分析测试高毅.ppt

1、A pyrene-based highly selective turn-on fluorescent chemosensor for Iron(III) ions and its application in living cell imaging,作者：Peter Kun Chung & Shi-Rong Liu & Hsuan-Fu Wang& Shu-Pao Wu,来源：JOURNAL OF FLUORESCENCE,杂志简介：Journal of fluorescence(荧光杂志)主要发表最新的荧光方面研究成果，包括荧光研究方面的进展理论，数据分析和表征等。 影响因子：2012年度

2、（2.107） 2011年度（1.789）,Abstract：,A new pyrene-based chemosensor (1) exhibits excellent selectivity for Fe3+ ions over a wide range of tested metal ions Ag+,Ca2+,Cd2+,Co2+,Cu2+,Fe2+,Hg2+,K+,Mg2+,Mn2+,Ni2+,Pb2+, and Zn2+. The binding of Fe3+to chemosensor 1 produces an emission band at 507 nm due to th

3、e formation of a Py-Py* excimer that is induced by Fe3+-binding. In addition, fluorescence microscopy experiments show that chemosensor（1）can be used as a fluorescent probe for detecting Fe3+in living cells,实验仪器,8453 UV/Vis spectrometer(Agilent,USA) DA8.3 Fourier-Transform Infrared Spectrometer(Bome

4、m,ABB,Swiss) ZEISS Axio Scope A1 Fluorescence Microscope(ZEISS,Germany),实验部分,1、Synthesis of Chemosensor 1,2、Metal ion Binding Study by UVvis and Fluorescence Spectroscopy,Chemosensor1(2M) was added with different metal ions(20M).All spectra were measured in 1.0 mL acetonitrile-acetone solution (v/v=

5、99:1).,结果与讨论：,Fig. 1 Absorption changes to various equivalents of Fe3+ in CH3CN/acetone (v/v=99:1) solutions. The excitation wavelength was 382 nm,1、紫外可见光谱,2、荧光光谱,3、红外光谱,结论,The experiment synthesize chemosensor1 from the reaction of 1-pyrenecarbox -aldehyde hydrazone and1,4-bis(2-formylphenoxy)butane to form two imine bonds between 1,4-bis-phenoxy butane and pyrene. We observed signifi