分子生物学试卷英文

西南大学生物技术学院《 》课程试题og卷〜 学年第学期期末考试考试时间120分钟考核方式开卷笔试学生类别本科人数98适用专业或科类生物技术年级05级题号 .二三四五六七八九十合计得分签名阅卷须知：阅卷用红色墨水笔书写，得分用阿拉伯数字写在每小题题号前，用正分表示，不得分则在题号前写0；大题得分登录在对应的分数框内；统一命题的课程应集体阅卷，流水作业；阅卷后要进行复核，发现漏评、漏记或总分统计错误应及时更正；对评定分数或统分记录进行修改时，修改人必须签名。 特别提醒：学生必须遵守课程考核纪律，违规者将受到严肃处理。IDefinethemeaningofthesefollowingterms(30points,3ptsperterm)meltingtemperature(Tm)Cvalueparadoxexonsemi-conservationreplicationcDNAlibrarygelshiftassaypromoteropenreadingframe(ORF)genefamilyexpressedsequencetag(EST)IIFillinblank(10points,1ptsperblank)Thesimplestcloningvectoratleastcontains,andorielements.2.ThediagrambelowshowstwoRNAmolecules.MoleculeIisprocessedtogivemoleculeII.Answerthefollowingquestionsaboutthesetwomolecules:Wheredoesthisconversionhappen? WhattypeofRNAmoleculeismoleculeI? WhattypeofRNAmoleculeismoleculeII? Nametheregions/structuresindicatedbythearrows:IIISelecttherightanswer(20points,2ptsperquestion;Selectthebestanswerforeachquestion.)TOC\o"1-5"\h\zThebondbetweenaphosphategroupandtheribosesugargroupsinRNAiscalledwhichofthefollowing?( )(A)Phosphodiesterbond(B)Glycosidicbond(C)Peptidebond (D)AmidebondWhichenzymedoesnotplayaroleinDNAreplication?( )(A)DNApolymerase (B)Helicase(C)Single-strandedbindingprotein(D)GuanyltransferaseSplicingistheprocessthatdoeswhichofthefollowing?( )RemoveintronsandconserveexonsRemoveexonsandconserveintronsRemovemutatedregionsofprimarytranscriptRNAAddmultipleadenosinebasestotheendofaprimaryRNAtranscriptTheidealspacingbetweenthe-10and-35regionsofaprokaryoticpromoteris:( )bp.(A)14 (B)15(C)16 (D)17TOC\o"1-5"\h\zThe5'cappingprocesscreateswhattypeoflinkage?( )(A)5'-3' (B)3'-5'(C)5'-5' (D)3'-3'Codonsthatcodeforthesameaminoacidarecalled:( )(A)Synonyms (B)Similar(C)Degenerates (D)ComplementsWhichofthefollowingisnotacharacteristicofatRNAmolecule?( )(A)Darm (B)Betaarm(C)Tarm (D)Anti-codonarmWhatsignalstheendoftranscription?( )(A)Stopcodon (B)Terminator(C)TheendoftheDNAchain(D)RNApolymeraserunsoutThestartcodonis:( )(A)AUU (B)UAA(C)AUG (D)UAGWhichofthefollowingisthesequenceofthe-10regionofprokaryoticpromoters?( )(A)TATAAT (B)TTGACA(C)TATA (D)CAAIVAnalyticalquestions(30points,6ptsperquestion)Describebrieflytheattenuationofthetrpoperon.DescribetheprincipleofSanger’senzymicmethodforDNAsequencing.WhatarethebasicfeaturesofDNAdoublehelixdeducedbyWatsonandCrick?PleasedrawaschematicstructureofTTPinthefollowingspace.Youmaywritejusttheletter"T"forthebase.OnthefollowingagarosegelwithmarkerDNAasindicated:Placeaplus(+)signonthepositivepoleofthegelandaminus(-)signonthenegativepole.Drawthebandorbandsthatyouwouldseeifyoudigesteda3kbplasmidwithEcoRIandranittotheleftofthemarker.AssumetheplasmidhasaMCSwiththefollowingrestrictionsites: