分子生物学Molecular Biology知到章节答案智慧树2023年山东第一医科大学_第1页
分子生物学Molecular Biology知到章节答案智慧树2023年山东第一医科大学_第2页
分子生物学Molecular Biology知到章节答案智慧树2023年山东第一医科大学_第3页
分子生物学Molecular Biology知到章节答案智慧树2023年山东第一医科大学_第4页
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分子生物学(MolecularBiology)知到章节测试答案智慧树2023年最新山东第一医科大学第一章测试

TheNucleicacidwillgetmaximumUVabsorbanceatwavelength_____()

参考答案:

260nm

ThecommoncomponentcomposedofDNAandRNAis_____()

参考答案:

Guanine

ThesecondarystructureofDNAis_____()

参考答案:

doublehelix

ThecorrespondinganticodontocodonACGis___()

参考答案:

CGU

SinglestrandDNA5’-CGGTA-3’canannealwiththefollowRNA_____()

参考答案:

5’-UACCG-3’

mRNAisusedas_____intranslation.()

参考答案:

template

AmountofTinRNAis0.25mol,soamountofAis_____.()

参考答案:

0.25mol

tRNAineukaryoticcellcontains_____()

参考答案:

5.8sRNA;5sRNA;18sRNA;28sRNA

ThebaseinDNA_____()

参考答案:

C;T;A;G

Stem-loopisatypeofsecondarystructurenotusuallyfoundintRNA.()

参考答案:

AllgenomesarecomposedofRNA.()

参考答案:

第二章测试

SequencesthatareremovedfromtheprimaryRNAtranscriptineukaryotesarecalled________.()

参考答案:

introns

Whichcharacterisnottrueforeukaryoticgenes?()

参考答案:

Alltheprotein-codinggenesinhighereukaryoteshaveintrons.

Whichisnotafeatureofenhancer?()

参考答案:

Ageneoftenhasonlyoneenhancer.

Theregulatorsofeukaryoticgeneexpressionconsistof________.()

参考答案:

enhancer;insulator;silencer

Allhousekeepinggeneshaveweakpromotorsandareregulatedtomaintaintheirgene

productsatconstantlowlevelconcentrations.()

参考答案:

第三章测试

BasedonMeselson-Stahlexperiment,eachdaughterDNAmoleculeiscomposedof()

参考答案:

oneoldandonenewstrand

Whichmechanismcanexplaintwostrandsaresynthesizedintheoppositedirection,inthereplicationfork?()

参考答案:

semidiscontinuousreplication

Althoughmammaliangenomeishuge,cellscanreplicatethegenomeinjustafewminutes.Whichisthemostpossiblereason?()

参考答案:

Hundredsofreplicationforksworksimultaneously

WhichisTUREabouttheinitiationofprokaryoticDNAreplication?()

参考答案:

DNAhelicaseunwindsDNAduplexatoriC

Whichis

FALSEabouttheterminationofprokaryoticDNAreplication?()

参考答案:

Coordinatethesynthesisoflaggingandleadingstrands

WhichisFALSEaboutDNApolIIIinprokaryotes?()

参考答案:

α,δ,ξsubunitsformcoreenzyme

MitochondrialDNAreplicatesin()

参考答案:

D-loopreplication

RNAprimerinDNAreplication()

参考答案:

isrequiredbyallOkazakifragments;provides3’-OHtosynthesizeDNA

DNAreplicationneeds()

参考答案:

helicase;primase;topoisomerase;DNAligase

WhatarethedifferencesofeukaryoticDNAreplicationfromprokaryotes?()

参考答案:

thereplicationoriginofeukaryotesisshorterthanthatinprokaryotes;telomereandtelomeraseaidterminationofeukaryotes;eukaryoticDNAisreplicatedinSphaseofcellcycle;eukaryoticDNAcontainsmultipleorigins

TheOkazakifragmentofeukaryotesislongerthanthatofprokaryotes.()

参考答案:

ReversetranscriptaseexertsthefunctionsofRNAdependentDNApolymeraseandDNAdependentDNApolymerase.()

参考答案:

第四章测试

WhichofthefollowingDNAdamagesbelongstoexogenousDNAdamage().

