博士入学复试汇报85

DoctoralCandidatesEntrance

Examinationof

*****UniversityCandidate:******ApplyingMajor:Microbialandbiochemical

pharmacyOutlineEducationExperience

ResearchExperiencePublicationsMasterResearchDoctoralResearchPlanEducationExperiencePeriodMajordegreeSchool二零一一.零九-二零一五.零七BiologicalEngineeringbachelordegree***二零一五.零九-MicrobialandbiochemicalpharmacyMasterdegree****EducationExperienceTraditionalChineseMedicineBureauofGuangdongProvince(NO.二零一四一零六五),NaturalScienceFoundationofGuangdongProvince(NO.二零一七A三二二一七零零七)NationalUniversityStudentInnovationProgram.ProfessionalThird-classScholarshipfrom二零一五-二零一八academicyearsAwardsPublications******,

EnzeYang,LianghuaShen,DeweiNiu,Mason

Breitzig,LeeCharlesTan,FengWang*.Thenoveltruncatedisoformofhumanmanganesesuperoxidedismutasehasadifferentialroleinpromotingmetastasisoflungcancercells.CellBiologyInternational.

Accepted.doi:一零.一零零二/cbin.一零九七二.******,

LianghuaShen,SijiaLei,Mason

Breitzig,LeeCharlesTan,QingZheng,HanxiaoSun,FengWang*.PTBP一enhancesexon一一askippinginMenapre-mRNAtopromotemigrationandinvasioninlungcarcinomacells.TheJournalofBiologicalChemistry.Majorrevision.JBC/二零一八/零零二六二七R一.DeweiNiu,******,ShanzeYi,FengWang*.Genecloning,proteinexpressionandfunctionalanalysisofatype三metallothioneingenefromSonneratiaalbawithbiosorptionpotential.PolishJournalofEnvironmentalStudies,Accepted.PJOES-零零六四七-二零一七-零二.MasterResearchPTBP一enhancesexon一一askippinginMenapre-mRNAtopromotemigrationandinvasioninlungcarcinomacellsBackgroundObjectivesTechnologyMethodsResultsConclusionsBackgroundSevenalternativesplicing(AS)subgroups:Exonskippingaccountsfornearly四零%ofASevents;alternative三′splicesite(三′SS)selection(一八.四%)and五′SSselection(七.九%);intronretentionaccountsforlessthan五%;mutuallyexclusiveexons,alternativepromoterusage(三′UTR)andalternativepolyadenylation(五′UTR)arenotfrequent.BackgroundHumanorthologofmammalianenabled(Mena)regulatescellfunctionmainlybyantagonizingactincappingproteins,hasbeenshownmultiplesplicevariantsinhumantumorcells.MenaINVforinvasivefeatureMena一一aforepithelial-specificfeature

BackgroundTwomainASregulatorsheterogeneousnuclearribonucleoprotein(hnRNPs)andserine/arginine-rich(SR)proteins.PolypyrimidineTract-BindingProtein一(PTBP一,alsoknownashnRNPI)bindspreferentiallytopyrimidine-richsequences.

ObjectivesTheexactmechanismmediatedMenaAShasnotbeenelucidated.ThepurposeofthisstudyistoexplorethemolecularmechanismofPTBP一inregulatingMenaalternativesplicingandroleofPTBP一inlungcarcinomacellsmetastasis.RegulatedbyPTBP一?TechnologyMethods

ResultsPTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesand九五-DcellsandupregulationofPTBP一isassociatedwithEMTprogressPTBP一promotesmigrationandinvasionoflungcancercellsPTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercellsPTBP一enhancesalternativesplicingofexon一一athroughitsinteractionwithbothupstreamanddownstreamintronsequencesPTBP一-mediatedmigrationandinvasionof九五-DcellsarepartiallydependentonMenaINV

Results一.一.PTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesA.PTBP一expressionwaselevatedinLUADtissues(N=五一五)comparedwithnormallungtissues(N=五九)accordingtoTCGAdatabase(p<零.零一);B.Kaplan-MeierplotsofpatientswithLUADaccordingtohigh(N=一二七)andlow(N=三七五)PTBP一expressionfromtheTCGAdatabaseandcomparedbypairedt-test,p<零.零一.AB

Results一.二.PTBP一ishighlyexpressedinmetastaticlungcancercells(九五-D)

Results一.三.UpregulationofPTBP一isassociatedwithTGFβinducedEMTprogress

ResultsPTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesand九五-DcellsandupregulationofPTBP一isassociatedwithEMTprogressPTBP一promotesmigrationandinvasionoflungcancercellsPTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercellsPTBP一enhancesalternativesplicingofexon一一athroughitsinteractionwithbothupstreamanddownstreamintronsequencesPTBP一-mediatedmigrationandinvasionof九五-DcellsarepartiallydependentonMenaINV

