《细胞工程学-Cytotechnology》课程教学大纲

SyllabusofBiotechnologyatHaideCollege

Cytotechnology

Description

Cytotechnologyisakindofbiotechnologyatcellularlevel.Itcanbedividedintotwopartsofplantandanimal.Forplantcytotechnology,thefollowingcontentswillbeintroducedincluding:thehistoryofplantcell,tissueandorganculture,thetermofcelltotipotency,laboratoryfacilitiesoftenused,aseptictechniques,mediacompositionandpreparation,initiationandmaintenanceofcallus,inductionoforganogenesisandsomaticembryogenesis,suspensioncultureofdispersedplantcells,cultureofisolatedroots,antherandpollen,andprotoplasts,micropropagationbybudproliferation,protoplastfusionandsomatichybridization,cryopreservationofgermplasm,theprincipleandprotocolsofoftenusedgenetransfermethods,achievementintransgenicplantsandthebiologicalsafety,etc.

Foranimalcytotechnology,thefollowingcontentswillbeintroducedincluding:thehistoryofanimalcellculture,biologicalfeaturesofculturedcellsincludingmorphology,attachmentandgrowthcurve,equipmentsusedinanimalcellculture,componentsofanimalcellmedium,preparationandsterilizationofmedium,serum-freemedium,primarycellcultureandsubculture,cellline,cellcloningandselection,transformationofculturedcells,characterizationofcelllines,contaminationofculturedcells,cryopreservationofculturedcells,cytotoxicity,embryonicstemcells,nuclearreprogrammingandinducedpluripotentstemcells,organculture,histotypiccultureandorganotypicculture,animalcellfusionandhybridization,technicalprinciplesandprotocolsofanimalcelltransfection,transgenicanimals,genomeeditinglikeTALEN,CRISP/Cas9andNgAgo-gDNA,andproductionofclonedanimals,etc.

Pre-requisites

CellBiology

IntendedLearningOutcomes

Afteryoufinishthiscourse,youwillunderstandtheprinciplesandprotocolsofaseriesofadvancedtechniquesoncytotechnology.Andalsoyourskillsonreading,understanding,writingandspeakingacademicEnglishwillbesignificantlyimproved.

LearningSchedule

•Chapter1IntroductionforPlantCell,TissueandOrganculture

•Chapter2LaboratoryFacilities

•Chapter3AsepticTechniques

•Chapter4MediaCompositionandPreparation

•Chapter5InitiationandMaintenanceofCallus

•Chapter6Organogenesis

•Chapter7CellSuspensions

•Chapter8SomaticEmbryogenesis

•Chapter9CultureofIsolatedRoots

•Chapter10MicropropagationbyBudProliferation

•Chapter11AntherandPollenCultures

•Chapter12IsolationandCultureofProtoplasts

•Chapter13ProtoplastFusionandSomaticHybridization

•Chapter14CryopreservationofGermplasm

•Chapter15ProductionofSecondaryMetabolites

•Chapter16TransgenicPlants

•Chapter17IntroductiontoAnimalCellCulture

•Chapter18BiologyofCulturedCells

•Chapter19EquipmentsforAnimalCellCulture

•Chapter20MediaforAnimalCells

•Chapter21PreparationandSterilizationofMedia

•Chapter22Serum-FreeMedia

•Chapter23PrimaryCellCulture

•Chapter24CellLines

•Chapter25CellcloningandSelection

•Chapter26TransformationofCulturedCells

Chapter27CharacterizationofCellLines

Chapter28ContaminationofCulturedCells

Chapter29CryopreservationofCulturedCells

Chapter30Cytotoxicity

Chapter31EmbryonicStemCells

Chapter32NuclearReprogrammingandInducedPluripote