb process the strength of the charge o