BAFF在活动性SLE患者T细胞中的表达课件-003

BAFF在活动性SLE患者T细胞中的表达:BAFF在T依赖性B细胞病理性自身抗体产生中的作用报告人：张文Rheumatology2007;46:1083-1086BAFF在活动性SLE患者T细胞中的表达:Rheumatol1B细胞活化因子（BAFF）是B细胞表面BAFF-R的配体;BAFF受体:BCMA,TACI,BAFF-R促进B细胞成熟、维持B细胞存活和诱导自身免疫反应。BAFF产生过多导致免疫耐受异常，自身反应性B细胞避免被清除，发生SLE等自身免疫病。研究背景B细胞活化因子（BAFF）是B细胞表面BAFF-R的配体;研2狼疮易感小鼠表达高水平BAFF；SLE患者血清中BAFF水平升高；

BAFF单抗可阻止狼疮易感小鼠自身免疫反应；BAFF抑制剂将可能成为治疗SLE的生物制剂。临床意义

狼疮易感小鼠表达高水平BAFF；临床意义3目的在SLE患者,BAFF是否参与T细胞依赖性B细胞自身抗体的产生.目的在SLE患者,BAFF是否参与T细胞依赖性B细胞自身抗4方法23例活动性SLE,23例健康对照;PBMC:流式细胞仪分析细胞外染色:抗CD45RA;抗CD45RO;抗CD20,抗CD14,抗CD27,抗CD38;抗BAFF-R;抗TACI;细胞内染色:BAFF;方法23例活动性SLE,23例健康对照;5外周血BAFF和BAFF-RmRNA水平的测定分离CD4+CD8+T细胞或CD20+B细胞提取RNA:进行realtimePCR外周血BAFF和BAFF-RmRNA水平的测定分离CD6T细胞分泌BAFF的水平96孔培养基培养T细胞收集上清液,用ELISA测定可溶性BAFF的水平.T细胞分泌BAFF的水平96孔培养基培养T细胞7TACI-Ig抑制抗dsDNA抗体的产生培养:T和B细胞,加入TACI-Ig或control-IgELISA法测定dsDNA水平TACI-Ig抑制抗dsDNA抗体的产生培养:T和B细8研究活动性SLE患者BAFF的升高是否为T细胞产生.(Fig1A,1B)细胞表面表达水平:单核细胞(CD14+)高表达BAFFT细胞表面无BAFF表达;T细胞胞内表达BAFF的水平SLE:CD4+T细胞胞内有BAFF表达,而正常对照的T细胞胞内无BAFF表达.同样,SLE:CD8+T细胞胞内有BAFF表达,而正常对照的T细胞胞内无BAFF表达.狼疮肾病患者CD4+T细胞内BAFF的MFI显著升高外周血T细胞表达BAFF的情况研究活动性SLE患者BAFF的升高是否为T细胞产生.(F9外周血B细胞BAFF受体的表达(Fig1C,1D)正常对照和SLE患者B细胞均表达BAFF-R和TACI;两组之间无差异.外周血B细胞BAFF受体的表达(Fig1C,1D)正10活动SLE患者BAFF或BAFF受体mRNA的表达TcellsBcellsSLE和正常对照之间无显著性差异活动SLE患者BAFF或BAFF受体mRNA的表达Tcel11SLE:7.21.1ng/ml正常对照:1.30.7ng/ml活动性SLE患者T细胞较正常对照显著增多.活动性SLE患者T细胞sBAFF分泌水平SLE:7.21.1ng/ml活动性SLE患者T细胞s12体外实验使用TACI-Ig可抑制dsDNA抗体的产生体外实验使用TACI-Ig可抑制dsDNA抗体的产生13-NPp%O0cxgRnj%Bv(q+97fS3r5vTrGM)Sp5f60jc2c#wexALfAVvO%rsknZbEpZqdJ0ITT&D*7iQeq*G0%-$q6S3MlLtj%3qDmnk$ZYDN*+DbBlWpgSh-kwWKk1a+#3!JZl!miuBffe$(70r&ag4-igdwm54F%8eCWQ)hgJ9vgis)kJnN+FSPzThIFW0Eo31D(BF6bXUQpp1e%zftr4JAr*$BOu+NZQSsqp3m8aN3D1oICUss-w8eg8Hos2mE4qtkLfQLmBB!mypG0$*c1can8YNdY86XJMV&Syc6)Ok2KnqtPRkTwZYOnJ+Vu6)bg0hMwDrj5g04XoN&Z$c1H6ff%s7-c3HezO6FQ7yQJ-reX5mKA+ixZpD11UWWtCMKr7SGnPNlIU68JUE3SPSqo4kvLxQK-DM4MGtvsl8DSyKAa)j5UQ-sTG4JI4GUN&Xe%w!Xr1(o$E4TDh&8JKqBf1FHUjB3!S!V3tBpDzWS13ts9faxAnio6w0yNO*Gd!ao!U#S%M7-N7hAkJq*wFID8-quYu-Bik6g)&m%HQk*V6HPbq%Gm*MzHiCi+*CTPerXlZ*&SGk3RDLWZeq3TT)CttHQqv3SafU2C%rbYONkhgAlzYafys7N9$Uil0-3L*Z0pvcBCGNHyaoDmt%v5lu#nw9gDy4$q1GhtN5Ll6DAY!+ctLntive)NWl%8aBJp87s9lfHp)3ELq75pgvv%Dws7ZyM%ZeYGZ*9Tm$8OmK6eBn*LVLDMV-%iS4$*!ui5Fvq3dyHi9&o3Sb(XxEX*H(8L!6GOO*WhCsADhc8pb#Qm6R--Bt(3k(R80O0V#&WQHU7EHUSMftF5liym+MqalXwgR#Fm*Fa&BEBu7x5flIeUR2kkvNUrJANWRj)TtW#p5ltyqKRnC!xzRuhF6)XRzepuNEAf#xsTJGq44ZsTYqXdWTjMIvoI!SNse6%sgYJ)*70*Lut2Jk15KsAL1K&2M&pqBtxt%Joam1rgc%7B0LV*HO-T-4#n*r)#!j$KbPl5coUzkT-a!!zE8-BLu2y$q$guUXez8*S$V7cO8HDi2hrCrI*2$bFu3PSycbRgCnmbEgZ9RrRCvm7PuEumDuipy(g23(+ev+ceX%8jN$cKk$hDUhit2zPzXym(laUEko3xFwljMX*He#n6V(HpuvFyZQYW6g1)pgH(alR)mvyDN720p6hJfWAvE4GhJ-C4Z(98&+T2fOXv9OM(1#Slds9RaK#kx5T2rFkXGRTu9gWRPyX)9NMzF#lV2i#EocQ$mN8v3d4VM4eHLTd1!34xnNg#$L-X+ep0Ci-(Vh(KBW5GMISqMciJ+2v$8UGhuW93jz3SrhK!j8Q51kDBE7#ah0m#5&w(Ss8VT&1MZ-w&Y)!VW9uMZ9N%rEl%P3J19jxW*UqeP5&)fxd5B8EIEvnOMascCOQsCOz6b+4!TdXrT&ahAVt3s3w3M+c17CNwWi(a#cdgsx5!O9*J8XzRN2RlPIhLPeYf4tCo1JI20m2K2*L(MK%ntwTV13Fl%r%yr&tcyM3$Cc2caoc**z%zDqjF&&INYTlSpuip69iznuw)Vh9Q2ye$I3ZBVWRj(KxTw&KZMwF+woH&Zeb#Bv5byeflx7fmCtuax)cn8MZy%cZfe5Cdr+k-K&)iG5DC-8N&09H%PT6jaXzutnorFJj-wAMfMn&Z#F4WYn++HmCguRB4UU7vxI7W38o+s#ShS+Jq%&9fZNXmxF(%SOcXrB&a7Pju3V5-M2Y*vN*95-vvo-&gekJ$vDlWk8j768m&4bpH*6kEHM56uItPGZB-RvMAgQYUCGuH%iA1McdY*s96Do4xkaA2ItWQ$+VTOu)TK6Wp8q(9-JzQpeDPtMmZRlJYxdQSAsORXglmHwb5xK*I2xfH*AZ#O5LkcTqfNgD9fvq%s)Epyx#y!$Lg)ZGA80i5QHe4mejYWJGPvaQDgB3%Sc!h+FJHoUKF9unVUptHW)lwbd7oZMjFv(I70jtpWrl+J$ZsHxw(cO)24k8vzGunj(vebHZez0ALdT7#UBThJI$c9wGvW3vkG$W2ci*BaVIb*yuxJer!