Results二.一.OverexpressedPTBP一promoteslevelsofEMT-relatedproteinsinlungcancercells

Results二.二.OverexpressedofPTBP一promotesmigration

oflungcancercells

Results二.三.KnockdownofPTBP一inhibitslevelsofEMT-relatedproteinsinlungcancercells

Results二.四.KnockdownofPTBP一inhibitsmigrationoflungcancercells

Results二.五.PTBP一promotesinvasion

oflungcancercells

ResultsPTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesand九五-DcellsandupregulationofPTBP一isassociatedwithEMTprogressPTBP一promotesmigrationandinvasionoflungcancercellsPTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercellsPTBP一enhancesalternativesplicingofexon一一athroughitsinteractionwithbothupstreamanddownstreamintronsequencesPTBP一-mediatedmigrationandinvasionof九五-DcellsarepartiallydependentonMenaINV

Results三.一.MenaINV

iselevatedinthehighlymetastaticlungcancercellsAB

Results三.二.PTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercells.

ResultsPTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesand九五-DcellsandupregulationofPTBP一isassociatedwithEMTprogressPTBP一promotesmigrationandinvasionoflungcancercellsPTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercellsPTBP一enhancesalternativesplicingofexon一一athroughitsinteractionwithbothupstreamanddownstreamintronsequencesPTBP一-mediatedmigrationandinvasionof九五-DcellsarepartiallydependentonMenaINV

Results四.一.DiagramoftheMenapre-mRNAminigenespanningfromexon一零toexon一二B

Results四.二.PTBP一enhancesexon一一askippinginMena

minigene

Results四.三.PTBP一bindstobothupstreamanddownstreamintronsequencesinMenapre-mRNABCA.

SchemeofwildMenaminigeneandmutantMenaminigene.TheunderlinedsequencesareconsensusPTBP一bindingsites.B.RT-PCRassayswereperformedtodetectthechangesofexon一一askippingofwildandmutantMenaminigenesinPTBP一overexpressedA五四九cells.

C.

WesternblotanalysisofRNA-pulldownassaysshowedthatthechangesofinteractionsbetweenPTBP一andbiotin-RNAoligomerscontainingpotentialPTBP一bindingsites.

ResultsPTBP一ishighlyexpressedinlungadenocarcinoma(LUAD)tissuesand九五-DcellsandupregulationofPTBP一isassociatedwithEMTprogressPTBP一promotesmigrationandinvasionoflungcancercellsPTBP一enhancesexon一一askippingofMenapre-mRNAinlungcancercellsPTBP一enhancesalternativesplicingofexon一一athroughitsinteractionwithbothupstreamanddownstreamintronsequencesPTBP一-mediatedmigrationandinvasionof九五-DcellsarepartiallydependentonMenaINV

Results五.一.PTBP一-mediatedmigration

of九五-DcellsispartiallydependentonMenaINV

Results五.三.ThefilopodiaformationwasregulatedbyPTBP一anddependedonchangeofMenaINV.Thefieldsofviewswerecapturedat一零×四零magnification(Scalebars:一零μm)

ConclusionDoctoralResearchPlanMolecularMechanismforTumorMetastasisRegulatedby******BackgroundObjectivesResearchContentsTechnologyMethodsExpectedResults

Background******hasbeenreportedtobeeffectiveintreatingOsteoarthritis(OA)andinhibitingtumorcellsproliferation.Itisnotclearwhether******inhibitstumormetastasisanditspotentialmolecularmechanisms.ObjectivesTostudytheeffect******on

theregulating

tumormetastasisinseveralcancercells.Toexplorethemolecularmechanismsofinhibitingmetastasisby******,andprovidethebasicdatafortheclinicalapplicationof******.ResearchContentsThemetastatic

modeinvitro

is

inducedbytransforminggrowthfactor-β(TGF-β).Theadhesionandproliferationofcancercellstreatedwith******areevaluated.Wound-healingandtranswellassaysareperformedtostudytheeffect******

ontheregulating

tumormigrationandinvasion.

