[生物医药论文精品]九节龙中新的三萜皂苷成分及其对人恶性胶质瘤的细胞毒性和诱导凋亡作用

1九节龙中新的三萜皂苷成分及其对人恶性胶质瘤的细胞毒性和诱导凋亡作用A1A0A3A4A2A5A6A7A8A9A10A11A7A12A13A14A15A16A17A18A19A20A16A21A22A23A24A25A26A27A28A29A30A31A32A33A34A35A36A37A38A39A40A36A41A42A27A43A39A44A45A46A47A48A28A49A32MTTA42A50A45A51A19A52A53A54A16A55A56A57A52A53A58A59A60A61A62A63A59HOECHST33342A64A65A66A50A59DNALADDERA59WESTERNBLOTA67A68A58A5A6A69A70A71A72A73A74A75A76A28A77A78A27A43A44A45A7910A34A22A23A24A25A80A46A47A48A55A81A826A83A84A46A47A48A55A27A85A86A87A88ARDIPUSILLOSIDESIII1,IV2A89ARDIPUSILLOSIDESAD36A904A91A92A93A94A95A96A97ARDISIACRISPINB7,ARDISIACRISPINA8,ARDIPUSILLOSIDEI9A89ARDIPUSILLOSIDEII10A90A98A997A898A100A101A102A103A104A105A106A107A108A109A1106A111A112A113A114A109A1108A115A116A99A117A118A119A112A113A120A90A98A1218A122A123A124A125A126A127A128A129A130A131A132A133A134A133A135A136A137A138A129A139A140A125A141A142A143A127A144A145A146A147A130A131A136A137A148A149A150A1511A152A153A154A127A128A129A130A131A132U251MGA136A137A155A156A139A157A158A159A160A161A162A163A164A165BADA166A167A168A169A170A171A172A173A174A175A176A177A178A164CASPASE8A179CASPASE3A166A167A168A169A170A180A172A173A174A175A176A177A181A182A183A184A1853A186A187A188A189A190A191A192A1931A194A195A196A197A198A199A200A201A202A203A204A205A206A196A207A208A190A189A209A210A211A212A186A213A214A215A216A217A208A1905A2186A186A190A191A219A220A208A1904A18630A221A222A198A203A204A223A224A225A200A186A213A214A215A216A205A226A227A228A229A230A231A232A233A234A235A232A236A237A238A239A240A241A242A2431A244A243A245A246A242A2432A244A247A248A249A250A251A230A231A232A238A252A253A254A255A17A24A165A0A1A2A3A4A5A6A7A8A9A10A11A12A13A14A15A16A18A19A20A21A5A22A23A25A169A26A27A28A29A30A31A32A33A11A34A35A7A36A37A38A39A40A41A42A43A5A44A45A46A47A48A49A50A180A51A52A53A54A55A56A57A58A59A60A145A61A62A1A2A145A33A11A34A35A7A145A8A9A10A11A145A64A63A145BADA145CASPASENEWTRITERPENOIDSAPONINSFROMARDISIAPUSILLA,THEIRCYTOTOXICITYANDABILITYTOINDUCEAPOPTOSISINHUMANGLIOBLASTOMAU251MGCELLSABSTRACTOBJECTIVETOSTUDYTHEANTIGLIOBLASTOMATRITERPENOIDSAPONINSFROMARDISIAPUSILLAMETHODSACOMBINATIONOFMULTICHROMATOGRAPHYASWELLASSPECTRALANALYSISWEREEMPLOYEDFORTHESEPARATIONANDDETERMINATIONOFTHECOMPOUNDSFROMTHEPLANTTHECYTOTOXICITIESAGAINSTHUMANGLIOBLASTOMAU251MGCELLSWEREDETERMINEDUSINGTHEMTTASSAYSTHEABILITYOFARDIPUSILLOSIDEIIITOINDUCEAPOPTOSISINU251MGCELLS,ASWELLASTHEINVOLVEMENTOFAPOPTOTICSIGNALINGPATHWAYSWEREALSOSTUDIEDRESULTSTENTRITERPENOIDSAPONINS110WEREOBTAINEDANDELUCIDATED,AMONGWHICHSIXARENEWCOMPOUNDSANDNAMEDARDIPUSILLOSIDESIII1,IV2ANDARDIPUSILLOSIDESAD36NINESAPONINSSHOWEDINVITROCYTOTOXICITYAGAINSTU251MGCELLSBOTHTHEINTRINSICPATHWAYOFAPOPTOSIS,MEDIATEDBYBADDEPHOSPHORYLATIONANDCLEAVAGE,ANDTHEEXTRINISICPATHWAYOFAPOPTOSIS,MEDIATEDBYCASPASE8ANDCASPASE3ACTIVATION,WEREINVOLVEDINARDIPUSILLOSIDEIIIINDUCEDAPOPTOSISCONCLUSIONTHESTRUCTURALFEATURESOFNEWSAPONINS16ARERELATIVERAREAMONGTRITERPENOIDGLYCOSIDESORSAPONINSFROMGENUSARDISIAOURSTUDIESFOCUSEDONCYTOTOXICSAPONINSOFAPUSILLAHAVEPROVIDEDVALUABLELEADSFORPURSUINGNEWANTIGLIOBLASTOMADRUGS,ANDESTABLISHEDAHOMEBASEFORFURTHERDEVELOPMENTOFTHETITLEPLANTWITHABUNDANTRESOURCESINSOUTHERNCHINAKEYWORDSARDISIAPUSILLATRITERPENOIDSAPONINGLIOBLASTOMACYTOTOXICITYAPOPTOSISBADBCLXL/BCL2ASSOCIATEDDEATHPROMOTERHOMOLOGCASPASE1A65A66A65A662A98A67A68A69A70A71A72A73A74A75A76A77A73A78A79A80A81A82A83A84A85A86A87A88A89A90A91A82A92A93A94A95A82A96A97A74A191A99A100A101A102A103A104A105A197A106A198A107A102A108A109A110A111A112A113A114A115A116A117A118A102A119A120A121A122A123A124A125A126A127A128A129A130U251MGA131A132A133A134A135A116A117A118ARDISIAPUSILLAADCA136A137A138A139A140A137A138A139A141A142A143A106A204A144A146A147A148A198A149A150A151A152A153A106A154A155A156A157A158A159A102A160A161A106A115A162A163A164A135A166A141A142A143A167A105A168A170A171A120A121A106A220A172A173A113A136A167A105A155A161A174A146A135A223A175A176A177A178A179A116A117A118A114A146A181A182A183A1843A185A186A133A102A170A171A120A121A106A227ARDIPUSILLOSIDESI,IIA187A188A189A190A192A193A102A120A121A106A194A195A196A185A199A130A200A201A202A203A102A123A124A205A162A135A182A148A195A206A207A208A174A146A110A111A209A210A211A146A106A198A107A212A213A214A215A102A216A128A129A130A217A127A174A146A136A218A102A106A195A206A114A102A170A171A120A121A219A221A184A222A224A146A181A135A225A226A228A178A229A1022A185A120A121ARDIPUSILLOSIDESI9A230II10A231A232A236A233A234A2356A237A238A239A240A241A242A243ARDIPUSILLOSIDESIII1A244IV2A244ARDIPUSILLOSIDESAD36A245A2462A237A247A248A249A250A251A252A253A254A255A29A0A234A239A242A243ARDISIACRISPINSB7A230A8A24A26A1A2A107A3A4A5A61HNMR,13CNMR,DEPT,1H1HCOSY,TOCSY,HSQC,HMBC,NOESY,ESIMS,MS/MS,HRESIMSA62A34A7A8A9A10A11A12A13A14A15A16A17A18A140FIG1A19MTTA37A20A21A22A23A242A90A25A276A91A28A30A54A25A278A31A32A33A35A36A38A28A30A39A50A67A408A41A42A43A44A45A46A47A48A49A51A52U251MGA53A55A56A57A56A58A53A55A59A48A60A102A44A61A63A64A46A65A66A68A109A49A51A53A55A69A70A71A72A45A42A431A69A73A74A75A76A77A78A79A80A81A82A831A112A84A85A86A87A88A89A92A93U251MGA94A95A96A97A98A220A99A100A101BADA120A85A103A94A95A104A96A97A105A106A108A110A111A100CASPASE8A113CASPASE3A120A85A103A94A95A114A96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