参考答案:

Ionizingradiation

WhichtypeofDNApointmutationswillnotleadtothechangeinaminoacid().

参考答案:

Silentmutation

whichbasesubstitutionbelowbelongsto“Transition”pointmutation.()

参考答案:

C→T

WhichiswrongbelowasdescribedfordirectlyundoingDNAdamagesrepair.()

参考答案:

null

InwhichprocessGG-NERandTC-NERshowdifferent()

参考答案:

DNAlesionrecognition

ThedeficiencyofNERrepairproteinsissusceptibleto_disease.()

参考答案:

Xerodermapigmentosum

Duringmismatchrepairinprokaryotes,thenewlysynthesizedDNAisrecognizedbythelackof()modification()

参考答案:

Methylation

DuringdoublestrandedDNAbreakrepair,NHEJshowsmoreprecisecomparedtoHR.()

参考答案:

DNAdamageisthemaincausefortheincreasedfrequencyofgenemutation.()

参考答案:

BaseexcisionrepairisoneofDNArepairpathwaystocorrectslargebaselesions.()

参考答案:

第五章测试

WhichcomponentoftheprokaryoteRNApolymeraseholoenzymeisinvolvedinrecognizingpromotersspecifictocertaintypesofgenes?()

参考答案:

σ

WhichRNApolymerasetranscribesthegeneofinsulin?()

参考答案:

RNApolymeraseII

AlltheRNAsbelowaretranscribedbyRNApolIexcept()

参考答案:

23SrRNA

Whichofthefollowingstepsoccursfirstinprokayotictranscription?()

参考答案:

formationofaclosedpromotercomplex

TheRhoproteinisinvolvedinthe_____stageoftranscription.()

参考答案:

termination

Inprokaryotes,RNApolymerasecanbindupstreamsequencesofpromotersdirectly.Eukaryotesneedsomeproteinstofacilitatethebinding.Whatarethenamesoftheseproteins?()

参考答案:

Transcriptionfactors

WhichtranscriptionfactorcanrecognizeandbindtoTATAbox?()

参考答案:

TFIID

AmutationtothissequenceineukaryoticmRNAwillaffecttheprocessbywhichthe3'-endpoly-AtailisaddedtothemRNA.()

参考答案:

AAUAAA

Thefunctionsof5’CapofeukaryoticmRNAinclude()

参考答案:

transportofthemRNAfromnucleustocytosol;;enhancementofthemRNA’stranslatability;;protectionofthemRNAfromdegradation;

Cis-actingelementsandtrans-actingfactorscontroltranscription.Thecis-actingelementsincludetheTATAbox,enhancersandotherelementsetal.()

参考答案:

RNAPolIIistheenzymesynthesizinghnRNAineukaryotes.Itisnotsusceptibletoamanitin.()

参考答案:

Eukaryotictranscriptionsisaccompaniedbytranslation.()

参考答案:

第六章测试

Weusuallycallcodonscontains5characteristics,amongwhichthedegeneracyofthegeneticcodemeans?()

参考答案:

Mostaminoacidshavemorethanonesetofcodons

AgenehasthebasesequenceACCCGTGCACGT.IftheAatthebeginningofthesequenceisdeleted,whatwillbehappen?()

参考答案:

AllofthecodonsafterApointwillbechanged

WhichbondisanaminoacidlinkedtothetRNA?()

参考答案:

ester

Theenergyrequiredforthestepofpeptidechainelongationinprokaryoticis?()

参考答案:

onlyGTP

TheShine-Dalgarnosequenceisfoundonthe________.()

参考答案:

mRNA

Amongthefollowingfactors,whichonecoulddelivertheaminoacyl-tRNAtotheAsiteofribosome?()

参考答案:

EF-Tu

ThespecificsequenceontheNterminusofsecretoryproteiniscalled()

参考答案:

signalpeptide

Whichproteinfactorhasthetranslocaseenzymeactivityineukaryotes:()