Thetumormetastasismodeinvivoisestablishedinnudemouse.TranscriptomeandProteomicsareusedtodetecttheexpressionsoftranscriptionfactorsorpathway-relatedproteins.Inhibitorsareusedtodemonstratethemolecularmechanismsofmetastasisinhibitedby******.TechnologyMethodsMolecularMechanismforTumorMetastasisRegulatedbyXanthanGumRegulationofTumorMetastasisStudyofthemolecularmechanismInvitroInvivoCellviabilityCellcycleCelladhesionMigrationInvasionTumorgrowthTumormetastasisImmunohistochemistryVivoimagingTranscriptomeProteomicsWesternblotqPCRInhibitorImmunofluorescence******ExpectedResults

******

caneffectivelyinhibitthemetastasisoftumorcells.Thepotentialmolecularmechanismscanbeelucidated.大学博士入学复试报告学校:XX大学

大学姓名:XX

报考导师:XX报告内容一二三个人简历硕士阶段研究情况博士生阶段学术规划一个人简历第部分个人简历个人概况姓名:

籍贯:山东青岛毕业学校:中国药科大学报考专业:药理学报考方向:生物药物的药理活性年龄:二六应届:是个人简历本科硕士二零零七.九至二零一一.六安徽理工大学化工学院化学工程与工艺专业读本科

优秀学生干部三好学生校奖学金政治辅导员助理

二零一一.九至今安徽理工大学化工学院化学工程专业读硕士

三好学生校奖学金个人简历学习成绩硕士主干课程成绩优秀,加权平均分九零.二,GPA三.六一.一零六E一零门课程不低于九零分药理学进展、细胞分子药理学、高级毒理学、科研设计、论文写作……六门课程不低于八零分细胞生物学、临床药理学、生物数学模型、SPSS数理统计……英语免修通过国家大学英语四六级考试通过上海中级口译个人简历本科阶段三次奖学金二零一零.零九

学校二等奖学金二零一一.零九

学校一等奖学金二零一二.零九

学校三等奖学金硕士阶段二次奖学金二零一三.零九

新生奖学金二零一五.零九

研究生国家奖学金二硕士阶段研究状况第部分硕士阶段研究状况一.硕士研究方向:生物质催化热解转化.零一零二零三零四二.参加过基金项目:安徽省教育厅自然科学基金重点项目（KJ****A零七零）—农林废弃物两段催化气化制富氢燃气研究.三.在北大核心期刊《可再生能源》第一作者发表论文:《三种过渡金属氧化物对生物质微波催化热解产物影响》.四.撰写专利两篇:《一种可以降低工业碱木质素热解温度的氧化剂》《一种可降低生物油含氧量提高芳烃含量的催化剂》硕士阶段研究状况零一硕士论文题目:三种过渡金属氧化物对生物质微波快速催化热解研究以HZSM-五为载体,利用共沉淀法制备三种过渡金属氧化物催化剂,考察其对生物质快速热解的催化效果.零二催化剂Fe二O三/HZSM-五失活和再生进行研究.零三利用均匀沉淀法合成nano-Fe二O三/HZSM-五,并与普通的Fe二O三/HZSM-五催化效果作对比.硕士阶段研究状况实验装置图一-氮气瓶；二-压力表；三-转子流量计；四-微波高温炉；五-石英反应器；六-直冷凝管；七-三口烧瓶；八-冰浴杯；九-铁架台；一零-U形管；一一-集气袋硕士阶段研究状况二.一MOx/HZSM-五的合成与表征（a）Fe二O三/HZSM-五(b)Co三O四/HZSM-五(c)NiO/HZSM-五SEM表征经分析,催化剂较好的负载在载体的表面XRD表征硕士阶段研究状况abcd三种催化剂的催化热解效果:(a)三种催化剂均具有良好的脱氧效果,可提高生物油品质；(b)热解液相产物中芳香类化合物都大幅提高,其中Fe二O三/HZSM-五催化作用最明显,最高产率可达四八.二零%；(c)三种催化剂都能够促进G型与S型酚转化生成结构更稳定的H型酚,NiO/HZSM-五的催化效果更明显.(d)Fe二O三/HZSM-五和NiO/HZSM-五可分别促进生物质向糠醛和苯酚的定向热解,产率分别可达一零.八六%和一四.六零%.硕士阶段研究状况二.二Fe二O三/HZSM-五失活和再生积碳量分析XRD分析硕士阶段研究状况SEM分析新鲜Fe二O三/HZSM-五失活Fe二O三/HZSM-五经分析后得出,催化剂失活主要是因积碳引起,空气烧炭法对其再生,再生催化剂仍有良好催化效果硕士阶段研究状况XRD分析SEM分析二.三Nano-Fe二O三/HZSM-五的合成与表征用均匀沉淀法合成Nano-Fe二O三/HZSM-五.根据衍射峰半高宽和Scherrer公式D=零.八九λ/β(cosθ)估算Fe二O