Thymidine-3-5-diphosphate-tetrasodium-Deoxythymidine-3-5-diphosphate-tetrasodium-DataSheet-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEThymidine3',5'-diphosphatetetrasodiumCat.No.:HY-115581ACASNo.:118675-87-9Synonyms:Deoxythymidine3′,5′-diphosphatetetrasodium;pdTptetrasodium分⼦式:C₁₀H₁₂N₂Na₄O₁₁P₂分⼦量:490.12作⽤靶点:Apoptosis;MicroRNA作⽤通路:Apoptosis;Epigenetics储存⽅式:4°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性数据体外实验H2O:50mg/mL(102.02mM;Needultrasonic)DMSO:3.33mg/mL(6.79mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制备储备液1mM2.0403mL10.2016mL20.4032mL5mM0.4081mL2.0403mL4.0806mL10mM0.2040mL1.0202mL2.0403mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性Thymidine3',5'-diphosphate(Deoxythymidine3′,5′-diphosphate)tetrasodium葡萄球核酸酶和含有1的都铎结构域(SND1，MicroRNA调控复合体RISC亚)与[3,5-2H2]酪氨酸核酸酶([3,5-2H2]tyrosylnuclease)的选择性抑制剂。Thymidine3',5'-diphosphatetetrasodium具有抗肿瘤活性，在⽣化反应也可⽤1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE作催化剂。IC50&TargetStaphylococcalnucleaseandtudordomaincontaining1,SND1;[3,5-(2)H(2)]Tyrosylnuclease[1][2]体外研究Thymidine3',5'-diphosphatetetrasodium(200µM;18h)significantlyreducestheexpressionlevelofp65andp65nucleartranslocationinWTandAlb/SND1(specifictransgenicmouseoverexpressingSND1)hepatocytesbyinhibitingstaphylococcalnucleaseandtudordomaincontaining1(SND1)enzymeactivity.Thymidine3',5'-diphosphatetetrasodiuminhibitsthesphericalformationofWTandAlb/SND1hepatocytes[1].体内研究Thymidine3',5'-diphosphatetetrasodium(0.8mg/kg;i.p.;twiceaweekfor4weeks)hasinsignificanteffectonserumliverenzymes(AST,ALT,AP),totalprotein(TP),albumin(Alb),andglobulin(Glo)inWTB6/CBAmice[1].Thymidine3',5'-diphosphatetetrasodium(0.8mg/kgand1.6mg/kg;i.v.;twiceaweekfor4weeks)significantlyinhibitstumorgrowthinWTB6/CBAmice[1].Thymidine3',5'-diphosphatetetrasodium(0.8,0.16and0.32mg/kg;s.c.;twiceaweekfor4weeks)inhibitstumorgrowth,inflammatoryreactionandtheexpressionoftumorinitiatingcells(TIC)markersinadultmaleNSGmice.Thymidine3',5'-diphosphatetetrasodiumup-regulatestheexpressionofPTEN,TGFBR2andCDKN1Capoptosisandselectivetumorsuppressorgenes[1].AnimalModel:2monthsoldWTB6/CBAmice[1].Dosage:0.8mg/kg.Administration:Intraperitonealinjection;twiceaweekfor4weeks.Result:Hadinsignificanteffectonserumliverenzymes,totalprotein,albuminandglobulinandshowedbiosafety.AnimalModel:NSGmice[1].Dosage:0.8,0.16or0.32mg/kg.Administration:Intravenousinjectionorsubcutaneousinjection;twiceaweekfor4weeks.Result:Inhibitedtumorgrowth,showedantitumoractivityandimmunomodulatoryeffects.REFERENCES[1].NidhiJariwala,etal.OncogenicRoleofSND1inDevelopmentandProgressionofHepatocellularCarcinoma.CancerRes.2017Jun15;77(12):3306-3316.[2].CohenJS,etal.Protonmagneticresonancestudiesofthetyrosineresiduesofstaphylococcalnucleaseusing[3,5-2H2]tyrosine[J].BiochimicaetBiophysicaActa(BBA)-ProteinStructure,1971,236(2):468-478.McePdfHeight2/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECaution:Producthasnotbee