ProtamineSulfate(最新整理)

1、protamine sulfate: detecting heparin in plasmawhen the tt is 28 seconds, it is possible that the prolonged result is due to the presence of heparin; the concentration of heparin may be enough, if the tt is 100 seconds, to also impact the result of other routine coagulation tests, in particular the a

2、ptt. since methods for removal of heparin from plasma can be time-consuming and somewhat expensive, it is possible to use protamine sulfate to demonstrate whether or not heparin is likely the cause of the prolonged tt. this is done when heparin contamination during specimen collection is suspected (

3、not when the patient is known to be receiving heparin therapeutically).principleprotamine is a very positively charged peptide. it binds to heparin, a very negatively charged molecule, to form a stable ionic compound which does not have anticoagulant activity. heparins affinity for protamine sulfate

4、 is greater than its affinity for antithrombin iii (at), a nonvitamin k-dependent protease anticoagulant. atiii anticoagulant affects are markedly potentiated when heparin binds to atiii. subsequent to adding protamine sulfate to plasma containing heparin, the heparin preferentially binds to the pro

5、tamine sulfate resulting in at iii inhibition.specimen - platelet-poor plasma (ppp) derived from whole blood (wb) collected in a plastic blood tube or siliconized glass containing 1 part buffered 3.2% sodium citrate for each 9 parts of wb. separate* (centrifugation at 2500 g for 10 minutes) from the

6、 blood cells and test the plasma as soon as possible after collection or the plasma should be frozen and stored at -70 c until ready for testing. *alternate times and forces may be used so long as the plasma platelet count is reference range but 100 seconds. if the pt is prolonged due to heparin con

7、tamination, then both the ptt and the tt should be greatly prolonged: ptt 200 seconds and the tt 100 seconds. cardiac bypass surgery patients are given protamine sulfate to reverse the anticoagulant effect of the heparin given to prevent clotting while the patient is on the heart-lung bypass machine

8、. the excess protamine sulfate in the patients plasma can impact the results of coagulation screen tests. a clue that this may be true is a shorter than reference range tt result. protamine sulfate is used only to suggest the presence of heparin by normalizing a prolonged tt result; it cannot be use

9、d for prolonged results for the aptt and possibly pt, even when they are possibly due to heparin. this is because simply the presence of protamine sulfate in these two tests gives prolonged results.references1. burns, cheryl. laboratory testing in coagulation (chapter 39). in: mckenzie, sb (ed.). cl

10、inical laboratory hematology (2004). upper saddle river, nj: pearson prentice hall; 2004. 789.2. cobel-geard , rj and hassouna, hi. interaction of protamine sulfate with thrombin.american journal of hematology (1983); 14:3,227 233.labm 419clinical coagulationfall 2008“”“”at the end, xiao bian gives

11、you a passage. minand once said, people who learn to learn are very happy people. in every wonderful life, learning is an eternal theme. as a professional clerical and teaching position, i understand the importance of continuous learning, life is diligent, nothing can be gained, only continuous learning can achieve better self. only by constantly learning and mastering the latest relevant knowledge, can employees from all walks of life keep up with the pace of enterprise development and