DB34∕T 4223-2022 黄缘闭壳龟　地标

34Yellow-marginedBoxTurI本文件按照GB/T1.1—2020《标准化工作导则第1部分：标准化文件的结构和起草规则》的规定增、方国侠、沈保平、高凌、徐丽、彭步旭、鲍鸣、张四刚1黄缘闭壳龟本文件给出了黄缘闭壳龟（CuoraflavomarginataGray，1863）的名称与分类、主要形态构造特征、生长和繁殖特性、遗传学特征、检测方法GB/T18654.2养殖鱼类种质检GB/T18654.12养殖鱼类种质检验GB/T18654.15养殖鱼类种质检验第4名称与分类4.2别名4.3分类5.1.1外形体呈椭圆形，前部略窄、后部略宽。头光滑无鳞，鼓膜圆而清晰，头部背面呈橄榄色，两眼后各25.1.2.1背甲35.1.2.3g，背甲长104.6mm～121.2mm，年龄4+～6+。各注：各可量性状说明参长，胃分化明显，十二指肠上附有条形白色胰脏，无盲肠。雌雄异体，雄性睾丸1对，淡白色，卵圆形，位于肾脏前端内侧，后端为输精管，通泄殖腔，阴茎位于尾基部的泄殖孔内；雌体卵巢1对，位4于肾脏前端内侧，紧靠椎板，呈黄色片状，内有粒状卵子，产卵期具多个6.1生长不同年龄组的黄缘闭壳龟体长与体重的实测值变表2黄缘闭壳龟不同年龄组实测背甲长+注：生长方程和背甲长与体重关系式参加附录A6.2繁殖7.1.1体细胞染色体数：2n=52；染色体臂数（NF）：70；核型公式57.2分子遗传学特征黄缘闭壳龟线粒体16SrRNA基因片段的碱基序列为1596bp％，8.2性状测定8.3年龄鉴定8.4细胞遗传学检测69判定规则7Wt1=12.73e0.476（r=0.9843）·················································Lt=45.47T0.466（r=0.9944）········································Wt2=34.81T0.461（r=0.9939）·····················································(A.3)Wt2——t齢时龟背甲宽，单位为毫米（mmWt1=Lt3.018（r=0.9899）··························································(Wt1——t齢时龟体重，单位为克（gLt=0.82Wt21.191（r=0.9925）·············································Wt2——t齢时龟背甲宽，单位为毫米（mm8取黄缘闭壳龟肌肉组织，剪碎，10％蛋白酶K消化，DNA提取试剂盒B.2.1扩增引物序列为16SF:5'-CACAATAAACAAACAGATGAGGC取5μL扩增产物上样到1.0％的琼脂糖凝胶进行电泳分），利用Kimura两参数模型（Kimura2param9黄缘闭壳龟线粒体16SrRNA基因标准序列（1596b1GCAAGAACCTAGCCCAACCAAAATAAAAAACTTAACAAACAAAACTATCC51CACCAAAACCAACCAAAACATTCTCACCATCCTAGTATAGGCGATAGAAA101AGACAGTTGGAGCAATAGAGATAGTACCGCAAGGGAAAGATGAAAAACAA151TGAAATATTCACTAAGCCATAAAAAGCAAAGATTAATCCTTATACCTTTT201GCATCATGATTTAGCTAGCATATTCAAGCAAAGAGCCCTAAAGTATGAAA251CCCCGAAACTAAGTGAGCTACTTAAAGGCAGCCACACCAAGGCTAAATCC301GTCTCTGTGGCAAAAGAGTGGAAAGACCTGTAAGTAGAGGTGAAAAGCCT351AACGAACTTAGTGATAGCTGGTTGCTCAATAAAAGAATATAAGTTCAACC401TTAAACCTTCTAAAAACAACCTAAAGTATAAAAGAAAAGTTTAAGATTTA451TTCAATTGGGGTACAGCCCAATTGAAAAAGGATACAACCAATAACGGAGG501ACAAAACCCCAAAAATAACAACCGTAGGCCTTAAAGCAGCCACCAACTAA551GAAAGCGTCAAAGCCCACCCGCTATTAAATAGTAACAAAAAATTTTTTCC601CCTAATAATATTGAGCTACTCCATTTAAATAGAGGAATTAATGCTAAAAT651GAGTAACAAGAAGACAAAACTTCTCTAATACGCTAGCTTAAGTCATAACA701GATAAACTACTGACTATTAACATCCATCACTATAAAACAACAACATTTTA751AATCACCCTATAGACCTAACTGTTAACCCAACACAGGAGCGTATACAAGA801AAGATTAAAATTTGTAAAAGGAACTAGGCAAATAAACGAGCCCGACTGTT851TACCAAAAACATAGCCCCTAGCAACACAAGTATTAGGGGTAATGCCTGCC901CAGTGACACTGTTAAACGGCCGCGGTATCCTAACCGTGCAAAGGTAGCGT951AATCACTTGTCTTTTAAATAAAGACTAGAATGAATGGCCAAACGAGGTTC1001TACCTGTCTCTTACAAATAATCAGTGAAATTGATCTTCCTGTGCAAAAGC1051AGGAATAACATTATAAGACGAGAAGACCCTGTGGAACTTCAAATACAAAT1101CAACTATTATCAACACCCACCTACGGGTCCACATCAAACTAGCATCTGAC1151TTATATTTTCGGTTGGGGCGACCTCGGAGTAAAATAAAACCTCCGAAAAA1201GAATTTCCTTTTAAACCTAGACCTACAGCCCACAGTGCTTCCAGCAAAAC1251GATCCAATATACTTGATCAACGAACCAAGCTACCCCAGGGATAACAGCGC1301AATCCCATCCTAGAGTTCCTATCGACGATGGAGTTTACGACCTCGATGTT1351GGATCAGGACATCCTGATGGTGCAACTGCTATCAAGGGTTCGTTTGTTCA1401ACGATTAACAGTCCTACGTGATCTGAGTTCAGACCGGAGCAATCCAGGTC1451