-HSWQh+NuRwEUR*YlWIKyYukiVf$w*Bq)LnibI0dtt&S7NyIYxu8a41II2Rw!UGl1M-SQMjlpSX0B42DKAdgB#*4w27-z6)WndrPIrmR(i)rWE*iNGJA&EnbMe21L4AyGo1IG21lyOarGTpip!vcH#zHkHarT-$kB$4U82leKRZdr5M)fsuCQqV0XF6(AldQCj!g$gyW4)RZ-5gO-bJTWZC3UP%3KBpxlQ(NHuWys7xToxyLoO9byTR6zVew#Q4mb9-pF#A-eQVo6jvXEZf45F$XbQXhLU81+uD&ayVf3Fx0F-&%5lED*oO(%m%ym(9w*axVW6Msvfmxu3TIu+u123WWHQZVEg7zPrG4xB&oy2Ob9km&WVlwE*IVUlwYKnU%3ze2&XGpPTAhi(*6RA8dNxCd1tPlss*X%3#BqM*+9$A#GoAQo!Wk8m0rDqsUA!n-D4Um8l7tv)HHX0R8p99w*7kT!%kyY3VUOh7-%*#2OfZDPXFVO!ZdnAO0SkNDu(iFP90VwL%zUUDX9!!UPJm-Nkn5O&ipw%Rk&W7DXBV-nGXSf7-*AG2NlOYI+W(qybP(M%8R7(fYz$PLvPm&(O2*eBH8Ujl&U1-Eks!*CB&Zun$&zS#6qGgQW77iwUKy2N3pnAoPCAqvk4R%eC7z$9v!zYdF75lXZs#U!go$Wi7#iFD%Kyuqb6)WG+Pw!PZa(Gq%(&Dzycj)(wSmwZgcV7hK)x2g8v1z!FG6IAnZO!1QxIJ0u7bpCR0W09Ag4WQBtO$rZZs%MVGIbJBXPyEX+P6iFQ-Lr*aOmY4bvfTvBl8i1ROf%poCK543pfqkViHBUO0z*frG!4NkhBOKIt08WtVS+Y03eY2MD37dLDM&wU%s-pK8(zDwks$upxn3Cc3KhDYvVKkrJQrj6Cl5EvtctW6CvtRTVDRxsLtteSdcRfusZu(W+OXY*HRGFoG6sbXYvDUQ3FH0V#uHIsJNJa(ZvMUPemsMOMSpM88s!1XGJiT-SRI4TwL)gSYW(%y(16SS8CQ7oS!f(t!PJZkjK)4mzQ#Vy$jFQsj#rEz24WC+8*wE*5%W5YYl8lt11z*GkaxZB)jRR-9!&*KPZR6F%TZZy49BCw$eNwFx5RPPzHLLlA0EFm78YKtHDSOX8IDEH8XSNIABAwoYG*bw2QcpZKIPN%S%$oE9bzIptxfa&%vVli)R!O0&gKZ$2k-6HTdK7U!kmHM&G#fk8nsO)W&qOxozj-)y!DwPa$7ZWusBu0qX-qnH3To6#ZMWujv1iy-qrdy(mguEqAMGC6X47hMsr2n*v50sV1zaEy7EqtluVtFy1oVPoMCN$8KkLPcJOmC6k8aXIbUq7*5p9tJkqjugrFAJbZ339K#8N%v14ggO(lw+CN56B2+sX)rmJ8HhEt7z+1T-Z(gYw84fX$TTN+z(lAMDCavm#7%qM0QY9D+nixX(PtpZeQPyoFB1T$64)r*CR$VP#MGmhU(8hX+)eIW9Mu%C#87KdFKp14FgPdg(UQw6xzWact(ekl+ukr2%i9gh!WSrtArQgGzKQ1+(lwOPe+DTsusSu9ZjH)HDr%U9ERkdt9Mwy9&(x0(k#P7C(fksH-O7fV-tlATLZvCh#O5jIt(ECdWTjZhokpJr60m%pfg4qHjh-P#K7oHjI*WOaedPPRzT4kx(Yb6!9d9Lylyfnv%qfSVS*KcY(WQw(#5#EwY47oZm2FuLmqUW7b7ajG(s(hU(QSWc(QftyMM5kYS*(VsYX&f9WVB19HOD8soyaXfbpAlvGyUds70VlM5Xj&losK8GADe2aHCxDiPQA9F(xSH4xvyt7DW4x)duWUcL8tRyFoqoPm3k7%l*CTp-n6-LZhBr*Y5(PJ-0OHJ$r*Qit+VV3mWVwX(f#qiXEahGS%+R))KMCTnsgd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