参考答案:

eEF-2proteinfactor

WhichoftheclassesofRNAmoleculesis/arepartoftheprocessof

translation?()

参考答案:

TransferRNA;MessengerRNA;RibosomalRNA

MessengerRNA(mRNA)ismadebyaprocesscalledtranslation.()

参考答案:

Polypeptidesunfoldinthecytoplasmofacelltoformafunctionalprotein.()

参考答案:

Thegeneticcodehasfourletters,A,T,G,andC.()

参考答案:

第七章测试

WhichisNOTafinalproductofgeneexpression?()

参考答案:

mRNA

Ifageneisexpressedatrelativelyconstantrateinalmostallthecells,itiscalleda(an)()

参考答案:

housekeepinggene

Themostefficientstepofgeneexpressionregulationis()

参考答案:

transcriptioninitiation

WhichoneisNOTacis-actingelement?()

参考答案:

activatorprotein

Theprokaryoticoperatorsequenceisusually().

参考答案:

locatedontheupstreamregionofpromoter

Intheregulationoflacoperon,whenthemediumcontainsbothglucoseandlactose,whichstatementistrue?()

参考答案:

bothrepressorandCAPareinactive

Inlacoperon,repressorproteincouldbindto()sequence.()

参考答案:

operator

Intheregulationoflacoperon,whenthemediumcontainsonlyglucose,whichstatementistrue?()

参考答案:

concentrationofcAMPislow,CAPisinactive

Ifageneisexpressedonlyincertainorgansortissues,thisiscalled()

参考答案:

spatialspecificityofgeneexpression

WhichisNOTthebasalcis-actingelementsineukaryotes?()

参考答案:

operator

Enhancersmustlocateontheupstreamregionofpromoter.()

参考答案:

Theactivechromatinusuallyshowslowlevelofmethylation.()

参考答案:

第八章测试

Whichstatementistrueaboutoncogenes?()

参考答案:

Theyareusuallyactivatedincancerdevelopment

Whichoneofthefollowingisoncogene?()

参考答案:

myc

Thefollowingchangespromoteoncogeneactivation,except()

参考答案:

Lossofheterozygosity

Themechanismsoftumorsuppressorgeneinactivationinclude()

参考答案:

Mutation;hypermethylation;Lossofheterozygosity

Inmostchronicmyelogenousleukemia,tumorigenesisiscausedbyoncogeneamplification()

参考答案:

第九章测试

ThebasicstepsofPCRreactionare:()

参考答案:

denaturation,annealing,extension

ThebasiccomponentsofthePCRsystemdoesnotinclude:()

参考答案:

NTP

WhichtechnologycanbeusedtoidentifyDNA?()

参考答案:

Southernblotting

Whicharerightaboutmolecularhybridization?()

参考答案:

FromtheunderstandingofDNAdenaturationandrenaturationphenomenon;Thehybridmoleculesmustbesingle

InthePCRreaction,thepurposeofannealingistomakethecombinationofprimerandtemplate.()

参考答案:

Proteinchiptechnologyhasthecharacteristicsoffastandhighthroughput,itcanbeusedtoanalyzethousandsofproteinsinthewholegenomelevelatthesametime.()

参考答案:

第十章测试

WhichofthefollowingstatementsisnottrueconcerningTypeIIrestrictionendonucleases?()

参考答案:

TheycancleavesingleDNA

Whichisnotnecessaryaspartofprokaryoticexpressionvector?()

参考答案:

PolyAsignal

Basedonthenamingrules,thefirstletterofarestrictionendonucleasemeans()

参考答案:

Bacterialgenus

Isocaudarnerpresents()

参考答案:

Variousrestrictionendonucleasesrecognizedifferentpalindromes,butproducesamestickyends

Plasmidsareusuallyintroducedintobacterialcellsby.()

参考答案:

Transformation

T4DNAligaserequires()

参考答案:

ATP

Ininsertionalinactivationscreening,thebacteriathataresensitivetotetracyclineandresistant

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